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The map label for this gene is braC [H]

Identifier: 78065940

GI number: 78065940

Start: 1430692

End: 1431906

Strand: Direct

Name: braC [H]

Synonym: Bcep18194_A4469

Alternate gene names: 78065940

Gene position: 1430692-1431906 (Clockwise)

Preceding gene: 78065937

Following gene: 78065941

Centisome position: 38.73

GC content: 65.6

Gene sequence:

>1215_bases
ATGCAGTCGCCCGGTCGGGAATCATGGCGGCTGCAGCGTTCGTTCACTTCGATCACGGAGCCGCCAGTGAAGCCTATCTC
TCCCCGCATGCCGCTCGTCGGCGCAACCGTCGTGCTGGTCGCGGCAAGCATGCAGGCCCGCGCGGACACTCCATCCGTCG
TGCTCATCGGGCATGCCGCGCCGCTCACCGGGCAACTGGCGAACATGGGCAAGGACAGCGAGAATGCCGCGCGTCTTGCC
ATCGAGGAAATCAACAGCCGCCAGCCGGTCGTCGGCGGCAAACCGGTGCGCTTCCAGCTCGATTCGCAGGACGACGCGGC
CGATCCGCGTACCGGCACGCAGGTCGCGCAGAAGCTGGTGGACGATGGCGTCGTCGCGGTGGTCGGCGACATCAATTCGG
GCGTGTCCATTCCGGCGTCACGGATCTACAACGACGCGCACGTCACGCAGATATCGCAGGGTTCGACGAATCCGGTGTTC
ACGCAGCAGGGGTATCGGACGACCTATCGCCTGGTCGCGACGGACGCGATGCAGGGGCCGGCGCTGGCGCGATACGCCAT
CGCGACGCTCGGGGCAAAGCGCGTGGCCGTCATCGACGATTCGACCGCGTACGGCCAGGGGCTCGCGAACGAATTCGAGA
AGGCGGCAAAGGCGTCGGGGGCCCAGGTCGTCACGCGAGAGGCAACGAACGACAAGGCGATCGACTTTCGCGCGATCCTC
ACCAAGATCAAGGGCCTGCAGCCCGACGTGATCATGTACGGCGGCTCGGACGCGACGGCCGGCCCGCTGGTGAAGCAGGC
GGCGAATCTGGGGATTCGGGCGCGCGTGCTGGGCGGGGACGGGGCCTGCACCGACAAGATGATCGGGCTGGCGGGCGACG
CGATCGACAACCTGGTCTGTTCGGAGGCCGGCCTTGCATTGTCGAAGATGCCGAAAGGGCAGGATTTCAACAATCGGTAC
GTGGCCCGCTACAACGCGCAGATCGATGCGTACGCGCCGTTTGCGTACGATGCCGTGTACGTCATCTACGATGCGATCAA
GCGTGCCGGGTCGACGGATCGGGCGAAAGTGCTCGCGGCGATGCCCGCCACGCAGTATGACGGCGTGACCGGGAGGATCG
CGTTCGATTCGCGCGGCGACCTGAAGAATGCCGCGATCACGATCTATCGCTTCAAGGACCACAAGAAGGCCGTCGCGGAC
GTGGTCCGGATGTAA

Upstream 100 bases:

>100_bases
CCGGGCCGCGCGTGCCATGCGCCGCGATAAGTTTTATTTGTCGGCGGCGAACGGAATTTGTCGTTGGACGCGGAATTTCG
GCGATTGAACACTGGGTCGC

Downstream 100 bases:

>100_bases
CGAAGCATCGCCGCGAACCCGTCGCGCATCGATTCGCGGGACTTCCCGTCCGGGCGCGACGCTCGACCCCCGTGGGCACG
CACGCCGTCCCTCGTGGGTG

Product: branched chain amino acid ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MQSPGRESWRLQRSFTSITEPPVKPISPRMPLVGATVVLVAASMQARADTPSVVLIGHAAPLTGQLANMGKDSENAARLA
IEEINSRQPVVGGKPVRFQLDSQDDAADPRTGTQVAQKLVDDGVVAVVGDINSGVSIPASRIYNDAHVTQISQGSTNPVF
TQQGYRTTYRLVATDAMQGPALARYAIATLGAKRVAVIDDSTAYGQGLANEFEKAAKASGAQVVTREATNDKAIDFRAIL
TKIKGLQPDVIMYGGSDATAGPLVKQAANLGIRARVLGGDGACTDKMIGLAGDAIDNLVCSEAGLALSKMPKGQDFNNRY
VARYNAQIDAYAPFAYDAVYVIYDAIKRAGSTDRAKVLAAMPATQYDGVTGRIAFDSRGDLKNAAITIYRFKDHKKAVAD
VVRM

Sequences:

>Translated_404_residues
MQSPGRESWRLQRSFTSITEPPVKPISPRMPLVGATVVLVAASMQARADTPSVVLIGHAAPLTGQLANMGKDSENAARLA
IEEINSRQPVVGGKPVRFQLDSQDDAADPRTGTQVAQKLVDDGVVAVVGDINSGVSIPASRIYNDAHVTQISQGSTNPVF
TQQGYRTTYRLVATDAMQGPALARYAIATLGAKRVAVIDDSTAYGQGLANEFEKAAKASGAQVVTREATNDKAIDFRAIL
TKIKGLQPDVIMYGGSDATAGPLVKQAANLGIRARVLGGDGACTDKMIGLAGDAIDNLVCSEAGLALSKMPKGQDFNNRY
VARYNAQIDAYAPFAYDAVYVIYDAIKRAGSTDRAKVLAAMPATQYDGVTGRIAFDSRGDLKNAAITIYRFKDHKKAVAD
VVRM
>Mature_404_residues
MQSPGRESWRLQRSFTSITEPPVKPISPRMPLVGATVVLVAASMQARADTPSVVLIGHAAPLTGQLANMGKDSENAARLA
IEEINSRQPVVGGKPVRFQLDSQDDAADPRTGTQVAQKLVDDGVVAVVGDINSGVSIPASRIYNDAHVTQISQGSTNPVF
TQQGYRTTYRLVATDAMQGPALARYAIATLGAKRVAVIDDSTAYGQGLANEFEKAAKASGAQVVTREATNDKAIDFRAIL
TKIKGLQPDVIMYGGSDATAGPLVKQAANLGIRARVLGGDGACTDKMIGLAGDAIDNLVCSEAGLALSKMPKGQDFNNRY
VARYNAQIDAYAPFAYDAVYVIYDAIKRAGSTDRAKVLAAMPATQYDGVTGRIAFDSRGDLKNAAITIYRFKDHKKAVAD
VVRM

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=364, Percent_Identity=31.5934065934066, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI48994941, Length=357, Percent_Identity=26.6106442577031, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42849; Mature: 42849

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSPGRESWRLQRSFTSITEPPVKPISPRMPLVGATVVLVAASMQARADTPSVVLIGHAA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PLTGQLANMGKDSENAARLAIEEINSRQPVVGGKPVRFQLDSQDDAADPRTGTQVAQKLV
CCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
DDGVVAVVGDINSGVSIPASRIYNDAHVTQISQGSTNPVFTQQGYRTTYRLVATDAMQGP
CCCEEEEEECCCCCCCCCHHHHCCCHHHEEECCCCCCCCEECCCCCEEEEEEEECCCCCC
ALARYAIATLGAKRVAVIDDSTAYGQGLANEFEKAAKASGAQVVTREATNDKAIDFRAIL
HHHHHHHHHCCCCEEEEEECCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
TKIKGLQPDVIMYGGSDATAGPLVKQAANLGIRARVLGGDGACTDKMIGLAGDAIDNLVC
HHHCCCCCCEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCHHHHHHHHH
SEAGLALSKMPKGQDFNNRYVARYNAQIDAYAPFAYDAVYVIYDAIKRAGSTDRAKVLAA
HHCCCHHHCCCCCCCCCCCEEEEEECEEEEECCHHHHHHHHHHHHHHHCCCCCHHHEEEE
MPATQYDGVTGRIAFDSRGDLKNAAITIYRFKDHKKAVADVVRM
CCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MQSPGRESWRLQRSFTSITEPPVKPISPRMPLVGATVVLVAASMQARADTPSVVLIGHAA
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCC
PLTGQLANMGKDSENAARLAIEEINSRQPVVGGKPVRFQLDSQDDAADPRTGTQVAQKLV
CCCHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHH
DDGVVAVVGDINSGVSIPASRIYNDAHVTQISQGSTNPVFTQQGYRTTYRLVATDAMQGP
CCCEEEEEECCCCCCCCCHHHHCCCHHHEEECCCCCCCCEECCCCCEEEEEEEECCCCCC
ALARYAIATLGAKRVAVIDDSTAYGQGLANEFEKAAKASGAQVVTREATNDKAIDFRAIL
HHHHHHHHHCCCCEEEEEECCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
TKIKGLQPDVIMYGGSDATAGPLVKQAANLGIRARVLGGDGACTDKMIGLAGDAIDNLVC
HHHCCCCCCEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCHHHHHHHHH
SEAGLALSKMPKGQDFNNRYVARYNAQIDAYAPFAYDAVYVIYDAIKRAGSTDRAKVLAA
HHCCCHHHCCCCCCCCCCCEEEEEECEEEEECCHHHHHHHHHHHHHHHCCCCCHHHEEEE
MPATQYDGVTGRIAFDSRGDLKNAAITIYRFKDHKKAVADVVRM
CCCCCCCCCEEEEEECCCCCCCCEEEEEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]