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The map label for this gene is proP [H]

Identifier: 78065895

GI number: 78065895

Start: 1381000

End: 1382487

Strand: Reverse

Name: proP [H]

Synonym: Bcep18194_A4424

Alternate gene names: 78065895

Gene position: 1382487-1381000 (Counterclockwise)

Preceding gene: 78065897

Following gene: 78065892

Centisome position: 37.42

GC content: 67.61

Gene sequence:

>1488_bases
ATGACCTTGACCGCAACACACGTCAGCCCGACCGCTGCTTCTTCCTCCGCTTCGTCGTCCGGCGAAGCCCCGCTCGTCGC
GGACGACATAACCGTCGTCGACCAGAGCCTACTCAAGCGCGCCGTCAGCGCGATGGCCATCGGCAACGCGATGGAATGGT
TCGACTTCGGCGTCTACAGCTACATCGCCGTCACGCTCGGCAAGGTGTTCTTCCCGTCCAGCAGCCCGTCCGCGCAGCTG
CTCGCGACCTTCGGCACGTTCGCGGCCGCGTTCCTCGTGCGCCCGCTCGGCGGCATGGTGTTCGGCCCGCTCGGCGACCG
CATCGGCCGCCAGCGCGTGCTCGCCGCCACGATGATCATGATGGCCGTCGGCACCTTCGCGATCGGCCTGATCCCCAGCT
ACGCGTCGATCGGCATCATGGCGCCCGTGCTGCTGCTCGTCGCCCGCCTCGTGCAGGGTTTCTCGACCGGCGGCGAATAC
GGCGGCGCAGCCACCTTCATCGCCGAATTCTCGACCGACAAGCGCCGCGGCTTCATGGGCAGCTTCCTCGAATTCGGCAC
GCTGATCGGTTACGTGATGGGTGCGGGCGTCGTCGCGCTGCTCACCGCGTCGCTGTCGCAGGAGGCGCTGCTGTCGTGGG
GCTGGCGCGTGCCGTTCCTGATCGCCGGCCCGCTCGGCCTGATCGGTCTCTACATCCGGATGAAGCTCGAGGAAACGCCT
GCGTTCAAGCGCCAGGCCGAAGAGCGCGAAGCGCAGGACAAGGCCGTGCCGAAGACGCGCTTCCGCGAAACGCTGATGCG
CAACTGGCGTGCCCTGCTGCTGTGCGTCGGCCTCGTGCTGATCTTCAACGTGACCGACTACATGGTGCTGTCGTACCTGC
CGAGCTTCATGTCGTCGACGCTGCACTTCGACGAATCGCACAGCCTCGTGCTCGTGCTGATCGTGATGGTGCTGATGATG
CCGCTGACGCTCGCCGCCGGCCGCCTGTCCGACCGGATCGGCCGCAAGCCCGTGATGCTCGCCGGCTGCGTCGGCCTGCT
CGCGCTGTCGATCCCGTCGATGATGCTGATCCATGCGGGCACCACGGCGTCGGTGTTCGGCGGCCTGCTGATCCTCGGCG
TGCTGCTGTCGTGCTTCACCGGCGTGATGCCGTCGGCGCTGCCGGCGCTGTTCCCGACCGAGATCCGCTACGGCGCGCTC
GCGATCGGCTTCAACGTGTCGGTGTCGCTGTTCGGCGGCACGACGCCGCTCGTGACCGCGTGGCTGGTCGACGTCACCCA
CAACCTGATGATGCCCGCGTACTACATGATGGGCGCCGCCGTGATCGGCATCGTGTCGGTCGTCGCGCTCGCCGAAACCG
CGCGTCAGCCGCTCAAGGGTTCGCCGCCGGCCGTCGCGTCGCGTCGCGAAGCCCATCAGCTCGTGCGCCAGATGCGCGAG
GACGACGAATCGGAGATGTACGGTGTCGTATCGCCGGCACGCGCCTGA

Upstream 100 bases:

>100_bases
TAAGTGCTTAAAGGTTTGCCCCGACGCGCCGCACCCGACACCGCGCTCGCCGGCAAACCGTGTCGGACGGCCCGCCCGTT
CGGCGCAGTCAAAGGAGTGC

Downstream 100 bases:

>100_bases
GCGTCGCCCATGAAAAAGCCCCGGCCGAAGCCGGGGCCCGATCGAACCCGATCCGCCCGACCGGCTTGCGCCGGCCGGGC
TTTTTTCATCCATGCGACGC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTLTATHVSPTAASSSASSSGEAPLVADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQL
LATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGTFAIGLIPSYASIGIMAPVLLLVARLVQGFSTGGEY
GGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
AFKRQAEEREAQDKAVPKTRFRETLMRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMM
PLTLAAGRLSDRIGRKPVMLAGCVGLLALSIPSMMLIHAGTTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGAL
AIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALAETARQPLKGSPPAVASRREAHQLVRQMRE
DDESEMYGVVSPARA

Sequences:

>Translated_495_residues
MTLTATHVSPTAASSSASSSGEAPLVADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQL
LATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGTFAIGLIPSYASIGIMAPVLLLVARLVQGFSTGGEY
GGAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
AFKRQAEEREAQDKAVPKTRFRETLMRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMM
PLTLAAGRLSDRIGRKPVMLAGCVGLLALSIPSMMLIHAGTTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGAL
AIGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALAETARQPLKGSPPAVASRREAHQLVRQMRE
DDESEMYGVVSPARA
>Mature_494_residues
TLTATHVSPTAASSSASSSGEAPLVADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYSYIAVTLGKVFFPSSSPSAQLL
ATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIMMAVGTFAIGLIPSYASIGIMAPVLLLVARLVQGFSTGGEYG
GAATFIAEFSTDKRRGFMGSFLEFGTLIGYVMGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETPA
FKRQAEEREAQDKAVPKTRFRETLMRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSSTLHFDESHSLVLVLIVMVLMMP
LTLAAGRLSDRIGRKPVMLAGCVGLLALSIPSMMLIHAGTTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALA
IGFNVSVSLFGGTTPLVTAWLVDVTHNLMMPAYYMMGAAVIGIVSVVALAETARQPLKGSPPAVASRREAHQLVRQMRED
DESEMYGVVSPARA

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=467, Percent_Identity=50.321199143469, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=31.322505800464, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI1788942, Length=405, Percent_Identity=34.5679012345679, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=34.032634032634, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87082231, Length=430, Percent_Identity=23.2558139534884, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082404, Length=365, Percent_Identity=23.2876712328767, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 52721; Mature: 52590

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTATHVSPTAASSSASSSGEAPLVADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYS
CCEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
YIAVTLGKVFFPSSSPSAQLLATFGTFAAAFLVRPLGGMVFGPLGDRIGRQRVLAATMIM
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHH
MAVGTFAIGLIPSYASIGIMAPVLLLVARLVQGFSTGGEYGGAATFIAEFSTDKRRGFMG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHH
SFLEFGTLIGYVMGAGVVALLTASLSQEALLSWGWRVPFLIAGPLGLIGLYIRMKLEETP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHEECCCCC
AFKRQAEEREAQDKAVPKTRFRETLMRNWRALLLCVGLVLIFNVTDYMVLSYLPSFMSST
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHFDESHSLVLVLIVMVLMMPLTLAAGRLSDRIGRKPVMLAGCVGLLALSIPSMMLIHAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCC
TTASVFGGLLILGVLLSCFTGVMPSALPALFPTEIRYGALAIGFNVSVSLFGGTTPLVTA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCEEEEECEEEEEEECCCHHHHHH
WLVDVTHNLMMPAYYMMGAAVIGIVSVVALAETARQPLKGSPPAVASRREAHQLVRQMRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
DDESEMYGVVSPARA
CCHHHHEECCCCCCC
>Mature Secondary Structure 
TLTATHVSPTAASSSASSSGEAPLVADDITVVDQSLLKRAVSAMAIGNAMEWFDFGVYS
CEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH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CCHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]