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The map label for this gene is selU [H]

Identifier: 78066200

GI number: 78066200

Start: 1722256

End: 1723383

Strand: Direct

Name: selU [H]

Synonym: Bcep18194_A4730

Alternate gene names: 78066200

Gene position: 1722256-1723383 (Clockwise)

Preceding gene: 78066199

Following gene: 78066201

Centisome position: 46.62

GC content: 66.76

Gene sequence:

>1128_bases
TTGAAGAACCTGATTGTCACCCTCGATCGCGCCGGCGAGTTCGACGAGATCATCGACGTGCGCACGCCGCTCGAGTTCGC
CGAGGATCATATTCCGGGCGCGCTGAACGCGCCCGTGCTCAGCAACGAGGAGCGCGTGCTCGTCGGTACGATGTACCGCC
AGGTGTCGCCCTACGAGGCGACGCGCGTCGGCGCGGCCATCGTCGCGCGCAACATCGCCCGCCATCTCGACACGACGTTC
GCCGACCGGCCGCGCAACTGGCGGCCGCTGATCTACTGCTGGCGCGGCGGCAAGCGCTCGGGCTCGATGACGACCTGGTT
CAACCTGATCGGCTGGAAGGCGCGCCAGCTCGACGGCGGCTACAAGGCTTACCGCCAGTCGGTGTGCGCGACGCTCGACT
CGCTGCCGACGCGCTTTCGCTACATCGCGCTCGTCGGCCATACGGGTTGCGGCAAGACGCGCCTGCTGAACGCACTGGGC
GAAGTGGGCGCGCAGACGCTCGATCTCGAGGCGCTTGCGTGCCATCGCGGTTCGCTGCTCGGTGCATTGCCGGGCAAGCC
GCAGCCGGCGCAGAAGGGGTTCGACTCCGGGCTCGTCGAGACACTTGGACGTTTCGACCCCGAATGGCCGGTGTTCGTCG
AGTCGGAAAGCCGCCGGATCGGGCTCGTGCAGTTGCCGATCGCGTTGATCGAAGCGTTTCACGCCGGGCCGTGGGTGCAT
GTCGACGCGGCACACGACGAGCGCATCGCGTTCCTCCTCGACGATTACGCACACCTGTTCGACGAACCGAACGCGTTCAA
GGCACAGCTCCATCGGCTGATCGGACTGCATAGCCGCGAGCAGGTCACGCACTGGAAGGCGCTGATCGATGCGGATGCAC
GGGCCGAACTGTTCACCGAGCTGATCGACAAGCATTACGATCCCGCCTATGCGCGCACCTATCGCGCGACGTACAACAAG
CCGAACCGGGCGCTGACGTTCACGTTCCGCCCCAACGCCGCCGACGGGCGCGATCAGGCGCGTGTGCTGCTGGCCGAACT
CGCGCAAGCCGGGTTGCCCGCATCGGCCGCGCTGGCCGCAGGCGCGAAGCCCGCAACCGACCAAGACCAATCGACGACCA
CACGATGA

Upstream 100 bases:

>100_bases
GCCGCGCGCGGCCCGAGACGCCGGCCCGTGAGATAATCGTCAATCCGCCTTCCGTGCCCATGCGGCTTATCGAGCCGCAT
CGCCCCTGTTCGACGCCAAA

Downstream 100 bases:

>100_bases
CGACCACACGCGCCCAACCCAGTCCGGCCCTCGGCTGGATGACCTTCCTGCTGATTGCGGTCGCGGGCCTGTTCTATGTG
AAATGGTTCCCGTACTACAA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEATRVGAAIVARNIARHLDTTF
ADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGGYKAYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALG
EVGAQTLDLEALACHRGSLLGALPGKPQPAQKGFDSGLVETLGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVH
VDAAHDERIAFLLDDYAHLFDEPNAFKAQLHRLIGLHSREQVTHWKALIDADARAELFTELIDKHYDPAYARTYRATYNK
PNRALTFTFRPNAADGRDQARVLLAELAQAGLPASAALAAGAKPATDQDQSTTTR

Sequences:

>Translated_375_residues
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEATRVGAAIVARNIARHLDTTF
ADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGGYKAYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALG
EVGAQTLDLEALACHRGSLLGALPGKPQPAQKGFDSGLVETLGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVH
VDAAHDERIAFLLDDYAHLFDEPNAFKAQLHRLIGLHSREQVTHWKALIDADARAELFTELIDKHYDPAYARTYRATYNK
PNRALTFTFRPNAADGRDQARVLLAELAQAGLPASAALAAGAKPATDQDQSTTTR
>Mature_375_residues
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEATRVGAAIVARNIARHLDTTF
ADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGGYKAYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALG
EVGAQTLDLEALACHRGSLLGALPGKPQPAQKGFDSGLVETLGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVH
VDAAHDERIAFLLDDYAHLFDEPNAFKAQLHRLIGLHSREQVTHWKALIDADARAELFTELIDKHYDPAYARTYRATYNK
PNRALTFTFRPNAADGRDQARVLLAELAQAGLPASAALAAGAKPATDQDQSTTTR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=352, Percent_Identity=28.4090909090909, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 41620; Mature: 41620

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEA
CCCEEEEECCCCCHHHHHHCCCCHHHHHHCCCCCCCCCEECCCCEEEEEHHHHHCCCHHH
TRVGAAIVARNIARHLDTTFADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHCCCH
YKAYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALGEVGAQTLDLEALACHRGSLL
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEECHHHHHHCCCCEE
GALPGKPQPAQKGFDSGLVETLGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVH
ECCCCCCCHHHCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCEEE
VDAAHDERIAFLLDDYAHLFDEPNAFKAQLHRLIGLHSREQVTHWKALIDADARAELFTE
EECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHH
LIDKHYDPAYARTYRATYNKPNRALTFTFRPNAADGRDQARVLLAELAQAGLPASAALAA
HHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
GAKPATDQDQSTTTR
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLIVTLDRAGEFDEIIDVRTPLEFAEDHIPGALNAPVLSNEERVLVGTMYRQVSPYEA
CCCEEEEECCCCCHHHHHHCCCCHHHHHHCCCCCCCCCEECCCCEEEEEHHHHHCCCHHH
TRVGAAIVARNIARHLDTTFADRPRNWRPLIYCWRGGKRSGSMTTWFNLIGWKARQLDGG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCHHHHCCCH
YKAYRQSVCATLDSLPTRFRYIALVGHTGCGKTRLLNALGEVGAQTLDLEALACHRGSLL
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCEECHHHHHHCCCCEE
GALPGKPQPAQKGFDSGLVETLGRFDPEWPVFVESESRRIGLVQLPIALIEAFHAGPWVH
ECCCCCCCHHHCCCCHHHHHHHHCCCCCCCEEEECCCCEEEEEEHHHHHHHHHCCCCEEE
VDAAHDERIAFLLDDYAHLFDEPNAFKAQLHRLIGLHSREQVTHWKALIDADARAELFTE
EECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHH
LIDKHYDPAYARTYRATYNKPNRALTFTFRPNAADGRDQARVLLAELAQAGLPASAALAA
HHHHCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHC
GAKPATDQDQSTTTR
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]