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The map label for this gene is yiaJ [H]

Identifier: 78065175

GI number: 78065175

Start: 587848

End: 588747

Strand: Direct

Name: yiaJ [H]

Synonym: Bcep18194_A3699

Alternate gene names: 78065175

Gene position: 587848-588747 (Clockwise)

Preceding gene: 78065174

Following gene: 78065176

Centisome position: 15.91

GC content: 69.78

Gene sequence:

>900_bases
ATGACCAAGATGCCCGACACACTTGCCCTCGACGCCCGGCGCGCGACGCCGGACGATGCATCGCTGACCGACAGCATCGG
CGCGACCAGTACGCCGCTCGATCTCGCTGCGCAGCAGGCCGGCACGCAGACGTTGCTGCGCGGTCTTGCGATCCTCGAGG
CGATCGCGAACGGCGCGCGCGACATGCGCGCGATCGGCGCCGCGCTCGGCACGACGCGCAGCACCACGCACCGCCTCGTC
AGCAGCCTCGTGCAGGCGCGCTACCTGCGCCAGGTGCAGGGCGGCTACCTGCTCGGCCCGAAGCTGATCGAGCTCGGCAC
GATCGCGCTCGAGCAGATGCCGCTCACCGCGGTGGCGCGCCCGCACCTCGAAGCGCTCGCGGAAGCGACGCTCGACACGA
TCCATCTCGGCGTGCGCGACGGCGACGACGTGCTGTACATCGACAAGATTCCCGGCACGCGCGGCCTCGAGATGCGCTCG
CGCGTCGGCCACCGGATGCCGCTCGCGTCGACCGGCATCGGCAAGGCGATGATGCTCGACACCGATCCCGACACGTGGCG
CTCGCTGTTCGAGGCTGCCCGGCGTGCGCTCGCCGGCGTCAATTTCAAGCCGGACAACCGGCCCGAGACGAGCGCGTTCC
TGCAGCGCATGGCGCACTACGCGGCCGGCGGTTACACGTTCGATCTCGAGGAAAACGAGGCGTCGATCCGCTGCGTGGCC
GCGCCGATCCGCGATGCATCGGGCGCGATCGTGGCGGCCGTGTCGGTCGCGAGCACGATTCCGTACATGCCGCACGACCG
GATGGACGAACTGATTCCGCTCGTGCAGCGCGAAGCGCGAGCCATTTCCGCGGAACTGGGCTGGAGCCCGCCGCAGGGTA
CCCGCAGGATCAAACGATGA

Upstream 100 bases:

>100_bases
CGCTTTATTCGGTGCATCGACCGCCGCCTCGTCGCGACGCCGGACGCAGTTCCGGCATCGCGCGAACGGCTTCACCGGTT
CCGTTGAAGAAGCCCGCCCC

Downstream 100 bases:

>100_bases
CGACTTCGACCCGGCCCGTTCCGGATGCCAGCTCCGCCACCCCGTCGCTGATCGCGCTCGACTGGGGCACGACGTCGCTG
CGCGCTTACCTGTACGACGC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTKMPDTLALDARRATPDDASLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLV
SSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRS
RVGHRMPLASTGIGKAMMLDTDPDTWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR

Sequences:

>Translated_299_residues
MTKMPDTLALDARRATPDDASLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLV
SSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRS
RVGHRMPLASTGIGKAMMLDTDPDTWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
>Mature_298_residues
TKMPDTLALDARRATPDDASLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLVS
SLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSR
VGHRMPLASTGIGKAMMLDTDPDTWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVAA
PIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=258, Percent_Identity=32.1705426356589, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1788131, Length=266, Percent_Identity=31.9548872180451, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=29.2, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI87082359, Length=252, Percent_Identity=28.968253968254, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788581, Length=249, Percent_Identity=30.9236947791165, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786715, Length=253, Percent_Identity=27.6679841897233, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790752, Length=256, Percent_Identity=23.4375, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32216; Mature: 32085

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMPDTLALDARRATPDDASLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGAR
CCCCCCHHEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DMRAIGAALGTTRSTTHRLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHH
PHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLD
HHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEC
TDPDTWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
ECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
TKMPDTLALDARRATPDDASLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGAR
CCCCCHHEECCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DMRAIGAALGTTRSTTHRLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHH
PHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLD
HHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEC
TDPDTWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
ECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]