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The map label for this gene is kgtP [H]

Identifier: 78066224

GI number: 78066224

Start: 1749512

End: 1750816

Strand: Direct

Name: kgtP [H]

Synonym: Bcep18194_A4754

Alternate gene names: 78066224

Gene position: 1749512-1750816 (Clockwise)

Preceding gene: 78066223

Following gene: 78066226

Centisome position: 47.36

GC content: 66.13

Gene sequence:

>1305_bases
ATGGACGTCCAGACCGACCAAGCTGCGCTGTCCGCGCATGACACCCGGCGGCGCGTGTTCGCCATCGTCGGCGCCTCGTC
GGGCAACCTCGTCGAGTGGTTCGACTTCTACATCTACTCGTTCTGCGCGCTGTACTTCGCGCCGGCATTCTTCCCGAGCG
GCAACACCACGACGCAGCTGCTGAACACGGCGGGTGTGTTCGCGGCCGGCTTCCTGATGCGTCCGATCGGCGGCTGGCTG
TTCGGCCGCATCGCGGACCGCCACGGCCGCCGCAACGCGATGATGATCTCCGTGCTGATGATGTGCGGCGGCTCGCTCGT
GATCGCGGTGCTGCCGACCTATGCGCAGATCGGCGCGCTCGCACCGGCGCTGCTGCTGGTCGCGCGGCTGTTCCAGGGGC
TGTCGGTGGGCGGCGAATACGGCACGAGCGCGACCTACATGAGCGAGGTGGCGCTGAAGGGCCGCCGCGGCTTCTTCGCG
TCGTTCCAGTACGTGACGCTGATCGGCGGCCAGCTGTGCGCGCTGCTCGTGCTCGTGATCCTGCAGCAGACGCTGTCGGC
CGACGAACTGAAGGCGTGGGGCTGGCGCATTCCGTTCGTGGTCGGCGCGGCGGCCGCGCTGATCTCGCTGTACCTGCGCA
AGTCGCTCGACGAGACGTCGACGACCGCATCGCGCGACAAGAAAGACGCGGGCACGATCCGCGGCGTGTGGCAGCACAAG
GGCGCGTTCTTCACGGTGGTCGGCTTCACGGCCGGCGGCTCGCTGATCTTCTACACGTTCACGACGTACATGCAGAAGTA
CCTCGTGAACACGGCCGGCATGCATGCGAAGACGGCCAGCAACGTGATGACCGTCGCGCTGCTCGTCTACATGCTGATGC
AGCCGGTGTTCGGCGCGCTGTCCGACCGGATCGGCCGCCGCACGTCGATGATCCTGTTCGGTTCGTTCGCGGTGATCGGC
ACGGTGCCGCTGATGCATGCACTGAAGGACGTGACGAGCCCGGTGGCCGCATTCGTGCTGATTACCGTCGCGCTCGCGAT
CGTCAGCTTCTACACGTCGATCAGCGGCCTGATCAAGGCCGAGATGTTCCCGCCGGAAGTGCGCGCGATGGGCGTCGGCC
TGTCGTATGCGGTCGCGAACGCGATCTTCGGCGGTTCGGCCGAGTACGTCGCGCTGTGGTTCAAGTCGGTCGGCAGCGAG
GAAACCTTCTACTGGTACGTGACCGTGCTGTGCGCGATCTCGCTGATCGTGTCGTGGCGGATGCGCGATCCGAGCAAGGA
AGGGTATCTGCGTCACGAGCCGTGA

Upstream 100 bases:

>100_bases
AATCCCGTAGCGCCGTGCGCCGGAACCGGTGCCACGTGTGATCGTACGCGGCGGCCCCCGCCGTCTCGCCCAGAAAGCAC
TAAAAGGAACTGACCAATCC

Downstream 100 bases:

>100_bases
TCGGCTGCGCAGTGTGCATTTGAAATACGACGAACGTCCCGCCAGCCAGCGGGACGTTTTTTTTGCGGCGTGCGATTACG
CGGGTGGCGGCGGTGCGTGG

Product: citrate-proton symporter, (MFS_1)

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADRHGRRNAMMISVLMMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSADELKAWGWRIPFVVGAAAALISLYLRKSLDETSTTASRDKKDAGTIRGVWQHK
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPVFGALSDRIGRRTSMILFGSFAVIG
TVPLMHALKDVTSPVAAFVLITVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSE
ETFYWYVTVLCAISLIVSWRMRDPSKEGYLRHEP

Sequences:

>Translated_434_residues
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADRHGRRNAMMISVLMMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSADELKAWGWRIPFVVGAAAALISLYLRKSLDETSTTASRDKKDAGTIRGVWQHK
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPVFGALSDRIGRRTSMILFGSFAVIG
TVPLMHALKDVTSPVAAFVLITVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSE
ETFYWYVTVLCAISLIVSWRMRDPSKEGYLRHEP
>Mature_434_residues
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQLLNTAGVFAAGFLMRPIGGWL
FGRIADRHGRRNAMMISVLMMCGGSLVIAVLPTYAQIGALAPALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFA
SFQYVTLIGGQLCALLVLVILQQTLSADELKAWGWRIPFVVGAAAALISLYLRKSLDETSTTASRDKKDAGTIRGVWQHK
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPVFGALSDRIGRRTSMILFGSFAVIG
TVPLMHALKDVTSPVAAFVLITVALAIVSFYTSISGLIKAEMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSE
ETFYWYVTVLCAISLIVSWRMRDPSKEGYLRHEP

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=69.4312796208531, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1790550, Length=421, Percent_Identity=30.166270783848, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=28.5057471264368, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1789941, Length=424, Percent_Identity=30.8962264150943, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI87082231, Length=325, Percent_Identity=26.4615384615385, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=312, Percent_Identity=25.3205128205128, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47155; Mature: 47155

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCC
>Mature Secondary Structure
MDVQTDQAALSAHDTRRRVFAIVGASSGNLVEWFDFYIYSFCALYFAPAFFPSGNTTTQL
CCCCCHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LNTAGVFAAGFLMRPIGGWLFGRIADRHGRRNAMMISVLMMCGGSLVIAVLPTYAQIGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
APALLLVARLFQGLSVGGEYGTSATYMSEVALKGRRGFFASFQYVTLIGGQLCALLVLVI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQQTLSADELKAWGWRIPFVVGAAAALISLYLRKSLDETSTTASRDKKDAGTIRGVWQHK
HHHHHCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHCC
GAFFTVVGFTAGGSLIFYTFTTYMQKYLVNTAGMHAKTASNVMTVALLVYMLMQPVFGAL
CCEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SDRIGRRTSMILFGSFAVIGTVPLMHALKDVTSPVAAFVLITVALAIVSFYTSISGLIKA
HHHHCCHHEEHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EMFPPEVRAMGVGLSYAVANAIFGGSAEYVALWFKSVGSEETFYWYVTVLCAISLIVSWR
CCCCCHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
MRDPSKEGYLRHEP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]