Click here to switch to the map view.

The map label for this gene is miaA

Identifier: 78065326

GI number: 78065326

Start: 758934

End: 759908

Strand: Reverse

Name: miaA

Synonym: Bcep18194_A3852

Alternate gene names: 78065326

Gene position: 759908-758934 (Counterclockwise)

Preceding gene: 78065327

Following gene: 78065324

Centisome position: 20.57

GC content: 70.67

Gene sequence:

>975_bases
ATGAGCGCGGCACCGCACGCCAGCCCGACGACGATCGCCTGCCTGCTCGGCCCCACCGCATCGGGCAAGACGGCCGCGGC
GCTGGCGCTCGCCGCGCGCCGCCCGATCGAGATCGTCAGCGTCGATTCGGCGCTCGTCTACCGCGACATGGATATCGGCA
CCGCGAAGCCTTCGCGCGACGAGCGCGCGAGCGTGCCGCATCACCTGATCGACATCATCGATCCGGCCGACGCGTATTCG
GCCGCGAGCTTCCGCGCGGACACGCTGCGCCTGATCGGCGAGATCGCCGCGCGTGGCCGCACACCGCTGCTCGCGGGCGG
CACGATGCTGTACTACAAGGCGCTGACGCAGGGGCTCAACGACCTGCCAGGCGCCGATCCGGATGTGCGCGCGACACTCG
ATGCCGAAGCCGAGCGTGACGGCTGGCCCGCGCTGCACGCGCGGCTCGCGCAAGTCGATCCCGACACGGCCGCACGGCTC
GCGCCGAACGACTCGCAGCGGATCCAGCGGGCGCTCGAAGTGTTCATGCTGAGCGGGCAGCCGATGTCGGTACTGCTCGC
CGCGCCGCGCCGCACGGACGATGCGGCCGCCGCTTACCGCTTCGTGCCGGTCGCGCTCGAACCGTCGGATCGCGCGGTAC
TGCACAAACGGATCGCACAGCGCTTCGACGCGATGCTAGACGCAGGCTTCATCGACGAAGTCGAGCGACTGCGCCGACGC
GAGGACCTCCATCCCGACCTGCCGTCGATGCGCTGCGTCGGCTACCGGCAGGCGTGGGAATTCCTCGACGGCGACACCGA
CTACCGGACGATGCGCGACAAGGGCATCTTCGCGACGCGCCAGCTCTGCAAGCGCCAGATCACCTGGCTGCGCGCGATGC
CGGAGCGGATCGTCGTCGACTGCATCGCACCGGACTCGACGGCCCGCGCACTCGACGCGCTCGAACGCGTGCTCGACGGC
AGCACGCCGGGCTGA

Upstream 100 bases:

>100_bases
AGATGGAGGCGACCGAACGCGCGGACCAGTGCAATCACGGCCGGCCGACGTGGTATCAGCTCACGCTGAACGATCTCGAC
CGCCTCTTCATGCGCGGCCA

Downstream 100 bases:

>100_bases
CCGCGTCAGCCGCGCTCGCGCGCTGCCAGGCGCGCGCGGGCGCCGTCTACGATCCACGCGACGCCGCGCTCGAACGCGTC
GTCGCCGCTGCGCCCGCGCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSAAPHASPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRDERASVPHHLIDIIDPADAYS
AASFRADTLRLIGEIAARGRTPLLAGGTMLYYKALTQGLNDLPGADPDVRATLDAEAERDGWPALHARLAQVDPDTAARL
APNDSQRIQRALEVFMLSGQPMSVLLAAPRRTDDAAAAYRFVPVALEPSDRAVLHKRIAQRFDAMLDAGFIDEVERLRRR
EDLHPDLPSMRCVGYRQAWEFLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDSTARALDALERVLDG
STPG

Sequences:

>Translated_324_residues
MSAAPHASPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRDERASVPHHLIDIIDPADAYS
AASFRADTLRLIGEIAARGRTPLLAGGTMLYYKALTQGLNDLPGADPDVRATLDAEAERDGWPALHARLAQVDPDTAARL
APNDSQRIQRALEVFMLSGQPMSVLLAAPRRTDDAAAAYRFVPVALEPSDRAVLHKRIAQRFDAMLDAGFIDEVERLRRR
EDLHPDLPSMRCVGYRQAWEFLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDSTARALDALERVLDG
STPG
>Mature_323_residues
SAAPHASPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRDERASVPHHLIDIIDPADAYSA
ASFRADTLRLIGEIAARGRTPLLAGGTMLYYKALTQGLNDLPGADPDVRATLDAEAERDGWPALHARLAQVDPDTAARLA
PNDSQRIQRALEVFMLSGQPMSVLLAAPRRTDDAAAAYRFVPVALEPSDRAVLHKRIAQRFDAMLDAGFIDEVERLRRRE
DLHPDLPSMRCVGYRQAWEFLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVDCIAPDSTARALDALERVLDGS
TPG

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=310, Percent_Identity=31.9354838709677, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=51.6339869281046, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI25144712, Length=302, Percent_Identity=27.1523178807947, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324848, Length=294, Percent_Identity=27.2108843537415, Blast_Score=131, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24649830, Length=286, Percent_Identity=30.7692307692308, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35404; Mature: 35273

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAPHASPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRD
CCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCC
ERASVPHHLIDIIDPADAYSAASFRADTLRLIGEIAARGRTPLLAGGTMLYYKALTQGLN
CHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
DLPGADPDVRATLDAEAERDGWPALHARLAQVDPDTAARLAPNDSQRIQRALEVFMLSGQ
HCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
PMSVLLAAPRRTDDAAAAYRFVPVALEPSDRAVLHKRIAQRFDAMLDAGFIDEVERLRRR
CCEEEEECCCCCCHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EDLHPDLPSMRCVGYRQAWEFLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVD
HCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHEEE
CIAPDSTARALDALERVLDGSTPG
EECCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAAPHASPTTIACLLGPTASGKTAAALALAARRPIEIVSVDSALVYRDMDIGTAKPSRD
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCCCCCC
ERASVPHHLIDIIDPADAYSAASFRADTLRLIGEIAARGRTPLLAGGTMLYYKALTQGLN
CHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHH
DLPGADPDVRATLDAEAERDGWPALHARLAQVDPDTAARLAPNDSQRIQRALEVFMLSGQ
HCCCCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCC
PMSVLLAAPRRTDDAAAAYRFVPVALEPSDRAVLHKRIAQRFDAMLDAGFIDEVERLRRR
CCEEEEECCCCCCHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EDLHPDLPSMRCVGYRQAWEFLDGDTDYRTMRDKGIFATRQLCKRQITWLRAMPERIVVD
HCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHCHHHHEEE
CIAPDSTARALDALERVLDGSTPG
EECCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA