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The map label for this gene is pucI [H]

Identifier: 78066749

GI number: 78066749

Start: 2334701

End: 2336185

Strand: Reverse

Name: pucI [H]

Synonym: Bcep18194_A5280

Alternate gene names: 78066749

Gene position: 2336185-2334701 (Counterclockwise)

Preceding gene: 78066750

Following gene: 78066748

Centisome position: 63.24

GC content: 64.18

Gene sequence:

>1485_bases
ATGGCTCAGTTCAGTGTGGCGCACGACAGCGCCTATCCGGCAGAAGAAGGCGGCGAGGGCGGCGACCCGGCACTGCCGAA
TGGTTATAGCGAACGTCTGTACAACGAAGATTTGGCACCCCTGAAAAATCAGACTTGGGGTTCATACAATATCTTCGCGT
TCTGGATGTCGGACGTGCACAGCGTCGGTGGCTACGTGTTCGCGGGCAGCCTGTTCGCGCTCGGGCTGACGAGCTGGCAG
GTGCTGATCGCGCTGATCGTCGGGATCGGCTTTGTGAACCTGCTGTGCAACCTGATCGCGCGGCCGAGCCAGCAGGTCGG
CGTGCCGTATCCGGTTGCGTGCCGCGCGACGTTCGGCGTGCTCGGTGCGAACGTACCGGCCGTGATCCGCGGGCTGATCG
CGATTGCGTGGTACGGCATCCAGACCTATCTCGCATCGAGCGCGCTCGTGATCGTCGTGCTGAAATTCTTCCCGCAATGG
CTGCCTTACGCGGACGTGCATCGCTACGGCTTCCTCGGGCTGTCGGCGGTCGGCTGGGCCGGCTTCATGCTGCTGTGGGT
GCTCCAGGCGCTCGTGTTCTGGAACGGGATGGAGACGATCAAGAAGTTCATCGACTTCGCGGGGCCGGCCGTCTACGTCG
TGATGTTCATCCTCGCCGGCTACATGGTGTGGCGCGCGGGCTGGCGCAACATCGGCATCAACCTCGGCGGCGTGAAATAC
CACGGCGCGGAAGTGATTCCGGTGATGATCACGGCCGTGTCGCTAGTCGTGTCGTACTTCTCCGGGCCGATGCTGAATTT
CGGCGATTTCTCGCGCTACTGCCGCTCGTTCGGCGAAGTGAAGCGCGGCAATTTCTGGGGGCTGCCGGTCAACTTCCTCG
CGTTCTCGCTCGTCACGGTGATCACGACGGCCGCGACGTTGCCGGTGTTCGGCGAACTCATCACCGATCCGGTCGAGACG
GTCGGCCGCATCGACCATCCGACCGCGGTGATCCTCGGCGCGCTGACGTTCACGATCGCGACGATCGGCATCAACATCGT
CGCGAACTTCGTGTCGCCCGCGTTCGACTTCTCGAACGTCGCACCGCGCCTGATCAGCTGGCGCGCGGGCGGGATGCTCG
CGGCCGTCGCGTCGATCTTCATCACGCCGTGGAACCTGTTCAACAATCCGGCTGTGATCCACTACACGCTCGACGTGCTC
GGCGCGTTCATCGGCCCGCTGTACGGCGTGCTGATCGCCGACTTCTACCTCGTCAAGCGCGGCCGGCTCGTGCGCGACGA
CCTGTACACGATGTCGGAAAGCGGCACGTACTGGTACACGGGCGGCGTGAACCGCCGCGCGCTTGCCGCGCTGTTGCCGG
CCGCGGTGATCGCGATCGTCTGCGTGATGATGCCGGGCTGGCAGGGCGTCGCGAACTTCTCGTGGTTCATCGGCGCGGGG
CTCGGCTTCGTCTTCTACCGGCTGCTCGTGAGCACGAAGGCCTGA

Upstream 100 bases:

>100_bases
CCGCGGCGTTTGCCGCGGGCCGCTGGTCCGACTCCGGGCGTCGATCCGGAGCGATATCGATGACGGTGGAGCGGCCGCCC
GGCCGCGTCAAGGAGAAGTC

Downstream 100 bases:

>100_bases
GGAGCGTGCGATGAAGATTCGACTGATCAATCCGAATACGACGCAGCGGATGACCGACGCGATGGGCCGCTGCGCGCGCG
AGGTCGCGGCCGCCGGCACG

Product: permease

Products: NA

Alternate protein names: Allantoin transport protein [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MAQFSVAHDSAYPAEEGGEGGDPALPNGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALIVGIGFVNLLCNLIARPSQQVGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQW
LPYADVHRYGFLGLSAVGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKY
HGAEVIPVMITAVSLVVSYFSGPMLNFGDFSRYCRSFGEVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVET
VGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVL
GAFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGTYWYTGGVNRRALAALLPAAVIAIVCVMMPGWQGVANFSWFIGAG
LGFVFYRLLVSTKA

Sequences:

>Translated_494_residues
MAQFSVAHDSAYPAEEGGEGGDPALPNGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQ
VLIALIVGIGFVNLLCNLIARPSQQVGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQW
LPYADVHRYGFLGLSAVGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKY
HGAEVIPVMITAVSLVVSYFSGPMLNFGDFSRYCRSFGEVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVET
VGRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVL
GAFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGTYWYTGGVNRRALAALLPAAVIAIVCVMMPGWQGVANFSWFIGAG
LGFVFYRLLVSTKA
>Mature_493_residues
AQFSVAHDSAYPAEEGGEGGDPALPNGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVHSVGGYVFAGSLFALGLTSWQV
LIALIVGIGFVNLLCNLIARPSQQVGVPYPVACRATFGVLGANVPAVIRGLIAIAWYGIQTYLASSALVIVVLKFFPQWL
PYADVHRYGFLGLSAVGWAGFMLLWVLQALVFWNGMETIKKFIDFAGPAVYVVMFILAGYMVWRAGWRNIGINLGGVKYH
GAEVIPVMITAVSLVVSYFSGPMLNFGDFSRYCRSFGEVKRGNFWGLPVNFLAFSLVTVITTAATLPVFGELITDPVETV
GRIDHPTAVILGALTFTIATIGINIVANFVSPAFDFSNVAPRLISWRAGGMLAAVASIFITPWNLFNNPAVIHYTLDVLG
AFIGPLYGVLIADFYLVKRGRLVRDDLYTMSESGTYWYTGGVNRRALAALLPAAVIAIVCVMMPGWQGVANFSWFIGAGL
GFVFYRLLVSTKA

Specific function: Transport of allantoin [H]

COG id: COG1953

COG function: function code FH; Cytosine/uracil/thiamine/allantoin permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine-cytosine permease family [H]

Homologues:

Organism=Escherichia coli, GI87081749, Length=469, Percent_Identity=29.2110874200426, Blast_Score=197, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6319495, Length=482, Percent_Identity=25.7261410788382, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322219, Length=439, Percent_Identity=24.1457858769932, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319429, Length=512, Percent_Identity=23.6328125, Blast_Score=127, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6324766, Length=470, Percent_Identity=22.1276595744681, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323266, Length=470, Percent_Identity=22.7659574468085, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6324645, Length=468, Percent_Identity=21.1538461538462, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001248
- InterPro:   IPR012681 [H]

Pfam domain/function: PF02133 Transp_cyt_pur [H]

EC number: NA

Molecular weight: Translated: 53718; Mature: 53587

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCC
>Mature Secondary Structure 
AQFSVAHDSAYPAEEGGEGGDPALPNGYSERLYNEDLAPLKNQTWGSYNIFAFWMSDVH
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHH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HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 11344136 [H]