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The map label for this gene is gcvA [H]

Identifier: 78066740

GI number: 78066740

Start: 2327191

End: 2328111

Strand: Reverse

Name: gcvA [H]

Synonym: Bcep18194_A5271

Alternate gene names: 78066740

Gene position: 2328111-2327191 (Counterclockwise)

Preceding gene: 78066742

Following gene: 78066739

Centisome position: 63.02

GC content: 69.71

Gene sequence:

>921_bases
ATGGAACGCATGTGGCCCGGTACGCGCGCGCTGAGGACGTTCGATGCGGCGGCGCGGCACCTGAATTTCTCGCGGGCGGC
CGACGAGGTCGGACTGACGCCGGCGGCTGTCAGTCACCAGATCAAGGAAATCGAGGCGCGGCTCGACATGGACCTGTTCG
TCCGCACGAGCCGCAGCATCCGGCTGACGCCGGCCGGCGTCGTGCTGGCTGCTGCGGCCGCCGAGGCGCTGGCGGGGATG
CAGCGCGCGACTGCCCGGGCACGGCGCATCGCGCGGGAACAGACGGAGCTGCGCGTGTCGGTCGGCGCGCGCTTTGCGAC
GCACTGGCTGCTGCCGCGCCTGCCGCGTTTCAGGGCCGCGCATCCGGCGTTCGAGCTGTCGTTCGATATCACCGACCGGT
TGCGCGACTTCGATGACGACGATGTCGATGTGGCGATCCGCTTCGGCACCGGGCGCTATCGCGACGTGTGCGCGCAGCAC
CTGTTCGATACGTCGGTCGTTGCCGTCTGCAGCCCGGCGCTGTTGTCGGCCGGGCCGCCGTTGCGCAAGCCGCGCGATCT
GCTGCGCTACACGCTTTGCCACGCAGACTGTGAAGTCGACGGTGTGACCTGGCCGCGCTGGTCGGCGTGGATGGCCGCTG
CCGGCGTCGACGGTTTCGACGACAGCCGGTGCGTCGCGTTTCCGGATTCGAGCCACGTCGTGCAGGCCGTGACGGACGGT
ACGGCGGTCGGCCTGGCGGAGCCGCACATGATCGCGCGCGATCTCGCGGAAGGACGCGTCGTGCCGCTGTTCGACGTGAA
CGTCGAGGTGGCACCGGGCCTTGCGTATCACGTCGTGTATCCGGCGCGCACGCAGGACGACCCGCGCATCGTCGCCTTGT
GCGACTGGCTTGCGGACGAAGTTGCGACGATGCGCGCGTAG

Upstream 100 bases:

>100_bases
CGGCTCGCGGCATCTTCAAGAAAGCCTATTGTTTGCCGATTGATGGTGTCTTGAAAAACGAGTAGTTTTGGAACGGTCAT
CAAATCCGGTTTGAAGATCG

Downstream 100 bases:

>100_bases
CGGTGCCGGTGCCGGTGGCGCGTGCTATCGTGCCCGCATCTCCACCGTACGATGCCCGGTTCCGGGTGTGTTCCTTCGAT
GAGCCAACCTGAAAACAACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MERMWPGTRALRTFDAAARHLNFSRAADEVGLTPAAVSHQIKEIEARLDMDLFVRTSRSIRLTPAGVVLAAAAAEALAGM
QRATARARRIAREQTELRVSVGARFATHWLLPRLPRFRAAHPAFELSFDITDRLRDFDDDDVDVAIRFGTGRYRDVCAQH
LFDTSVVAVCSPALLSAGPPLRKPRDLLRYTLCHADCEVDGVTWPRWSAWMAAAGVDGFDDSRCVAFPDSSHVVQAVTDG
TAVGLAEPHMIARDLAEGRVVPLFDVNVEVAPGLAYHVVYPARTQDDPRIVALCDWLADEVATMRA

Sequences:

>Translated_306_residues
MERMWPGTRALRTFDAAARHLNFSRAADEVGLTPAAVSHQIKEIEARLDMDLFVRTSRSIRLTPAGVVLAAAAAEALAGM
QRATARARRIAREQTELRVSVGARFATHWLLPRLPRFRAAHPAFELSFDITDRLRDFDDDDVDVAIRFGTGRYRDVCAQH
LFDTSVVAVCSPALLSAGPPLRKPRDLLRYTLCHADCEVDGVTWPRWSAWMAAAGVDGFDDSRCVAFPDSSHVVQAVTDG
TAVGLAEPHMIARDLAEGRVVPLFDVNVEVAPGLAYHVVYPARTQDDPRIVALCDWLADEVATMRA
>Mature_306_residues
MERMWPGTRALRTFDAAARHLNFSRAADEVGLTPAAVSHQIKEIEARLDMDLFVRTSRSIRLTPAGVVLAAAAAEALAGM
QRATARARRIAREQTELRVSVGARFATHWLLPRLPRFRAAHPAFELSFDITDRLRDFDDDDVDVAIRFGTGRYRDVCAQH
LFDTSVVAVCSPALLSAGPPLRKPRDLLRYTLCHADCEVDGVTWPRWSAWMAAAGVDGFDDSRCVAFPDSSHVVQAVTDG
TAVGLAEPHMIARDLAEGRVVPLFDVNVEVAPGLAYHVVYPARTQDDPRIVALCDWLADEVATMRA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=302, Percent_Identity=35.4304635761589, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=29.7945205479452, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=27, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=28.0405405405405, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786401, Length=311, Percent_Identity=26.0450160771704, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=25.6756756756757, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789440, Length=201, Percent_Identity=26.3681592039801, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=148, Percent_Identity=31.7567567567568, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081978, Length=174, Percent_Identity=29.8850574712644, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790262, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1787589, Length=275, Percent_Identity=24, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=165, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367136, Length=131, Percent_Identity=32.824427480916, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33713; Mature: 33713

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERMWPGTRALRTFDAAARHLNFSRAADEVGLTPAAVSHQIKEIEARLDMDLFVRTSRSI
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCE
RLTPAGVVLAAAAAEALAGMQRATARARRIAREQTELRVSVGARFATHWLLPRLPRFRAA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCHHCC
HPAFELSFDITDRLRDFDDDDVDVAIRFGTGRYRDVCAQHLFDTSVVAVCSPALLSAGPP
CCCEEEEECHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCC
LRKPRDLLRYTLCHADCEVDGVTWPRWSAWMAAAGVDGFDDSRCVAFPDSSHVVQAVTDG
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCC
TAVGLAEPHMIARDLAEGRVVPLFDVNVEVAPGLAYHVVYPARTQDDPRIVALCDWLADE
CEECCCCCHHHHHHHHCCCEEEEEECCEEECCCEEEEEEECCCCCCCCCEEHHHHHHHHH
VATMRA
HHHHCC
>Mature Secondary Structure
MERMWPGTRALRTFDAAARHLNFSRAADEVGLTPAAVSHQIKEIEARLDMDLFVRTSRSI
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEECCCCE
RLTPAGVVLAAAAAEALAGMQRATARARRIAREQTELRVSVGARFATHWLLPRLPRFRAA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCHHCC
HPAFELSFDITDRLRDFDDDDVDVAIRFGTGRYRDVCAQHLFDTSVVAVCSPALLSAGPP
CCCEEEEECHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHCCCCC
LRKPRDLLRYTLCHADCEVDGVTWPRWSAWMAAAGVDGFDDSRCVAFPDSSHVVQAVTDG
CCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCCEEECCCCCCEEEEECCC
TAVGLAEPHMIARDLAEGRVVPLFDVNVEVAPGLAYHVVYPARTQDDPRIVALCDWLADE
CEECCCCCHHHHHHHHCCCEEEEEECCEEECCCEEEEEEECCCCCCCCCEEHHHHHHHHH
VATMRA
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]