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The map label for this gene is cobA [H]

Identifier: 78066272

GI number: 78066272

Start: 1815385

End: 1816134

Strand: Reverse

Name: cobA [H]

Synonym: Bcep18194_A4802

Alternate gene names: 78066272

Gene position: 1816134-1815385 (Counterclockwise)

Preceding gene: 78066273

Following gene: 78066271

Centisome position: 49.16

GC content: 72.13

Gene sequence:

>750_bases
ATGAGCCGCGCGTGGCTGATCGGCGCCGGCCCCGGTGACGCCGACCTGCTGACGCTCAAGGCCGTCCACGCGATCGGCGC
GGCGGACGTGCTGCTCGTCGACGATCTCGTGAACCCCGACGTGCTGCGCCATGCCCGCGTCGATGCGCACATCGAGCACG
TCGGCAAGCGTGGCGGACATGCATCGACACCGCAGGAGACGATCGTCGCCGCGATGCTCGTGCATCTGCGCGCGGGTCGC
AGCGTCGCGCGACTCAAGGGCGGCGATCCGTTCGTGTTCGGTCGCGGCGGCGAGGAACTGGCCGCACTCGGTGCGGCGGG
ATTCCCGGTCGAGGTCGTGAACGGCGTCACCGCGGGCATCGCCGCGCCGGCCGCGATCGGCATTCCGGTCACGCAGCGCG
GCGACGCGCAGGGCGTGATCTTCGTCACGGGCCACGGCGCGGGCGCCGACGAACCCGACTGGCGTGCGCTCGCGGCCACG
CGCATGACGATCGTCGTCTACATGGGCATCCGCAGGCTCGACGCGATTGCTGCGGCGCTGCGTGAAGGTGGTCTGCCCGG
CGATACGCCGTGCGCGGCGATCGAGAACGCGACGCGCCCCGAACAGCGGCACGTGCTCGCGACGCTCGACACGCTCGTCG
AACGCGTCGGCACGGCCGGCATCGGTTCACCGGCGATCGTCGTGATCGGCCGCGTCGCGGCGTTCGCCGACGATCCCGCG
GTACGTGCCGCACTCGGCAGCGGCGCATGA

Upstream 100 bases:

>100_bases
TCGCTCGATCCGTCATGCTTGACCTGTCTGCTGGCCGCCGCCGTGCCGCCCGCCTGCCCTGTTCCACTGTTACGACACGG
TGCGCGCCGTGCCGCGCCCG

Downstream 100 bases:

>100_bases
TGCGCGTCGCGCTCGGCGTCGGTTTCCGCGCAGGCGTGACGGCCGCGCAACTCGATGCCGCGATCCGCGCGGCGCTCGCG
CGGTACCCGGCCGCCGAACC

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSRAWLIGAGPGDADLLTLKAVHAIGAADVLLVDDLVNPDVLRHARVDAHIEHVGKRGGHASTPQETIVAAMLVHLRAGR
SVARLKGGDPFVFGRGGEELAALGAAGFPVEVVNGVTAGIAAPAAIGIPVTQRGDAQGVIFVTGHGAGADEPDWRALAAT
RMTIVVYMGIRRLDAIAAALREGGLPGDTPCAAIENATRPEQRHVLATLDTLVERVGTAGIGSPAIVVIGRVAAFADDPA
VRAALGSGA

Sequences:

>Translated_249_residues
MSRAWLIGAGPGDADLLTLKAVHAIGAADVLLVDDLVNPDVLRHARVDAHIEHVGKRGGHASTPQETIVAAMLVHLRAGR
SVARLKGGDPFVFGRGGEELAALGAAGFPVEVVNGVTAGIAAPAAIGIPVTQRGDAQGVIFVTGHGAGADEPDWRALAAT
RMTIVVYMGIRRLDAIAAALREGGLPGDTPCAAIENATRPEQRHVLATLDTLVERVGTAGIGSPAIVVIGRVAAFADDPA
VRAALGSGA
>Mature_248_residues
SRAWLIGAGPGDADLLTLKAVHAIGAADVLLVDDLVNPDVLRHARVDAHIEHVGKRGGHASTPQETIVAAMLVHLRAGRS
VARLKGGDPFVFGRGGEELAALGAAGFPVEVVNGVTAGIAAPAAIGIPVTQRGDAQGVIFVTGHGAGADEPDWRALAATR
MTIVVYMGIRRLDAIAAALREGGLPGDTPCAAIENATRPEQRHVLATLDTLVERVGTAGIGSPAIVVIGRVAAFADDPAV
RAALGSGA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=232, Percent_Identity=46.551724137931, Blast_Score=196, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6322922, Length=226, Percent_Identity=32.7433628318584, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 25269; Mature: 25138

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAWLIGAGPGDADLLTLKAVHAIGAADVLLVDDLVNPDVLRHARVDAHIEHVGKRGGH
CCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
ASTPQETIVAAMLVHLRAGRSVARLKGGDPFVFGRGGEELAALGAAGFPVEVVNGVTAGI
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCHHHHHHCCCCCCHHHHCCCHHHH
AAPAAIGIPVTQRGDAQGVIFVTGHGAGADEPDWRALAATRMTIVVYMGIRRLDAIAAAL
CCCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REGGLPGDTPCAAIENATRPEQRHVLATLDTLVERVGTAGIGSPAIVVIGRVAAFADDPA
HHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCHH
VRAALGSGA
HHHHCCCCC
>Mature Secondary Structure 
SRAWLIGAGPGDADLLTLKAVHAIGAADVLLVDDLVNPDVLRHARVDAHIEHVGKRGGH
CCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
ASTPQETIVAAMLVHLRAGRSVARLKGGDPFVFGRGGEELAALGAAGFPVEVVNGVTAGI
CCCHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCHHHHHHCCCCCCHHHHCCCHHHH
AAPAAIGIPVTQRGDAQGVIFVTGHGAGADEPDWRALAATRMTIVVYMGIRRLDAIAAAL
CCCHHCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
REGGLPGDTPCAAIENATRPEQRHVLATLDTLVERVGTAGIGSPAIVVIGRVAAFADDPA
HHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHCCCHH
VRAALGSGA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]