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The map label for this gene is rho

Identifier: 78066599

GI number: 78066599

Start: 2174154

End: 2175416

Strand: Direct

Name: rho

Synonym: Bcep18194_A5130

Alternate gene names: 78066599

Gene position: 2174154-2175416 (Clockwise)

Preceding gene: 78066598

Following gene: 78066600

Centisome position: 58.85

GC content: 60.73

Gene sequence:

>1263_bases
ATGCATTTATCCGAGCTCAAGTCTCTGCACGTCTCCGAACTGATCGAAATGGCCAATGGCCTGGAGATCGAAAACGCGAA
CCGCCTGCGCAAGCAGGAGTTGATGTTTGCCATTCTTAAAAAGCGCGCCAAGACGGGTGAGACGATCTTCGGCGACGGTA
CGCTCGAAGTGCTGCCGGACGGCTTCGGCTTCCTGCGCTCGCCGGAAATGTCGTACCTCGCGAGCACCGACGACATCTAT
ATCAGCCCGTCGCAGATCCGCCGCTTCAACCTGCACACCGGCGACACCATCGAAGGTGAAGTCCGCACGCCGAAGGACGG
CGAGCGCTACTTCGCGCTGGTGAAGGTCGACAAAGTCAACGGGCAGCCGCCCGAGGCCTCGAAACACAAGATCATGTTCG
AGAACCTCACGCCGCTGCACCCGAACAAGCCGCTGTCGCTCGAACGCGAAATGCGCGGCGAGGAAAACGTCACGGGCCGC
ATCATCGACATGATCGCGCCGATCGGCAAGGGCCAGCGCGGCCTGCTCGTCGCGTCGCCGAAGTCGGGCAAGACCGTGAT
GCTCCAGCACATCGCACACGCGATCAAGCAGAACCACCCGGACGTGATCCTGTTCGTGCTGCTGATCGACGAGCGCCCTG
AAGAAGTGACCGAAATGCAGCGCTCGGTCGCCGGCGAAGTGATCGCATCGACGTTCGACGAACCGGCCACGCGCCACGTC
CAGGTCGCCGAAATGGTGATCGAGAAGGCCAAGCGCCTCGTCGAAATGAAGCATGACGTCGTGATTCTGCTCGACTCGAT
CACACGTCTCGCACGCGCGTACAACACCGTGATCCCGGCATCGGGCAAGGTGCTGACGGGTGGTGTCGACGCAAACGCAC
TGCAGCGTCCGAAGCGCTTCTTCGGCGCGGCGCGCAACATCGAGGAAGGCGGTTCGCTGACGATCATCGGCACCGCGCTG
ATCGAAACCGGCAGCCGCATGGACGACGTGATCTACGAAGAGTTCAAGGGCACCGGCAACATGGAAGTGCACCTCGAGCG
CCGCCTCGCGGAAAAGCGCGTGTATCCGTCGATCAACCTGAACAAGTCGGGCACGCGTCGCGAAGAAATGCTGATCAAGC
CCGAGATCCTTCAGAAGATCTGGGTGCTGCGCAAGTTCATCCACGACATGGACGAAGTCGAAGCAATGGAATTCCTGCTC
GACAAGATCCGCCAGACGAAGAGCAACTCCGAGTTCTTCGACCTGATGCGCCGCGGCGGCTAA

Upstream 100 bases:

>100_bases
ATTAAAAAACGTCGACAAGACGCATTTAAGTCTCTGAGCGCTTCCGCTCGCCCTCCTCCCTTTATTTCTTTCGTCGTCTC
CTCCCTGGCGGGTTCTCCGT

Downstream 100 bases:

>100_bases
TCACCCCCGCCCCGCACCAGAAAGACGGCCGCCCGCGCATCCTGCCTGGCGGCCGTTTTTTGGTTCCAACCTAAACGTGA
ACGTACAGGTTGATCTATAA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MHLSELKSLHVSELIEMANGLEIENANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPDGFGFLRSPEMSYLASTDDIY
ISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVNGQPPEASKHKIMFENLTPLHPNKPLSLEREMRGEENVTGR
IIDMIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPATRHV
QVAEMVIEKAKRLVEMKHDVVILLDSITRLARAYNTVIPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREEMLIKPEILQKIWVLRKFIHDMDEVEAMEFLL
DKIRQTKSNSEFFDLMRRGG

Sequences:

>Translated_420_residues
MHLSELKSLHVSELIEMANGLEIENANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPDGFGFLRSPEMSYLASTDDIY
ISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVNGQPPEASKHKIMFENLTPLHPNKPLSLEREMRGEENVTGR
IIDMIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPATRHV
QVAEMVIEKAKRLVEMKHDVVILLDSITRLARAYNTVIPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREEMLIKPEILQKIWVLRKFIHDMDEVEAMEFLL
DKIRQTKSNSEFFDLMRRGG
>Mature_420_residues
MHLSELKSLHVSELIEMANGLEIENANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPDGFGFLRSPEMSYLASTDDIY
ISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVNGQPPEASKHKIMFENLTPLHPNKPLSLEREMRGEENVTGR
IIDMIAPIGKGQRGLLVASPKSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPATRHV
QVAEMVIEKAKRLVEMKHDVVILLDSITRLARAYNTVIPASGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIGTAL
IETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINLNKSGTRREEMLIKPEILQKIWVLRKFIHDMDEVEAMEFLL
DKIRQTKSNSEFFDLMRRGG

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=69.377990430622, Blast_Score=615, Evalue=1e-177,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47403; Mature: 47403

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLSELKSLHVSELIEMANGLEIENANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPD
CCHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCC
GFGFLRSPEMSYLASTDDIYISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVN
CCCCCCCCCHHHEECCCCEEECHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCC
GQPPEASKHKIMFENLTPLHPNKPLSLEREMRGEENVTGRIIDMIAPIGKGQRGLLVASP
CCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECC
KSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPATRHV
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
QVAEMVIEKAKRLVEMKHDVVILLDSITRLARAYNTVIPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLTIIGTALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINL
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCC
NKSGTRREEMLIKPEILQKIWVLRKFIHDMDEVEAMEFLLDKIRQTKSNSEFFDLMRRGG
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHLSELKSLHVSELIEMANGLEIENANRLRKQELMFAILKKRAKTGETIFGDGTLEVLPD
CCHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEECCC
GFGFLRSPEMSYLASTDDIYISPSQIRRFNLHTGDTIEGEVRTPKDGERYFALVKVDKVN
CCCCCCCCCHHHEECCCCEEECHHHHEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCC
GQPPEASKHKIMFENLTPLHPNKPLSLEREMRGEENVTGRIIDMIAPIGKGQRGLLVASP
CCCCCCCCCEEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECC
KSGKTVMLQHIAHAIKQNHPDVILFVLLIDERPEEVTEMQRSVAGEVIASTFDEPATRHV
CCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
QVAEMVIEKAKRLVEMKHDVVILLDSITRLARAYNTVIPASGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLTIIGTALIETGSRMDDVIYEEFKGTGNMEVHLERRLAEKRVYPSINL
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHCCCCCCC
NKSGTRREEMLIKPEILQKIWVLRKFIHDMDEVEAMEFLLDKIRQTKSNSEFFDLMRRGG
CCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]