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The map label for this gene is mpl [H]

Identifier: 78065168

GI number: 78065168

Start: 579270

End: 580619

Strand: Direct

Name: mpl [H]

Synonym: Bcep18194_A3692

Alternate gene names: 78065168

Gene position: 579270-580619 (Clockwise)

Preceding gene: 78065157

Following gene: 78065169

Centisome position: 15.68

GC content: 68.07

Gene sequence:

>1350_bases
ATGGGCGGTCTCGCCGTACTCGCGCGCGAGGCGGGCCACACGGTGACGGGTTGCGACGCGGGCGTCTATCCGCCGATGAG
CACGCAGCTCGAGGCGCAGGGCATCACGCTGATCGAGGGCTACGGCGCCGAGCAGATCGACCTGAAACCGGACCTGTTCG
TGATCGGCAACGTCGTCACGCGCGGCAATCCGCTGATGGAAGCAATCCTGGATCGCGGCCTGCCGTACGTGTCGGGCCCG
CAGTGGCTCGGCGAGCACGTGCTGGCCGGCAAATGGGTGCTCGCGGTCGCGGGCACGCACGGCAAGACCACCACGTCGTC
GATGCTTGCGTGGCTGCTGGAAGACTCGGGCCTGAATCCGGGCTTCCTGATCGGCGGCGTGCCGCTGAACTTCGGCGTGT
CGGCGCGGCTCACCGATTCGAGCTTCTTCGTGATCGAGGCCGACGAATACGATACGGCGTTCTTCGACAAGCGCTCGAAG
TTCGTCCACTATCGGCCGCGCACCGCGGTGCTGAACAATCTCGAATTCGATCACGCCGACATCTTCCCGGATCTCGCCGC
GATCGAGACGCAATTCCACCATCTCGTCCGTACCGTGCCCGGTGTCGGCCGGATCGTCACGAACGGCCGCTCGGACGCGC
TCGAGCGCGTGCTGGCGCGCGGTTGCTGGAGCGAGGTCGAGCGGTTCGGCGTCGACGGCGGCTGGCAGGCGCTGCCGGCC
GAGGACGGTGTGCCGGTCGACGAGCGCTTCGCGGTGTATTCGCACGCGGAACGTGTCGGTGAAGTTACGTGGCAAGTACA
GGGCGATCACAACCGGATGAACGCGCTCGCGGCGATCGCGGCTGCGCGCCACGTCGGTGTGCCGCCCGCGCAGGCGGCCG
AATCCCTCGCGTCGTTCCGCAACGTGAAGCGCCGGATGGAAGTGCGCGGCAGCGTGGACGGCGTGACCGTCTACGACGAC
TTCGCGCACCATCCGACCGCGATCGAAACCACGATCGCCGGCCTTCGTGCACGCATCGGCAGCGAGAATGCTCGCATCCT
CGCCGTGCTCGAGCCGCGTTCGAACACGATGAAGCTCGGCGTGATGAAGTCGCAATTGCCGGCGAGCCTGGCCGATGCCG
ATCTCGTGTTCGGCTACGGCGCGCCGACCGGGCGCGACGCGCTCGGCTGGAACCTGGCCGAGGCGCTGGCGCCGCTGGGC
GAGAAGGCGCACGCATTCGACGATCTGCACCTGCTGGTGAAGTCCGTCGTCGCAGCCGCGCGCCCGGGCGACCACGTGCT
CGTGATGAGCAACGGCGGCTTCGGCGGCGTGCACCAGAAGCTGCTCGATGCGCTCGGGAGCCGCACGTGA

Upstream 100 bases:

>100_bases
CCGCCGCGCCGCACACGCATCCTTTCCGAATCGACGGCGCCCGGCGGCGCGAAAGCATTCATCTATGCACATCCACATTC
TTGGCATCTGCGGCACCTTC

Downstream 100 bases:

>100_bases
TCCTGTACCTGCACGGCTTCCGGTCGTCGCCGGAATCGCAGAAGTCGCGCCAGCTCGCCGCGCGCATGGCCGAGCTCGGC
CGCACCGACGAGTGGCGGTG

Product: UDP-N-acetylmuramate/L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQIDLKPDLFVIGNVVTRGNPLMEAILDRGLPYVSGP
QWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDSGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSK
FVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVERFGVDGGWQALPA
EDGVPVDERFAVYSHAERVGEVTWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFRNVKRRMEVRGSVDGVTVYDD
FAHHPTAIETTIAGLRARIGSENARILAVLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLG
EKAHAFDDLHLLVKSVVAAARPGDHVLVMSNGGFGGVHQKLLDALGSRT

Sequences:

>Translated_449_residues
MGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQIDLKPDLFVIGNVVTRGNPLMEAILDRGLPYVSGP
QWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDSGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSK
FVHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVERFGVDGGWQALPA
EDGVPVDERFAVYSHAERVGEVTWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFRNVKRRMEVRGSVDGVTVYDD
FAHHPTAIETTIAGLRARIGSENARILAVLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLG
EKAHAFDDLHLLVKSVVAAARPGDHVLVMSNGGFGGVHQKLLDALGSRT
>Mature_448_residues
GGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQIDLKPDLFVIGNVVTRGNPLMEAILDRGLPYVSGPQ
WLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDSGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKF
VHYRPRTAVLNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVERFGVDGGWQALPAE
DGVPVDERFAVYSHAERVGEVTWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFRNVKRRMEVRGSVDGVTVYDDF
AHHPTAIETTIAGLRARIGSENARILAVLEPRSNTMKLGVMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLGE
KAHAFDDLHLLVKSVVAAARPGDHVLVMSNGGFGGVHQKLLDALGSRT

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=448, Percent_Identity=59.5982142857143, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1786279, Length=362, Percent_Identity=27.9005524861878, Blast_Score=105, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48105; Mature: 47974

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQIDLKPDLFVIGNVVT
CCCCEEEEHHCCCEEECCCCCCCCCCCCCEECCCEEEEECCCCCEECCCCCEEEEECHHH
RGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDSGLNP
CCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
GFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHAD
CEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHHHHHCCCCCCHHH
IFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVERFGVDGGWQALPA
HCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
EDGVPVDERFAVYSHAERVGEVTWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFR
CCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
NVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRARIGSENARILAVLEPRSNTMKLG
HHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEE
VMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLGEKAHAFDDLHLLVKSVVAAA
EEHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RPGDHVLVMSNGGFGGVHQKLLDALGSRT
CCCCEEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
GGLAVLAREAGHTVTGCDAGVYPPMSTQLEAQGITLIEGYGAEQIDLKPDLFVIGNVVT
CCCEEEEHHCCCEEECCCCCCCCCCCCCEECCCEEEEECCCCCEECCCCCEEEEECHHH
RGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWLLEDSGLNP
CCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCC
GFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHAD
CEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHHHHHCCCCCCHHH
IFPDLAAIETQFHHLVRTVPGVGRIVTNGRSDALERVLARGCWSEVERFGVDGGWQALPA
HCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
EDGVPVDERFAVYSHAERVGEVTWQVQGDHNRMNALAAIAAARHVGVPPAQAAESLASFR
CCCCCCHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
NVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRARIGSENARILAVLEPRSNTMKLG
HHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEE
VMKSQLPASLADADLVFGYGAPTGRDALGWNLAEALAPLGEKAHAFDDLHLLVKSVVAAA
EEHHHCCCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
RPGDHVLVMSNGGFGGVHQKLLDALGSRT
CCCCEEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]