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The map label for this gene is purB [H]

Identifier: 78065228

GI number: 78065228

Start: 643837

End: 645285

Strand: Reverse

Name: purB [H]

Synonym: Bcep18194_A3752

Alternate gene names: 78065228

Gene position: 645285-643837 (Counterclockwise)

Preceding gene: 78065235

Following gene: 78065226

Centisome position: 17.47

GC content: 65.36

Gene sequence:

>1449_bases
ATGGTAAAATGCCGGATTCTCCTTCCCCTCGCTCGACCCGGCCCCAAGGCCCGCCCGATCATGTCCGACACTCGTCCCGA
TACGCTTTTCGCCCTCACCGCGCTTTCGCCCATCGACGGCCGCTACGCCAGCAAGACCGAAGCGCTGCGCGACTGGCTCT
CCGAAGCCGCCTTCATGCGCCACCGCGTCACCGTCGAGGTGCACTGGCTGATCGCGCTGTCGCGCGCCGGCTTCGCGGAA
GTCCCGCGCTTCTCCGATGCGTCCGAACAATTCCTGCTGCAGCTCGCCGAGCGCTTCACCGCGCACGACGCCGCCCGCAT
CAAGGACATCGAGCGCGTGACGAACCACGACGTGAAGGCCGTCGAATACTGGCTGAAGGAATCGGTGAAGGGCCAGGAAG
AACTCGAAAAAGCCAGCGAATTCATCCACTTCGCGTGCACGTCGGAAGACATCAACAACACGTCGCACGGGATGATGCTC
GCCGGCGCGCGCGAACACGTGATCCTGCCGGCGCTGCGCACGGTCTACCAGCGCCTCGTCGCACTCGCACACGCGCACGC
CGAGCAGCCGATGCTGTCGCGCACGCACGGCCAGCCGGCCAGCCCGACGACGCTCGGCAAGGAACTCGCGAACGTCGCGG
CCCGCCTCGCCCGTGCGATCACGCGCATCGAGAAGGTCGAGATCCTCGGCAAGATGAACGGCGCGGTCGGCAACTTCAAC
GCGCATCTGTCCGCGTATCCGGAATTCGACTGGGAAGCGTTCTCGCGCGACGTGATCGAGAACCGCCTCAAGCTCACGTT
CAACCCGTACACGATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTTCGACGCCGTGGCGCGCGCGAACACGATCC
TGCTCGACCTCGACCGCGACGTGTGGGGCTACATCTCGGTCGGCTACTTCAAGCAGAAGACGAAGGCCGGTGAAATCGGC
TCGTCGACGATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACTCGGAAGGCAACCTCGGCCTCGCCAACGCGACGCT
GCGCCACCTCGCCGACAAGCTGCCGGTGTCGCGCTGGCAGCGCGACCTGACCGACTCGACGGTGCTGCGCAACATGGGCG
TCGCGCTCGGCTATTCGCTGCTCGCGTACGACTCGCTGATCCGCGGCCTCGACAAGCTCGAAGTGAACCCGCAGCGCCTG
AACGAAGACCTCGACAACTGCTGGGAAGTGCTCGCCGAGCCGGTGCAGACGGTGATGCGCCGCTACGGCATCGAGAACCC
GTACGAGCAGCTGAAGGAGCTGACGCGCGGCAAGGGCATCACGCGCGAAGCGCTGCAGGAGTTCGTCGGCACGCTCGCGA
TCCCGCAAGAGGCGAAGGATCTCCTGCTCGCGATGTCGCCTGCGTCGTACATCGGCAAGGCCGTCGAACTCGCGAAGCGG
ATCGCGTAA

Upstream 100 bases:

>100_bases
GCGGCGAGCGGCCCGGCGCCCCGTCGCGAACGGGCGCCGAGACAGCATCCGCACGCGGAGACGCCCGCCGGCCGCGCGCG
ACCGGGATCGTCCCGCGCCA

Downstream 100 bases:

>100_bases
GCGCCGCCGTCCGAGCACGTAAAAAAGCCCGATGCGAGCAATCGCATCGGGCTTTTTTGTTGCCTGCCGCAACAGCGTCA
GAACGAGATCATCCGCCCCG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MVKCRILLPLARPGPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
VPRFSDASEQFLLQLAERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQEELEKASEFIHFACTSEDINNTSHGMML
AGAREHVILPALRTVYQRLVALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFN
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIG
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRL
NEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQEAKDLLLAMSPASYIGKAVELAKR
IA

Sequences:

>Translated_482_residues
MVKCRILLPLARPGPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
VPRFSDASEQFLLQLAERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQEELEKASEFIHFACTSEDINNTSHGMML
AGAREHVILPALRTVYQRLVALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFN
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIG
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRL
NEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQEAKDLLLAMSPASYIGKAVELAKR
IA
>Mature_482_residues
MVKCRILLPLARPGPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMRHRVTVEVHWLIALSRAGFAE
VPRFSDASEQFLLQLAERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQEELEKASEFIHFACTSEDINNTSHGMML
AGAREHVILPALRTVYQRLVALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFN
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYISVGYFKQKTKAGEIG
SSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQRDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRL
NEDLDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQEAKDLLLAMSPASYIGKAVELAKR
IA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=61.4537444933921, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54081; Mature: 54081

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKCRILLPLARPGPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMR
CEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
HRVTVEVHWLIALSRAGFAEVPRFSDASEQFLLQLAERFTAHDAARIKDIERVTNHDVKA
HHEEEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VEYWLKESVKGQEELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLV
HHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
ALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFN
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRD
CCEECCCCCCHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCC
VWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
RDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQEAKDLLLAMSPASYIGKAVELAKR
HCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
IA
CC
>Mature Secondary Structure
MVKCRILLPLARPGPKARPIMSDTRPDTLFALTALSPIDGRYASKTEALRDWLSEAAFMR
CEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
HRVTVEVHWLIALSRAGFAEVPRFSDASEQFLLQLAERFTAHDAARIKDIERVTNHDVKA
HHEEEEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
VEYWLKESVKGQEELEKASEFIHFACTSEDINNTSHGMMLAGAREHVILPALRTVYQRLV
HHHHHHHHCCCHHHHHHHHHHEEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
ALAHAHAEQPMLSRTHGQPASPTTLGKELANVAARLARAITRIEKVEILGKMNGAVGNFN
HHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AHLSAYPEFDWEAFSRDVIENRLKLTFNPYTIQIEPHDYMAELFDAVARANTILLDLDRD
CCEECCCCCCHHHHHHHHHHCCCEEEECCEEEEECCHHHHHHHHHHHHHCCEEEEEECCC
VWGYISVGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLADKLPVSRWQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
RDLTDSTVLRNMGVALGYSLLAYDSLIRGLDKLEVNPQRLNEDLDNCWEVLAEPVQTVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RYGIENPYEQLKELTRGKGITREALQEFVGTLAIPQEAKDLLLAMSPASYIGKAVELAKR
HCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]