Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 78066731

GI number: 78066731

Start: 2319332

End: 2319763

Strand: Direct

Name: mscL

Synonym: Bcep18194_A5262

Alternate gene names: 78066731

Gene position: 2319332-2319763 (Clockwise)

Preceding gene: 78066730

Following gene: 78066732

Centisome position: 62.78

GC content: 58.56

Gene sequence:

>432_bases
ATGAGCATCATCAAGGAATTCAAGGAGTTCGCCGTCAAGGGCAACGTCATGGATCTCGCCGTCGGCGTGATCATCGGCGG
CGCGTTCTCGAAGATCGTCGACTCGGTCGTGAAAGACCTCATCATGCCGGTCATCGGCGTGCTGACCGGCGGCCTGGATT
TCTCGAACAAGTTCGTTCTGCTCGGCACCATCCCCCCGACGTTCAAGGGTAATCCCGATTCGTTCAAGGACCTGCAGGCC
GCAGGTGTCGCCGCGTTCGGTTACGGCTCGTTCATCACGGTGGCAATCAATTTCGTCATCCTCGCGTTCATCATTTTCCT
GATGGTGAAATTCATCAACAAGCTGCGCAAGCCGGAAGAAGCCGCACCGGCCGCGACGCCGGAAGACACCGTGCTGCTGC
GCGAGATCCGCGATTCGCTGAAGCAGCGCTGA

Upstream 100 bases:

>100_bases
ATCATGCGGCCATCGGCGTGCCACCGACCCTCATTCGACGCTCTGCATCCCTGCGCGTCGCGGCACGCCTCCCCTCGCCC
CAACCAGGAGAAAGCCGCTC

Downstream 100 bases:

>100_bases
TCGCCAAGCGCCCCGCCAGCCAGGCCCGCCCCGTGCGGGCCTTTTTATTGCGCGCGCGATGCACCGCCGCATGCGGCCTA
TCTCACTTTTTTGTGACGGG

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 143; Mature: 142

Protein sequence:

>143_residues
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPTFKGNPDSFKDLQA
AGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDTVLLREIRDSLKQR

Sequences:

>Translated_143_residues
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPTFKGNPDSFKDLQA
AGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDTVLLREIRDSLKQR
>Mature_142_residues
SIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVLLGTIPPTFKGNPDSFKDLQAA
GVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEEAAPAATPEDTVLLREIRDSLKQR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=146, Percent_Identity=53.4246575342466, Blast_Score=121, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15465; Mature: 15333

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVL
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGTIPPTFKGNPDSFKDLQAAGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPAATPEDTVLLREIRDSLKQR
CCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIIKEFKEFAVKGNVMDLAVGVIIGGAFSKIVDSVVKDLIMPVIGVLTGGLDFSNKFVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LGTIPPTFKGNPDSFKDLQAAGVAAFGYGSFITVAINFVILAFIIFLMVKFINKLRKPEE
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AAPAATPEDTVLLREIRDSLKQR
CCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA