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The map label for this gene is citH [H]

Identifier: 78066197

GI number: 78066197

Start: 1718328

End: 1719614

Strand: Reverse

Name: citH [H]

Synonym: Bcep18194_A4727

Alternate gene names: 78066197

Gene position: 1719614-1718328 (Counterclockwise)

Preceding gene: 78066198

Following gene: 78066194

Centisome position: 46.55

GC content: 66.2

Gene sequence:

>1287_bases
ATGAATACGACCACCCTGACCTCGCAGGAAGCCGTCCGCCCCAGCGCCGCGAAGATCCGGCGCATCATCTTCGCGGCGTC
GATCGGCAATGCACTCGAATGGTTCGACCTGATCGTCTACGGCTTCTTCGCGGTGACGATCGCCAAGCTGTTCTTCCCCG
CGACGAGCGAAGCGACGTCGCTGATGCTTACGCTCGGCACGTTCGGGCTCTCCTACCTGATCCGGCCGATCGGCGGCTTC
GTGCTCGGCGCATACGCGGATCGCGCGGGCCGCAAGGCGTCGCTGCTACTGTCGATCGCGATGATGATGGCCGGCACGCT
CCTGATCGCGCTGATGCCGACCTACGCATCGATCGGCATACTCGCGCCGCTCGGGATCATGCTGTCGCGGTTGATGCAGG
GCTTTTCCGCGGGCGGTGAATTCGCGAGCTCGACCGCATTCCTCGTCGAGCACGCACCGCAGCGGCGCGGCTTCATGTCG
AGCTGGCAGTTCGCGAGCCAGGGCCTTGCGACGCTGCTCGCATCTGGCTTCGGTGCGTTGCTGACCACCACGCTGACGAC
CGCGCAACTCGAAAGCTGGGGCTGGCGCGTGCCGTTCCTGTTCGGGCTCGCGATCGGCCCGGTCGGGCTGTACATCCGCC
GCTACGTCGACGAAGGCGTCGAGTTCAAGACGCAGGCACGCTCGGAAGCGCCGGTGCGCGAGTTGTTCGCGGAACAAAAG
GTGCGGGTGCTGCTGTCGATCGGCGCGCTCGTGATCTCGACCGCGATCAACTACATGATCCTGTACATGCCGACCTATGC
GATCAAGCAGCTCGGCCTGCCCGCATCGACGGGCTTCGCGGCGACGCTCGCGACCGGCTTCGTGCTCACGCTCGTCACGC
CGGTCGTCGGCCACCTGTCCGATCGCACCGGGCGGATCCGCATGATGGCGGTCGCGGCGGTCCTGATGCTCGTCACCGTG
TACCCGACGTTCGCGTGGCTCACGCGTCACGCATCGTTCGCGACGATGCTCGCCGCCCTGGTCTGGATCGGTGCGCTGAA
AGCGATGTATTGCGGCGCGCTGCCGGCGCTGATGGCCGAGCTGTTCCCGTCGCAGACCCGTGCAACGGGGCTCGCGGTCA
GCTACAACACGGGCGTCACGCTGTTCGGCGGCTTCGCGCCGTTCGTGATCACCTGGCTGATCAGCGCGACCGGCAACCGG
TTGTCGCCGGCGCTCTATCTGATGGGCTGCGCACTGTTGAGCCTCGCCGCGCTGTTCGTCGCGCGCACACGGCTCAAGAT
GCGATAA

Upstream 100 bases:

>100_bases
ATCTCGGCGACGACGCACCGACCGTTCGCCGCGCGGTGCCCGCCGCGGCCGGCGCCGCCCTGTAACGCGCTTCGCCCCTT
TCACGGGACCTGCACATACG

Downstream 100 bases:

>100_bases
CGGAAGAAACGACGGCGCGGCGATGCGCCGCCGGTGCGGCCCGTCGCGGCATCGCTGCCGCGCGGGCCGCGCGTCATTTA
CGGCGCAGGGTTCGGCTGCA

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNTTTLTSQEAVRPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLMLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGILAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTTTLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFAEQK
VRVLLSIGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLSDRTGRIRMMAVAAVLMLVTV
YPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAELFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNR
LSPALYLMGCALLSLAALFVARTRLKMR

Sequences:

>Translated_428_residues
MNTTTLTSQEAVRPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLMLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGILAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTTTLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFAEQK
VRVLLSIGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLSDRTGRIRMMAVAAVLMLVTV
YPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAELFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNR
LSPALYLMGCALLSLAALFVARTRLKMR
>Mature_428_residues
MNTTTLTSQEAVRPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATSLMLTLGTFGLSYLIRPIGGF
VLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGILAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMS
SWQFASQGLATLLASGFGALLTTTLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFAEQK
VRVLLSIGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLSDRTGRIRMMAVAAVLMLVTV
YPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAELFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNR
LSPALYLMGCALLSLAALFVARTRLKMR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=425, Percent_Identity=33.4117647058824, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1788942, Length=401, Percent_Identity=35.4114713216958, Blast_Score=208, Evalue=7e-55,
Organism=Escherichia coli, GI1788292, Length=437, Percent_Identity=29.5194508009153, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=32.093023255814, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI87082231, Length=436, Percent_Identity=23.8532110091743, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87082404, Length=397, Percent_Identity=22.6700251889169, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45937; Mature: 45937

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTTTLTSQEAVRPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATS
CCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LMLTLGTFGLSYLIRPIGGFVLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMSSWQFASQGLATLLASGFGAL
HHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTTTLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFAEQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
VRVLLSIGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DRTGRIRMMAVAAVLMLVTVYPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNRLSPALYLMGCALLSLAALFV
HCCCCHHHCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTRLKMR
HHHHHCCC
>Mature Secondary Structure
MNTTTLTSQEAVRPSAAKIRRIIFAASIGNALEWFDLIVYGFFAVTIAKLFFPATSEATS
CCCCCCCCHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LMLTLGTFGLSYLIRPIGGFVLGAYADRAGRKASLLLSIAMMMAGTLLIALMPTYASIGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAPLGIMLSRLMQGFSAGGEFASSTAFLVEHAPQRRGFMSSWQFASQGLATLLASGFGAL
HHHHHHHHHHHHHHHCCCCCHHCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LTTTLTTAQLESWGWRVPFLFGLAIGPVGLYIRRYVDEGVEFKTQARSEAPVRELFAEQK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHH
VRVLLSIGALVISTAINYMILYMPTYAIKQLGLPASTGFAATLATGFVLTLVTPVVGHLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
DRTGRIRMMAVAAVLMLVTVYPTFAWLTRHASFATMLAALVWIGALKAMYCGALPALMAE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPSQTRATGLAVSYNTGVTLFGGFAPFVITWLISATGNRLSPALYLMGCALLSLAALFV
HCCCCHHHCCEEEEECCCEEEECCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
ARTRLKMR
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]