BacMap

Definition	Burkholderia sp. 383 chromosome 1, complete genome.
Accession	NC_007510
Length	3,694,126

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The map label for this gene is braG [H]

Identifier: 78066032

GI number: 78066032

Start: 1533931

End: 1534635

Strand: Direct

Name: braG [H]

Synonym: Bcep18194_A4561

Alternate gene names: 78066032

Gene position: 1533931-1534635 (Clockwise)

Preceding gene: 78066031

Following gene: 78066033

Centisome position: 41.52

GC content: 73.33

Gene sequence:

>705_bases
ATGAGCGCGTTGCTCGACATTCGCGACCTGCGCGCATGGTACGGGCTGCAACCCGTGCTCGACGGCGTCGATCTCGCGCT
GGCGCCCGGCGAGACGCTCGCGCTGCTCGGCCGCAACGGTTCGGGGCGCTCGACGCTCGCGAAGGCCGTGATGGGGCTCG
TGCGCACGGCAGGCTCGGTGCGCATCGGCGGCGCCGAATGCGTGGGCGCACGCACGTTCGAGATCGCGCGGCGCGGCGTG
GCTTACGTTGCCGAAAGCCGCGACGTATTCCCGCTGCTGACGGTGCGTGACAACCTGCGGCTCGGGCTGCGCGGCGCCAG
CGGCGCGGCCGAGCACACGGCGCTCGAACGGCTGTTCGACCGCTTTCCGTTGCTGGCCGCGCGCGCGGACGTGAAGGCGG
GGCGGCTGTCGGGCGGCGAGCAGCAGGTGCTTGCGCTGGTGCGGGCGCTGGCCGGCCGCCCGCGCGTGCTGATCGTCGAT
GAACCGGCCGAAGGGCTCGCGCCGCTAGCCGTCGACGAAGTCGGCGCATGCCTCGCCGCGCTGCAGGCCGACGGTGTCGC
GATCGTGCTGATCGAGCAGCGGCTGCAGTTCGCGCCAAGGCTCGCGCGGCGCGTCGCGGTCATGGGGCGCGGACGGATCG
TCTACGACGGTGCACTCGACGGCCTGGGCGGCGGTGTCGCGAACGCATGGCTGAGCGCCGGCTGA

Upstream 100 bases:

>100_bases
TCACGGTGCTCGTGCGCGGCACGGTGGTCGCGACCGGCGCGCCCGATGCGATCCGCACCGATCCGGCCGTGCGTGCCGCC
TATCTCGGCGAGGGCGGCCC

Downstream 100 bases:

>100_bases
CGGCACGCGTGCGATTGTTCGGAAAACTCCGCCAGTCAATTCGTGTCGAGCCGCTTTATCGCCGCCGCAGCGCACAGTAA
ATTTCCAGCCATACCGGTTG

Product: branched chain amino acid ABC transporter ATPase

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSALLDIRDLRAWYGLQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSVRIGGAECVGARTFEIARRGV
AYVAESRDVFPLLTVRDNLRLGLRGASGAAEHTALERLFDRFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVGACLAALQADGVAIVLIEQRLQFAPRLARRVAVMGRGRIVYDGALDGLGGGVANAWLSAG

Sequences:

>Translated_234_residues
MSALLDIRDLRAWYGLQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSVRIGGAECVGARTFEIARRGV
AYVAESRDVFPLLTVRDNLRLGLRGASGAAEHTALERLFDRFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVD
EPAEGLAPLAVDEVGACLAALQADGVAIVLIEQRLQFAPRLARRVAVMGRGRIVYDGALDGLGGGVANAWLSAG
>Mature_233_residues
SALLDIRDLRAWYGLQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSVRIGGAECVGARTFEIARRGVA
YVAESRDVFPLLTVRDNLRLGLRGASGAAEHTALERLFDRFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVDE
PAEGLAPLAVDEVGACLAALQADGVAIVLIEQRLQFAPRLARRVAVMGRGRIVYDGALDGLGGGVANAWLSAG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=215, Percent_Identity=29.3023255813954, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI150417984, Length=233, Percent_Identity=33.0472103004292, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI47078218, Length=218, Percent_Identity=28.8990825688073, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI45446740, Length=218, Percent_Identity=28.8990825688073, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27436953, Length=204, Percent_Identity=28.4313725490196, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI90421313, Length=199, Percent_Identity=27.6381909547739, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI27477115, Length=202, Percent_Identity=30.1980198019802, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI105990541, Length=204, Percent_Identity=25.9803921568627, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI6005701, Length=214, Percent_Identity=28.9719626168224, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI21536376, Length=227, Percent_Identity=26.431718061674, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI46592898, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592964, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI46592915, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI46592971, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI46592978, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI46592956, Length=233, Percent_Identity=25.7510729613734, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI31657092, Length=194, Percent_Identity=28.8659793814433, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87082268, Length=222, Percent_Identity=35.1351351351351, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789672, Length=231, Percent_Identity=31.1688311688312, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788225, Length=232, Percent_Identity=33.1896551724138, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787029, Length=219, Percent_Identity=30.5936073059361, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787792, Length=223, Percent_Identity=30.9417040358744, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786872, Length=226, Percent_Identity=31.4159292035398, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87081791, Length=221, Percent_Identity=31.6742081447964, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787089, Length=223, Percent_Identity=30.4932735426009, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790190, Length=208, Percent_Identity=31.7307692307692, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789032, Length=214, Percent_Identity=34.5794392523364, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081782, Length=230, Percent_Identity=29.1304347826087, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1790544, Length=238, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1787370, Length=223, Percent_Identity=31.390134529148, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1789991, Length=222, Percent_Identity=26.5765765765766, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788761, Length=226, Percent_Identity=31.4159292035398, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788897, Length=221, Percent_Identity=28.9592760180996, Blast_Score=85, Evalue=3e-18,
Organism=Escherichia coli, GI1786703, Length=192, Percent_Identity=34.8958333333333, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787712, Length=217, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786253, Length=239, Percent_Identity=28.8702928870293, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789593, Length=220, Percent_Identity=27.7272727272727, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790162, Length=233, Percent_Identity=25.3218884120172, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786563, Length=213, Percent_Identity=30.9859154929577, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786319, Length=227, Percent_Identity=28.6343612334802, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788472, Length=210, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789864, Length=236, Percent_Identity=27.5423728813559, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790525, Length=213, Percent_Identity=29.5774647887324, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1789891, Length=210, Percent_Identity=34.2857142857143, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081834, Length=211, Percent_Identity=31.2796208530806, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1788450, Length=218, Percent_Identity=28.4403669724771, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48995001, Length=200, Percent_Identity=29.5, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081709, Length=175, Percent_Identity=30.2857142857143, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787500, Length=247, Percent_Identity=27.1255060728745, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI48994943, Length=217, Percent_Identity=28.110599078341, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1789873, Length=208, Percent_Identity=27.4038461538462, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1789963, Length=235, Percent_Identity=28.936170212766, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI145693107, Length=210, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788644, Length=237, Percent_Identity=27.8481012658228, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1786398, Length=201, Percent_Identity=29.3532338308458, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788210, Length=200, Percent_Identity=27.5, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788165, Length=177, Percent_Identity=32.2033898305085, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787164, Length=209, Percent_Identity=33.0143540669856, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787105, Length=231, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994883, Length=241, Percent_Identity=27.3858921161826, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787547, Length=233, Percent_Identity=25.7510729613734, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1786698, Length=161, Percent_Identity=31.6770186335404, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788506, Length=234, Percent_Identity=30.7692307692308, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082267, Length=159, Percent_Identity=30.8176100628931, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790467, Length=159, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786975, Length=208, Percent_Identity=26.4423076923077, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787113, Length=201, Percent_Identity=30.8457711442786, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787112, Length=223, Percent_Identity=30.0448430493274, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=236, Percent_Identity=30.9322033898305, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71983523, Length=201, Percent_Identity=31.8407960199005, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983516, Length=201, Percent_Identity=31.8407960199005, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=201, Percent_Identity=31.8407960199005, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=219, Percent_Identity=27.8538812785388, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI193209708, Length=211, Percent_Identity=28.9099526066351, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=204, Percent_Identity=27.9411764705882, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193208177, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17543740, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=214, Percent_Identity=28.9719626168224, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17567265, Length=158, Percent_Identity=29.746835443038, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71992290, Length=208, Percent_Identity=27.4038461538462, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17565938, Length=212, Percent_Identity=29.7169811320755, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=234, Percent_Identity=24.7863247863248, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=216, Percent_Identity=27.7777777777778, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17569143, Length=214, Percent_Identity=27.5700934579439, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193211017, Length=228, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193211015, Length=228, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71996809, Length=228, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17541710, Length=196, Percent_Identity=28.0612244897959, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17567269, Length=163, Percent_Identity=33.1288343558282, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI212646699, Length=212, Percent_Identity=27.8301886792453, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24641565, Length=222, Percent_Identity=31.0810810810811, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24666092, Length=221, Percent_Identity=28.9592760180996, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI116007184, Length=195, Percent_Identity=29.2307692307692, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221500365, Length=195, Percent_Identity=29.2307692307692, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574150, Length=222, Percent_Identity=26.5765765765766, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007328, Length=222, Percent_Identity=26.5765765765766, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581191, Length=211, Percent_Identity=27.4881516587678, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581189, Length=211, Percent_Identity=27.4881516587678, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581193, Length=211, Percent_Identity=27.4881516587678, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581195, Length=211, Percent_Identity=27.4881516587678, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643674, Length=220, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648314, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI221512771, Length=216, Percent_Identity=27.3148148148148, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574533, Length=215, Percent_Identity=27.4418604651163, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62484234, Length=210, Percent_Identity=25.7142857142857, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397593, Length=218, Percent_Identity=28.8990825688073, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 24408; Mature: 24276

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLDIRDLRAWYGLQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSV
CCCHHHHHHHHHHHCCCHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCE
RIGGAECVGARTFEIARRGVAYVAESRDVFPLLTVRDNLRLGLRGASGAAEHTALERLFD
EECCHHHCCHHHHHHHHHHHHHEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHH
RFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVDEPAEGLAPLAVDEVGACLAA
HCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LQADGVAIVLIEQRLQFAPRLARRVAVMGRGRIVYDGALDGLGGGVANAWLSAG
HHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SALLDIRDLRAWYGLQPVLDGVDLALAPGETLALLGRNGSGRSTLAKAVMGLVRTAGSV
CCHHHHHHHHHHHCCCHHHCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCE
RIGGAECVGARTFEIARRGVAYVAESRDVFPLLTVRDNLRLGLRGASGAAEHTALERLFD
EECCHHHCCHHHHHHHHHHHHHEECCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHHHH
RFPLLAARADVKAGRLSGGEQQVLALVRALAGRPRVLIVDEPAEGLAPLAVDEVGACLAA
HCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHH
LQADGVAIVLIEQRLQFAPRLARRVAVMGRGRIVYDGALDGLGGGVANAWLSAG
HHCCCEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]