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The map label for this gene is dmlR [H]

Identifier: 78066828

GI number: 78066828

Start: 2419157

End: 2420080

Strand: Reverse

Name: dmlR [H]

Synonym: Bcep18194_A5359

Alternate gene names: 78066828

Gene position: 2420080-2419157 (Counterclockwise)

Preceding gene: 78066831

Following gene: 78066827

Centisome position: 65.51

GC content: 69.05

Gene sequence:

>924_bases
ATGCCGCCCCTGCGAGTCATGGACCGGTTCAAGCAGATCGAAACGTTCGCCGCGGTCGCGGCGAAGGGCAGCCTGTCGGC
GGCCGCGCACGCGGAAGGCGTCGCACCGGCGATCATCGGCCGCCGCCTCGACGCACTTGAAGAGCGGCTCGGCGTCAAGC
TGCTGGTGCGCACGACGCGCAAGCTCACGCTGACGTTCGAGGGCTCGGCGTTCCTGGAGGATTGCCAGCGCGTCATCAAC
GACATGCAGAACGCGGAGGCGAGCGTGTCCGCCGGCGGCGTCAAGGCGAGCGGCCACCTGCGCATTTCCGCGCCGGCCGG
TTTTGGCCGGCGGCACGTTGCACCGCTCGTGCCCGAATTCAGCGTCGGCCATCCGGACGTGTCGGTCACGCTCGACCTGT
CCGACCGGATGGTCGACCTCGTCAACGAGGGGTTCGATTGCGCGGTGCGGCTCGGCGAGCTGCCCGACTCGTCGCTCGTG
TCGCTGAAGCTCGGCGAGAATCGCCGCGTGTGCGTCGCTTCTCCCGCGTACCTGGCACGGCGCGGCACGCCGGCCACGCT
CGCCGAGCTGGCGAGGCACAACTGCCTCGCGCTCGCGGCAAACGCGAACCAGCAACGCGGCTGGGCGTTCCAGGAGGACG
ACAAGGTCGTGTCGATCCGCGTGAACGGCACGATGGAATGCTCGGACGGCGCGGTGCTGCACGAGTGGTGCCTCGACGGC
CACGGGCTCGCATGGCGCTCGTGGTGGGAGGTCGGCGACGACATCGCGGCCGGCCGGCTCGTCAGCGTGCTCGACGCATT
CGCGGCCCCGCCGATCGGCATCCATGCGGTGTTTCCGCAACGCCGGCATCTGCCGTTGCGCGTGCGGCTGTTTCTCGATT
ACCTGAAGCACACCTACGAACAGCCCGGATATTGGGGCGAGTAA

Upstream 100 bases:

>100_bases
TATTCGTGATCGTATGTGCTAAAAAAGACGCTAAACATCACTTGATCTTTTACTTTCATATACCTAATCTTGAGCGGTTT
AAGCTATTTGAAGGGCGGCA

Downstream 100 bases:

>100_bases
GTCGTGCATGCCCGCGCGTTTCCGATATGAGGGCGAACCCGCTGCCGGCGCCGGTTGGGCGGTTTCGCGCACTACACTCG
TAGGAGGCATGTCCGACGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPPLRVMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRVIN
DMQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSVGHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLV
SLKLGENRRVCVASPAYLARRGTPATLAELARHNCLALAANANQQRGWAFQEDDKVVSIRVNGTMECSDGAVLHEWCLDG
HGLAWRSWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYEQPGYWGE

Sequences:

>Translated_307_residues
MPPLRVMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRVIN
DMQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSVGHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLV
SLKLGENRRVCVASPAYLARRGTPATLAELARHNCLALAANANQQRGWAFQEDDKVVSIRVNGTMECSDGAVLHEWCLDG
HGLAWRSWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYEQPGYWGE
>Mature_306_residues
PPLRVMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTRKLTLTFEGSAFLEDCQRVIND
MQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEFSVGHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVS
LKLGENRRVCVASPAYLARRGTPATLAELARHNCLALAANANQQRGWAFQEDDKVVSIRVNGTMECSDGAVLHEWCLDGH
GLAWRSWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYEQPGYWGE

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=34.5890410958904, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=30.7692307692308, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=32.2033898305085, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=31.0344827586207, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=30.0353356890459, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1787589, Length=287, Percent_Identity=29.9651567944251, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789639, Length=299, Percent_Identity=28.7625418060201, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=26.8965517241379, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1790011, Length=297, Percent_Identity=23.9057239057239, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788706, Length=204, Percent_Identity=26.4705882352941, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789173, Length=262, Percent_Identity=26.7175572519084, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787601, Length=146, Percent_Identity=24.6575342465753, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33462; Mature: 33330

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLRVMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTR
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCE
KLTLTFEGSAFLEDCQRVINDMQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEF
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCC
SVGHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLAR
CCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHH
RGTPATLAELARHNCLALAANANQQRGWAFQEDDKVVSIRVNGTMECSDGAVLHEWCLDG
CCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEHHHHCCC
HGLAWRSWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYE
CCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHC
QPGYWGE
CCCCCCC
>Mature Secondary Structure 
PPLRVMDRFKQIETFAAVAAKGSLSAAAHAEGVAPAIIGRRLDALEERLGVKLLVRTTR
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCE
KLTLTFEGSAFLEDCQRVINDMQNAEASVSAGGVKASGHLRISAPAGFGRRHVAPLVPEF
EEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEEEECCCCCCCCCCCCCCCCC
SVGHPDVSVTLDLSDRMVDLVNEGFDCAVRLGELPDSSLVSLKLGENRRVCVASPAYLAR
CCCCCCEEEEEECHHHHHHHHCCCCEEEEEECCCCCCCEEEEEECCCCEEEEECCHHHHH
RGTPATLAELARHNCLALAANANQQRGWAFQEDDKVVSIRVNGTMECSDGAVLHEWCLDG
CCCCHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCEEEEEECCEEECCCCCEEHHHHCCC
HGLAWRSWWEVGDDIAAGRLVSVLDAFAAPPIGIHAVFPQRRHLPLRVRLFLDYLKHTYE
CCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHC
QPGYWGE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]