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The map label for this gene is ywbI [H]

Identifier: 78066326

GI number: 78066326

Start: 1873716

End: 1874609

Strand: Direct

Name: ywbI [H]

Synonym: Bcep18194_A4856

Alternate gene names: 78066326

Gene position: 1873716-1874609 (Clockwise)

Preceding gene: 78066325

Following gene: 78066327

Centisome position: 50.72

GC content: 67.9

Gene sequence:

>894_bases
ATGGTGAACCCGCTTCATTTCGATCTGCAGTCGCTGCGCGTGTTCGCGCTCGTCGCCGAGCACGGCAGCCTGACGAAGGC
GGCCGAACACGGCCAGCTCACGCTGTCCGCGGTCAGCAAGCGCATCGCCGAGCTCGAAAGCGTGACGGGCAGTGCATTGT
TCGTCCGGCATGCGCGCGGTGTCGAGCTGACGCCCGCGGGCCGCGCGCTGCTCGACCATGCGGCGAAGGTGATCGAACAG
GTCAACCGGATGGCGCACGAGATGAGCGACTACGTCGCCGGTGTGCGCGGCCATATCCACGTGTGGACCAACACGTCCGC
GATCGTCCAGTTCCTGCCCGCCGATCTCGCCGCGTTCCTCACCGACAATCCGGGCATCAAGGTGAGCCTCGAGGAACGGT
TGAGCCACGAGATCGTCGACGCGCTGGCGTCCGGCAAGGCCGATCTCGGCGTGTTCGCCGACAACGTGCCGGCCCCCGGC
ATCGAACGACGGCTGTATCGGCGCGACGAACTCGTGCTGCTCGTGCCGCGCGCGCACCGGTTCGCCGCGCGCGACAGCAT
CCGCTTCGCGGATACGCTCGACGAGGACTACGTCGGGCTCAGCGACGGCAGTTCGCTGCTCGCGCGCATGACCGACGCCG
CGTTCGCGGTCGAGCGCTCGCTGAAGCTGCGGATCCAGGTATCGAATTTCGACGGCGTAAGCCGGATGATCGAGGCGGGG
CTCGGCATCGGGATCCTGCCGCGCGACGCGGTGACCGGCGAGCGCGCGGCGCGGCTCGGGGTCGTGAAGCTCGAGGATGC
GTGGGCAACCCGCACGCTGTGGGTGGGGGTGAAGGCGGGTACCGTGCTGACGACCGATATCGCGAAGCTGTTCGATTTCA
TGTCGGCGCGGTGA

Upstream 100 bases:

>100_bases
CTAGACGCGCCGTATCGCGCGGAATCGGCCGGGCGTATGATCGGCGTCAGGCCGCCGCGCTGACGGCATGCGCCGGCAAG
GTTTTCCCAGGACCCACGAC

Downstream 100 bases:

>100_bases
GGCGGGGCTTGCATTCCGTGGACGCTCGCTTATCGGCGCTTTCAAAGCGCATGGCGGCAATCGGGCACGCTGGGCAGTAG
TCGGTCGAAATGAGGCACTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARGVELTPAGRALLDHAAKVIEQ
VNRMAHEMSDYVAGVRGHIHVWTNTSAIVQFLPADLAAFLTDNPGIKVSLEERLSHEIVDALASGKADLGVFADNVPAPG
IERRLYRRDELVLLVPRAHRFAARDSIRFADTLDEDYVGLSDGSSLLARMTDAAFAVERSLKLRIQVSNFDGVSRMIEAG
LGIGILPRDAVTGERAARLGVVKLEDAWATRTLWVGVKAGTVLTTDIAKLFDFMSAR

Sequences:

>Translated_297_residues
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARGVELTPAGRALLDHAAKVIEQ
VNRMAHEMSDYVAGVRGHIHVWTNTSAIVQFLPADLAAFLTDNPGIKVSLEERLSHEIVDALASGKADLGVFADNVPAPG
IERRLYRRDELVLLVPRAHRFAARDSIRFADTLDEDYVGLSDGSSLLARMTDAAFAVERSLKLRIQVSNFDGVSRMIEAG
LGIGILPRDAVTGERAARLGVVKLEDAWATRTLWVGVKAGTVLTTDIAKLFDFMSAR
>Mature_297_residues
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARGVELTPAGRALLDHAAKVIEQ
VNRMAHEMSDYVAGVRGHIHVWTNTSAIVQFLPADLAAFLTDNPGIKVSLEERLSHEIVDALASGKADLGVFADNVPAPG
IERRLYRRDELVLLVPRAHRFAARDSIRFADTLDEDYVGLSDGSSLLARMTDAAFAVERSLKLRIQVSNFDGVSRMIEAG
LGIGILPRDAVTGERAARLGVVKLEDAWATRTLWVGVKAGTVLTTDIAKLFDFMSAR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=26.0416666666667, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790399, Length=295, Percent_Identity=26.7796610169492, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=193, Percent_Identity=28.4974093264249, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=243, Percent_Identity=27.9835390946502, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788748, Length=292, Percent_Identity=25.6849315068493, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=23.6, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=280, Percent_Identity=24.2857142857143, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI2367136, Length=241, Percent_Identity=24.896265560166, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786713, Length=137, Percent_Identity=34.3065693430657, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=143, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788481, Length=271, Percent_Identity=24.3542435424354, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082132, Length=259, Percent_Identity=26.2548262548263, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=24.0963855421687, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=253, Percent_Identity=24.5059288537549, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=25.6809338521401, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081904, Length=199, Percent_Identity=25.6281407035176, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=250, Percent_Identity=28, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788296, Length=297, Percent_Identity=23.5690235690236, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787530, Length=296, Percent_Identity=20.6081081081081, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32280; Mature: 32280

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEHHHHHHHHHHHHHCCCCEEEEEECCC
VELTPAGRALLDHAAKVIEQVNRMAHEMSDYVAGVRGHIHVWTNTSAIVQFLPADLAAFL
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
TDNPGIKVSLEERLSHEIVDALASGKADLGVFADNVPAPGIERRLYRRDELVLLVPRAHR
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHCCCCEEEEECCCHH
FAARDSIRFADTLDEDYVGLSDGSSLLARMTDAAFAVERSLKLRIQVSNFDGVSRMIEAG
HHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCC
LGIGILPRDAVTGERAARLGVVKLEDAWATRTLWVGVKAGTVLTTDIAKLFDFMSAR
CCCCCCCCCCCCCCHHHHCCEEEECCCCCEEEEEEEEECCEEHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNPLHFDLQSLRVFALVAEHGSLTKAAEHGQLTLSAVSKRIAELESVTGSALFVRHARG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEHHHHHHHHHHHHHCCCCEEEEEECCC
VELTPAGRALLDHAAKVIEQVNRMAHEMSDYVAGVRGHIHVWTNTSAIVQFLPADLAAFL
CEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHH
TDNPGIKVSLEERLSHEIVDALASGKADLGVFADNVPAPGIERRLYRRDELVLLVPRAHR
CCCCCEEEEHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHCCCCEEEEECCCHH
FAARDSIRFADTLDEDYVGLSDGSSLLARMTDAAFAVERSLKLRIQVSNFDGVSRMIEAG
HHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCC
LGIGILPRDAVTGERAARLGVVKLEDAWATRTLWVGVKAGTVLTTDIAKLFDFMSAR
CCCCCCCCCCCCCCHHHHCCEEEECCCCCEEEEEEEEECCEEHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]