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The map label for this gene is dusA [H]

Identifier: 78066180

GI number: 78066180

Start: 1702994

End: 1704028

Strand: Direct

Name: dusA [H]

Synonym: Bcep18194_A4710

Alternate gene names: 78066180

Gene position: 1702994-1704028 (Clockwise)

Preceding gene: 78066179

Following gene: 78066182

Centisome position: 46.1

GC content: 64.15

Gene sequence:

>1035_bases
ATGTCCGTACCGTCTTCGCTTCCTCCCCGCCGCGTATCCGTCGCGCCCATGCTCGACTGGACCGATCGCCATTGCCGGTC
GTTCCACCGTACGCTGACGCGCGACACGTGGCTGTATACGGAGATGATCACGACGGGCGCGCTGCTGTTCGGCGATGCCC
AGCGGCATCTCGCGTTCACGCCGAACGAATCGCCGGTCGCGCTTCAACTAGGCGGCAGCGAGCGCGACGATCTCGCGCGC
GCGGCGAAGCTCGGCGAGCAGTGGGGCTACGACGAAATCAACCTGAATTGCGGATGCCCGTCGGAGCGCGTGCAGCGCGG
CGCGTTCGGCGCGTGCCTGATGAACGAGCCGTCGCTCGTCGCCGACTGCGTGAAGGCGATGCGCGACGCGGTGTCGGTGC
CGGTCACGGTCAAGCACCGGATCGGGGTCGACGCGGTCGAAGACTACGCATTCGTGCGCGACTTCGTCGGCACGGTGGCC
GAGGCTGGCTGCGAAACGTTCGTCGTGCATGCGCGCAATGCGATCCTGAAGGGGCTGTCGCCGAAGGAGAATCGCGAGAT
CCCGCCGCTCAAGTACGACTATGCGTATCAGTTGAAGCGCGATTTTCCGTCGCTCGAGATCGTCATCAACGGTGGCATCA
CGACGCTCGACGAGGTTGCGCAGCATCTCGAGCATGTCGACGGCGTGATGCTCGGCCGCGAGGCGTATCACAACCCGTAC
GTGCTCGCGGAGGTTGATGCGCGCTTCTACGGATCGACCGAAGCGGTGCCGACGCGCGAAGAGGCCGAGGCAAAGCTGAT
CGAATACTGCGCGGCCGAACTGAAGCGCGGGACCTACCTCGGCGCGATCGTGCGGCACGCGCTCGGGCTGTATCGCGGCA
TGCCCGGCGCACGCGGTTGGCGTCGTGTGCTGTCCGACAACAAGAAGCTTGCGCGTGCCGATCTGGCCGTGTTCGACGAG
GCGCGCGCGCATCTGAACGAGGCCGAAGAAGTTTTTGCAAAAAAAGCTTTGCAAGATTCAAAAGTGTTCGTATAA

Upstream 100 bases:

>100_bases
GAGCTGGGCGAGGGGTAGCACGCGTGTCGGGCAGGCAGGGCCCGTGTTGCGGTAAAATGCCGGGTTGTTTCCGTGCCGTC
TGGCCTGTTGCCCGAAATCC

Downstream 100 bases:

>100_bases
TCTTGTTCTTCGCTGCTGAAACACAAAAGCAGCGAAGAACAAAAAGCAGTAACAGTGGTGGCTGTAGCTCAGTTGGTAGA
GTCCAGGATTGTGATTCCTG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFTPNESPVALQLGGSERDDLAR
AAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPSLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVA
EAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPSLEIVINGGITTLDEVAQHLEHVDGVMLGREAYHNPY
VLAEVDARFYGSTEAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARGWRRVLSDNKKLARADLAVFDE
ARAHLNEAEEVFAKKALQDSKVFV

Sequences:

>Translated_344_residues
MSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFTPNESPVALQLGGSERDDLAR
AAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPSLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVA
EAGCETFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPSLEIVINGGITTLDEVAQHLEHVDGVMLGREAYHNPY
VLAEVDARFYGSTEAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARGWRRVLSDNKKLARADLAVFDE
ARAHLNEAEEVFAKKALQDSKVFV
>Mature_343_residues
SVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFTPNESPVALQLGGSERDDLARA
AKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPSLVADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVAE
AGCETFVVHARNAILKGLSPKENREIPPLKYDYAYQLKRDFPSLEIVINGGITTLDEVAQHLEHVDGVMLGREAYHNPYV
LAEVDARFYGSTEAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARGWRRVLSDNKKLARADLAVFDEA
RAHLNEAEEVFAKKALQDSKVFV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=236, Percent_Identity=29.6610169491525, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=257, Percent_Identity=27.6264591439689, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=59.9358974358974, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1789660, Length=261, Percent_Identity=24.5210727969349, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788462, Length=188, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=28.7735849056604, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17507177, Length=179, Percent_Identity=29.608938547486, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=26.5560165975104, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24580595, Length=296, Percent_Identity=26.6891891891892, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19920448, Length=296, Percent_Identity=26.6891891891892, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 38237; Mature: 38106

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFT
CCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEC
PNESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPSLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCEECCCCHHH
ADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVAEAGCETFVVHARNAILKGLS
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PKENREIPPLKYDYAYQLKRDFPSLEIVINGGITTLDEVAQHLEHVDGVMLGREAYHNPY
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VLAEVDARFYGSTEAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARGW
EEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
RRVLSDNKKLARADLAVFDEARAHLNEAEEVFAKKALQDSKVFV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVPSSLPPRRVSVAPMLDWTDRHCRSFHRTLTRDTWLYTEMITTGALLFGDAQRHLAFT
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEEEC
PNESPVALQLGGSERDDLARAAKLGEQWGYDEINLNCGCPSERVQRGAFGACLMNEPSLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCCCCEECCCCHHH
ADCVKAMRDAVSVPVTVKHRIGVDAVEDYAFVRDFVGTVAEAGCETFVVHARNAILKGLS
HHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCC
PKENREIPPLKYDYAYQLKRDFPSLEIVINGGITTLDEVAQHLEHVDGVMLGREAYHNPY
CCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VLAEVDARFYGSTEAVPTREEAEAKLIEYCAAELKRGTYLGAIVRHALGLYRGMPGARGW
EEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHH
RRVLSDNKKLARADLAVFDEARAHLNEAEEVFAKKALQDSKVFV
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]