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The map label for this gene is proB [H]

Identifier: 78065145

GI number: 78065145

Start: 555275

End: 556393

Strand: Direct

Name: proB [H]

Synonym: Bcep18194_A3669

Alternate gene names: 78065145

Gene position: 555275-556393 (Clockwise)

Preceding gene: 78065144

Following gene: 78065148

Centisome position: 15.03

GC content: 66.22

Gene sequence:

>1119_bases
ATGCGTTCGATCATCGCGGATTCGAAGCGATTGGTGGTGAAGGTGGGCTCCAGCCTCGTGACCAACGACGGCAAGGGGCT
CGATCATGCAGCAATCGGCCGTTGGGCGGCTCAGATCGCCGCACTGCGCGCCCAGGGCAAGGAAGTCGTGCTCGTCAGTT
CGGGCGCGATTGCCGAAGGGATGCAGCGGCTAGGCTGGAGCAAGCGGCCGCGGGAAATCGACGAGTTGCAGGCTGCCGCC
GCAGTCGGCCAGATGGGGCTCGCGCAGGTCTATGAGAGCCGCTTCACCGAGCACGACATCCGCACCGCGCAGATCCTGCT
GACGCATGCCGACCTGGCGGACCGCGAGCGTTACCTGAATGCACGTTCGACGATGCTGACGCTGTTGCGGCTCGGCGTCG
TGCCGATCATCAACGAGAACGACACGGTCGTCACCGACGAAATCAAGTTCGGCGACAACGACACGCTCGGCGCGCTCGTT
GCAAACCTGATCGAAGGCGATGCACTGATCATCCTGACCGACCAGTCGGGGCTGTTCACCGCCGATCCGCGCAAGGATCC
GAATGCGACGCTCGTTGCAGAAGCCAATGCAGGCGCGCCGGAACTCGAGGCGATGGCCGGCGGCGCGGGTTCGAGCCTCG
GCCGCGGCGGGATGCTGACGAAGATCCTCGCGGCGAAGCGCGCGGCACACAGCGGCGCGAACACCGTGATCGCGAGCGGC
CGGGAGACCGACGTGCTCGTGCGTCTGGCGGCCGGCGAGGCGATCGGCACGCAACTGATCGCACGTACCGCGCGGATGGC
GGCGCGCAAGCAGTGGATGGCCGACCACCTTCAGGTGCGCGGCCATGTCGTGATCGACGCGGGCGCGGTCGAGAAGCTGA
CGGCCGGCGGCAAGAGCCTGCTGCCGATCGGCGTGATCGACGTGCAGGGTGCGTTCGCGCGCGGTGAAGTCATCGCGTGT
GTTGGCCCCGACGGGCGCGAAGTGGCGCGTGGGCTCACGAACTACAGCAGTGCGGAGACGAAACTGATTCACCGCAAACC
GAGCGGCGAGATCGAATCCGTGCTCGGCTACATGCTGGAGCCCGAGTTGATCCATCGCGACAACCTGGTGCTGGTGTGA

Upstream 100 bases:

>100_bases
GACGCCTGAGCGATTCCGGAACGCGCCGGGCGTGCCGCCCGGCGTCAGCGTTCAATCGCGCCCCGGCCGGGCAGGCAGCA
GATACAGGAGACAGTGCAGG

Downstream 100 bases:

>100_bases
CCATACGTGCCGGTTCCGGCCGTCAAATGAAAAAGCCCGCGTGATGCGGGCTTTTTTCATGCGGCGGACAGGGGCGCGGC
GGGTGCCGCGCGCCGTCAGC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLNARSTMLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLEPELIHRDNLVLV

Sequences:

>Translated_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLNARSTMLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLEPELIHRDNLVLV
>Mature_372_residues
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEGMQRLGWSKRPREIDELQAAA
AVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLNARSTMLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALV
ANLIEGDALIILTDQSGLFTADPRKDPNATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSLLPIGVIDVQGAFARGEVIAC
VGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLEPELIHRDNLVLV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=362, Percent_Identity=33.1491712707182, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI62912457, Length=360, Percent_Identity=33.3333333333333, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.896174863388, Blast_Score=277, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI17569967, Length=281, Percent_Identity=35.2313167259786, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17569969, Length=281, Percent_Identity=35.2313167259786, Blast_Score=141, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6320506, Length=420, Percent_Identity=37.1428571428571, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321822, Length=414, Percent_Identity=32.3671497584541, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI21357643, Length=350, Percent_Identity=30.8571428571429, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39319; Mature: 39319

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHH
ARSTMLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCHHHHHCCCCCC
LPIGVIDVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLE
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC
>Mature Secondary Structure
MRSIIADSKRLVVKVGSSLVTNDGKGLDHAAIGRWAAQIAALRAQGKEVVLVSSGAIAEG
CCCCCCCCCEEEEEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHH
MQRLGWSKRPREIDELQAAAAVGQMGLAQVYESRFTEHDIRTAQILLTHADLADRERYLN
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHH
ARSTMLTLLRLGVVPIINENDTVVTDEIKFGDNDTLGALVANLIEGDALIILTDQSGLFT
HHHHHHHHHHHCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDPNATLVAEANAGAPELEAMAGGAGSSLGRGGMLTKILAAKRAAHSGANTVIASG
CCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
RETDVLVRLAAGEAIGTQLIARTARMAARKQWMADHLQVRGHVVIDAGAVEKLTAGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCEEEEECCCHHHHHCCCCCC
LPIGVIDVQGAFARGEVIACVGPDGREVARGLTNYSSAETKLIHRKPSGEIESVLGYMLE
CEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHCC
PELIHRDNLVLV
CHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA