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The map label for this gene is dmlR [H]

Identifier: 78066545

GI number: 78066545

Start: 2114346

End: 2115287

Strand: Direct

Name: dmlR [H]

Synonym: Bcep18194_A5076

Alternate gene names: 78066545

Gene position: 2114346-2115287 (Clockwise)

Preceding gene: 78066541

Following gene: 78066546

Centisome position: 57.24

GC content: 70.7

Gene sequence:

>942_bases
ATGGCCTCCGATCCGATTCCGGCCCCCGACAAGCCGCTCGACCTGCTCGACGTCGCGCTGTTCGTGCGCGCGGCGCTGCT
TGCGAACGTCACCGCGGCCGGGCGCGAGTTCGGCGTGTCGGCCGCGGTCGCGAGCGCACGCATCGCGCAGCTCGAGCGCC
AGCTCGGTGCGCGGCTGCTGCACCGCACCACGCGCCGCATCAGCCTGACGCAGGACGGCGAAGTCTTCATGGCGCACGCC
GATACGCTGCTGTCGGCCGCCGACGCCGCGCGCGCGTCGGTCGGCCACGGCCGCACCGAGCCGTATGGGCGGCTGAAGGT
GTCGATGTCGTCGTCGTTCGGCCGTCAGCATGTCGCGCCGGTGATTCCCGCGTTCCTGCGCCGCTATCCGTCCGTATCAC
TCGACCTGCGGCTGTCGGACGAGATCGTCGACCTCGTCGACGACGGCTACGACGTCGCGATCCGCCTCGGCGCGCTGAAG
GATTCGACGCTCGTCGCGCGCAAGCTCGCCGCAAACCGCCGCGTGCTGTGCTGCTCGCCCGCGTATCTCGCCGAACACGG
CACGCCGCGCCATCCGGCCGACCTCGCGCAGCACGAATGCGTGATCCTCGGCGACCAGCGCGACTGGTCGTTCACCACGC
CGCAAGGCCCGCTCACGGTGCGGGTCGGCGGCCGGCTCGTCGCGAGCAACGGCGAGGCGATCCGCGAAGCGCTCGTCGAC
GGCTTCGGGATCGCGATCAAGTCGACCTGGGACGTCGGCCCGCTGCTCGCGAGCGGCGCGCTCGTCACGGTGCTCGACGA
CTATCCGTTCGCGGAAGACATCGCGATCTGGGCCGTCTACCCGAGCCGCGCGCACGTTCCGCTGAAGACGCTCGCCTTCA
TCGCGTTCATGGCCGAGCATTTCGGCGATCCACCGTACTGGGATCGCGCGGACGAGCCATGA

Upstream 100 bases:

>100_bases
CTGTGGGCCGCGGCGCAGCCCCATGAACGTCATCGTAAGCAACCCGATCATTCGAGAAAACCGCTATATTTCCTGAAACA
TTTTCAGGATTTTCAGAATA

Downstream 100 bases:

>100_bases
CCGCGGCGCCGCGCGCGGTTTCGCTTCGCCCCTGACCGCCGCGTGCGGCCAACCGGCTACAATGGCGCGTTCGCCACGCA
AGGGCCCGACGCCGGACGGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MASDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEVFMAHA
DTLLSAADAARASVGHGRTEPYGRLKVSMSSSFGRQHVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALK
DSTLVARKLAANRRVLCCSPAYLAEHGTPRHPADLAQHECVILGDQRDWSFTTPQGPLTVRVGGRLVASNGEAIREALVD
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAIWAVYPSRAHVPLKTLAFIAFMAEHFGDPPYWDRADEP

Sequences:

>Translated_313_residues
MASDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEVFMAHA
DTLLSAADAARASVGHGRTEPYGRLKVSMSSSFGRQHVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALK
DSTLVARKLAANRRVLCCSPAYLAEHGTPRHPADLAQHECVILGDQRDWSFTTPQGPLTVRVGGRLVASNGEAIREALVD
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAIWAVYPSRAHVPLKTLAFIAFMAEHFGDPPYWDRADEP
>Mature_312_residues
ASDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLLHRTTRRISLTQDGEVFMAHAD
TLLSAADAARASVGHGRTEPYGRLKVSMSSSFGRQHVAPVIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALKD
STLVARKLAANRRVLCCSPAYLAEHGTPRHPADLAQHECVILGDQRDWSFTTPQGPLTVRVGGRLVASNGEAIREALVDG
FGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAIWAVYPSRAHVPLKTLAFIAFMAEHFGDPPYWDRADEP

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=38.4353741496599, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789440, Length=297, Percent_Identity=31.986531986532, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=32.0819112627986, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1789639, Length=288, Percent_Identity=29.1666666666667, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.5223367697594, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=286, Percent_Identity=30.7692307692308, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=25.177304964539, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789173, Length=276, Percent_Identity=27.536231884058, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786448, Length=280, Percent_Identity=27.1428571428571, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=192, Percent_Identity=29.6875, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=202, Percent_Identity=23.7623762376238, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33778; Mature: 33647

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHH
HRTTRRISLTQDGEVFMAHADTLLSAADAARASVGHGRTEPYGRLKVSMSSSFGRQHVAP
HHHHHEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHH
VIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALKDSTLVARKLAANRRVLCCSP
HHHHHHHHCCCCEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
AYLAEHGTPRHPADLAQHECVILGDQRDWSFTTPQGPLTVRVGGRLVASNGEAIREALVD
HHHHCCCCCCCCHHHCCCCEEEEECCCCCCEECCCCCEEEEECCEEEECCCHHHHHHHHC
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAIWAVYPSRAHVPLKTLAFIAFMAEH
CCCEEEECCCCCCHHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
FGDPPYWDRADEP
CCCCCCCCCCCCC
>Mature Secondary Structure 
ASDPIPAPDKPLDLLDVALFVRAALLANVTAAGREFGVSAAVASARIAQLERQLGARLL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHH
HRTTRRISLTQDGEVFMAHADTLLSAADAARASVGHGRTEPYGRLKVSMSSSFGRQHVAP
HHHHHEEEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHH
VIPAFLRRYPSVSLDLRLSDEIVDLVDDGYDVAIRLGALKDSTLVARKLAANRRVLCCSP
HHHHHHHHCCCCEEEEEECHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECC
AYLAEHGTPRHPADLAQHECVILGDQRDWSFTTPQGPLTVRVGGRLVASNGEAIREALVD
HHHHCCCCCCCCHHHCCCCEEEEECCCCCCEECCCCCEEEEECCEEEECCCHHHHHHHHC
GFGIAIKSTWDVGPLLASGALVTVLDDYPFAEDIAIWAVYPSRAHVPLKTLAFIAFMAEH
CCCEEEECCCCCCHHHHCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
FGDPPYWDRADEP
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]