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The map label for this gene is ampD [H]

Identifier: 78065154

GI number: 78065154

Start: 563762

End: 564352

Strand: Direct

Name: ampD [H]

Synonym: Bcep18194_A3678

Alternate gene names: 78065154

Gene position: 563762-564352 (Clockwise)

Preceding gene: 78065153

Following gene: 78065155

Centisome position: 15.26

GC content: 68.36

Gene sequence:

>591_bases
ATGAGCGACGCGCTGCTGCTGTCGGTCGACGCGAACGGCTGGGTGCGCGAAGCGCGCCATGCGCCGTCGCCGAACTTCGA
GGTGCGGCCCGCGGGCGCGGTGCCGACGCTCGTGGTCGTCCACAACATCAGCCTGCCGCCCGGCGAATTCGGTGGCGATG
CAATCGAGGCGCTGTTCCTGAACCGCCTCGATTGCGATGCGCACCCTTATTACCAGAGCCACCTGCGCGACGTGCGCGTG
TCCGCGCACTTCCTGATCCGCCGCAGCGGCGAGCTCGTGCAGTTCGTGTCGTGCGACGAGCGCGCGTGGCACGCGGGCTC
GTCCGAGTTCTTTGGCCGCGCGCGCTGCAACGACTTCTCGATCGGCATCGAGCTCGAAGGCGCGGACGACGTGCCGTTCG
ACGACGCGCAGTATGCGATGCTCGCCGCGCTCTCCCGCACGCTCGCGGCACGCTACCCGGTCGACGCATTCGCCGGGCAC
TCGGACGTCGCGCCGGGCCGCAAGACCGACCCGGGCCCGCATTTCGATTGGCAACGCTTCGCGAGCGATGCCGGTTTTTC
CGCCGAATACTTTCCTTTCCGTCAGCACTGA

Upstream 100 bases:

>100_bases
ATGCGCCGAAGGGCGACGCCGTTGCCGCGGGCGGCGAATACTTCTGCAGTGCCGAGCACGCGCAGCGCCACGCCGCACGG
TCGAGCGGTCGCGACGCACG

Downstream 100 bases:

>100_bases
TCGCGGGGTTTTTTGACCGGAGAAAGTTTTTTCTCCGACCAATGCCTTGTCGTGTCTGCGCGTGAGCGATGTCAAGACCT
CCATCGCAAATTATCCGTTT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSDALLLSVDANGWVREARHAPSPNFEVRPAGAVPTLVVVHNISLPPGEFGGDAIEALFLNRLDCDAHPYYQSHLRDVRV
SAHFLIRRSGELVQFVSCDERAWHAGSSEFFGRARCNDFSIGIELEGADDVPFDDAQYAMLAALSRTLAARYPVDAFAGH
SDVAPGRKTDPGPHFDWQRFASDAGFSAEYFPFRQH

Sequences:

>Translated_196_residues
MSDALLLSVDANGWVREARHAPSPNFEVRPAGAVPTLVVVHNISLPPGEFGGDAIEALFLNRLDCDAHPYYQSHLRDVRV
SAHFLIRRSGELVQFVSCDERAWHAGSSEFFGRARCNDFSIGIELEGADDVPFDDAQYAMLAALSRTLAARYPVDAFAGH
SDVAPGRKTDPGPHFDWQRFASDAGFSAEYFPFRQH
>Mature_195_residues
SDALLLSVDANGWVREARHAPSPNFEVRPAGAVPTLVVVHNISLPPGEFGGDAIEALFLNRLDCDAHPYYQSHLRDVRVS
AHFLIRRSGELVQFVSCDERAWHAGSSEFFGRARCNDFSIGIELEGADDVPFDDAQYAMLAALSRTLAARYPVDAFAGHS
DVAPGRKTDPGPHFDWQRFASDAGFSAEYFPFRQH

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=171, Percent_Identity=58.4795321637427, Blast_Score=204, Evalue=3e-54,
Organism=Escherichia coli, GI1787092, Length=127, Percent_Identity=40.1574803149606, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21662; Mature: 21531

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDALLLSVDANGWVREARHAPSPNFEVRPAGAVPTLVVVHNISLPPGEFGGDAIEALFL
CCCCEEEEECCCCHHHHHHCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHH
NRLDCDAHPYYQSHLRDVRVSAHFLIRRSGELVQFVSCDERAWHAGSSEFFGRARCNDFS
HHCCCCCCCHHHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHCCCCHHHCCCCCCCCEE
IGIELEGADDVPFDDAQYAMLAALSRTLAARYPVDAFAGHSDVAPGRKTDPGPHFDWQRF
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHH
ASDAGFSAEYFPFRQH
HCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDALLLSVDANGWVREARHAPSPNFEVRPAGAVPTLVVVHNISLPPGEFGGDAIEALFL
CCCEEEEECCCCHHHHHHCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHH
NRLDCDAHPYYQSHLRDVRVSAHFLIRRSGELVQFVSCDERAWHAGSSEFFGRARCNDFS
HHCCCCCCCHHHHHHHHHHHHHEEEEECCCCEEEEECCCCHHHCCCCHHHCCCCCCCCEE
IGIELEGADDVPFDDAQYAMLAALSRTLAARYPVDAFAGHSDVAPGRKTDPGPHFDWQRF
EEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCHHHH
ASDAGFSAEYFPFRQH
HCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]