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The map label for this gene is ygfZ [C]

Identifier: 78066664

GI number: 78066664

Start: 2240368

End: 2241402

Strand: Direct

Name: ygfZ [C]

Synonym: Bcep18194_A5195

Alternate gene names: 78066664

Gene position: 2240368-2241402 (Clockwise)

Preceding gene: 78066654

Following gene: 78066665

Centisome position: 60.65

GC content: 70.14

Gene sequence:

>1035_bases
ATGAGCACACCGTTCGCTTCACCGGCAGTCCAGGCCGCACCCGCCTCGCTCCCCGTTTTCCCCCGCCCGTCCGCCGCCGA
TTTCGATGCGCCGGGCGCCTGCATGCCGTTGCCGCAGTTCGGCGTGATCGACGTGGCCGGCGATGATGCCGCGACGTTCC
TGCACAGCCAGCTCACCAACGACATCGAACATCTCGACGCCGCGAGTGCGCGGCTGTCCGGCTATTGCTCGCCGAAGGGG
CGCCTCCTCGGCTCGTTCCTCACGTGGCGCGCCGGCCACGGCGTGCGCCTGCTGGTGTCGAAGGACGTTCAGCCCGCCGT
GCAGAAGCGGCTGTCGATGTTCGTGCTGCGTGCCAAAGCGAAGCTGACGGATGCGAGCGACACGCTCGCGGTGGTCGGCT
TCGCGGGCGACGTGCGCGACGTGCTGTCGGGCATCTTCGATGCGCTGCCGGACGGCGTGCACGTGAAGGTCGACGGCCCC
GCCGGCACGCTGATCCGCGTGCCCGATGCAGCCGGCCGCAAGCGCTACCTGTGGATCGGCCCGCGTGCGGAAGTCGATGC
ACGCCTCGCCGCACTCGGCAGTTCGCTGCCGGTCGTGTCGCCGGCCGTCTGGGACTGGCTCGACATCCGCGCGGGCGAAC
CGCGCATCACGCAGCCTGCCGTCGAACAGTTCGTGCCGCAGATGGTCAACTTCGACGTGATCGGCGCCGTTAACTTTCGC
AAGGGGTGCTACCCGGGCCAGGAAATCGTCGCGCGCAGCCAGTACCGCGGCACGATCAAGCGCCGCACCGCGCTCGCGCA
CGTCGCCGGCGAGACCGATACCGTGCATGCCGGCGTCGAGCTGTTCCACAGCGACGATCCCGGCCAGCCGTGCGGGATGA
TCGTCAACGCCGCGGCCGCGCCCGCGGGCGGTGTCGACGCGCTCGTCGAGATCAAGCTCGCCGCGCTCGACAACGGCACG
GTGCACCTCGGTTCGGCCGACGGCCCGGTGCTCGCGTTCGATACGCTGCCGTACGCCTGGCCGACGGAAGCGTGA

Upstream 100 bases:

>100_bases
GCGCATTTCTTCAGTAGGGACATGGAAACGTGACTCAGGTAAGACCCATATAATACTTGCTCGCCTCCGTCAAAGTCAGG
GTTGACTGTTCCCTCATTCC

Downstream 100 bases:

>100_bases
TGCACTGACAACCCGTTCGCGCGCCGGCTCGCGAGTCGCAGCCGGCGCCCGATGTTTCCTGCGAGAGATTCGCCCGATGT
GTCTGATTGCCTTTGACTGG

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSTPFASPAVQAAPASLPVFPRPSAADFDAPGACMPLPQFGVIDVAGDDAATFLHSQLTNDIEHLDAASARLSGYCSPKG
RLLGSFLTWRAGHGVRLLVSKDVQPAVQKRLSMFVLRAKAKLTDASDTLAVVGFAGDVRDVLSGIFDALPDGVHVKVDGP
AGTLIRVPDAAGRKRYLWIGPRAEVDARLAALGSSLPVVSPAVWDWLDIRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
KGCYPGQEIVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAAPAGGVDALVEIKLAALDNGT
VHLGSADGPVLAFDTLPYAWPTEA

Sequences:

>Translated_344_residues
MSTPFASPAVQAAPASLPVFPRPSAADFDAPGACMPLPQFGVIDVAGDDAATFLHSQLTNDIEHLDAASARLSGYCSPKG
RLLGSFLTWRAGHGVRLLVSKDVQPAVQKRLSMFVLRAKAKLTDASDTLAVVGFAGDVRDVLSGIFDALPDGVHVKVDGP
AGTLIRVPDAAGRKRYLWIGPRAEVDARLAALGSSLPVVSPAVWDWLDIRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
KGCYPGQEIVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAAPAGGVDALVEIKLAALDNGT
VHLGSADGPVLAFDTLPYAWPTEA
>Mature_343_residues
STPFASPAVQAAPASLPVFPRPSAADFDAPGACMPLPQFGVIDVAGDDAATFLHSQLTNDIEHLDAASARLSGYCSPKGR
LLGSFLTWRAGHGVRLLVSKDVQPAVQKRLSMFVLRAKAKLTDASDTLAVVGFAGDVRDVLSGIFDALPDGVHVKVDGPA
GTLIRVPDAAGRKRYLWIGPRAEVDARLAALGSSLPVVSPAVWDWLDIRAGEPRITQPAVEQFVPQMVNFDVIGAVNFRK
GCYPGQEIVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAAPAGGVDALVEIKLAALDNGTV
HLGSADGPVLAFDTLPYAWPTEA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=255, Percent_Identity=28.6274509803922, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 36169; Mature: 36037

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPFASPAVQAAPASLPVFPRPSAADFDAPGACMPLPQFGVIDVAGDDAATFLHSQLTN
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
DIEHLDAASARLSGYCSPKGRLLGSFLTWRAGHGVRLLVSKDVQPAVQKRLSMFVLRAKA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
KLTDASDTLAVVGFAGDVRDVLSGIFDALPDGVHVKVDGPAGTLIRVPDAAGRKRYLWIG
HCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCEEEEEEC
PRAEVDARLAALGSSLPVVSPAVWDWLDIRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
CCCCHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHCEEEEEEECCC
KGCYPGQEIVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCCCCCCCEEECCCC
PAGGVDALVEIKLAALDNGTVHLGSADGPVLAFDTLPYAWPTEA
CCCCCCEEEEEEEEEECCCEEEECCCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure 
STPFASPAVQAAPASLPVFPRPSAADFDAPGACMPLPQFGVIDVAGDDAATFLHSQLTN
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHH
DIEHLDAASARLSGYCSPKGRLLGSFLTWRAGHGVRLLVSKDVQPAVQKRLSMFVLRAKA
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
KLTDASDTLAVVGFAGDVRDVLSGIFDALPDGVHVKVDGPAGTLIRVPDAAGRKRYLWIG
HCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCCCEEEEEEC
PRAEVDARLAALGSSLPVVSPAVWDWLDIRAGEPRITQPAVEQFVPQMVNFDVIGAVNFR
CCCCHHHHHHHHCCCCCCCCCHHHHHEECCCCCCCCCHHHHHHHHHHHHCEEEEEEECCC
KGCYPGQEIVARSQYRGTIKRRTALAHVAGETDTVHAGVELFHSDDPGQPCGMIVNAAAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECEEEEECCCCCCCCCCEEECCCC
PAGGVDALVEIKLAALDNGTVHLGSADGPVLAFDTLPYAWPTEA
CCCCCCEEEEEEEEEECCCEEEECCCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA