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The map label for this gene is pilF [H]

Identifier: 78065139

GI number: 78065139

Start: 549021

End: 550298

Strand: Reverse

Name: pilF [H]

Synonym: Bcep18194_A3663

Alternate gene names: 78065139

Gene position: 550298-549021 (Counterclockwise)

Preceding gene: 78065140

Following gene: 78065138

Centisome position: 14.9

GC content: 70.5

Gene sequence:

>1278_bases
ATGCATTTCCCTCCTCCCGGGGCGCCGCTGCGCACGCAGCAGCCGCCCGCTCACTCCGATGTGTCGCCCGCCACCGCATC
GCGCGCGCTCGACGCGGCCCAGCTCGACGATGCGCCAGCCGTGCGGCTGCTGACGGACACGCTTCACGCGGCACGCGTGC
GCGACGCATCCGACATCCACGTCGAACCATTCGAAGCCGGCTGGCGCATCCGCCTGCGCATCGACGGCGTGCTGCATGAA
CATGCGCGGCCGCCCCTGCATCTGCGCGACGCGCTCGTCACACGAATCAAGGTGCTCGCGCGCATGGACATCGCCGAGCG
GCGGCTCCCGCAGGACGGCAGGCTGCGCATCGCGATCGACGGCGGTACGCGCGGCGACTACCGGGTCAGCTCGCTGCCCA
CGTTGTTCGGTGAAAAGCTGGTGCTGCGACGGCTCGAAACCATGCCACCCGACCTCACACTCGCACGCCTCGGCTTCGAC
GCGCAGCAGGCAGCCGCGATGGAGGCCGCGATACGCGCACCGCATGGCCTCGTGCTCGTCACGGGCCCCACCGGCAGCGG
CAAGACGCTGTCGCTCTACTGCGCGCTGCAGATGCTCGATCGCGACGCGCACAACGTCTGCACGGTCGAGGATCCCGCCG
AGATTCAGCTCGACGGCATCAGCCAGGTCGGTGTCGCCGAAAAAGCGGGGCTCACGTTCGCGACGGCACTGCGCGCGCTA
CTGCGGCAGGATCCGGACATCATCATGGTCGGCGAGATTCGCGATGCGGAGACAGCCGACGTCGCGATCAAGGCAGCGCA
GACCGGCCATCTCGTGCTGTCGACGCTGCACACGAACGACGCGCCGGCCGCCGTCGCGCGGCTGCTCGACATCGGCGTCG
CACCGTACAACCTCGCGGCCGCACTGCACCTCGTCACCGCGCAACGCCTCGTCCGGCGGCTGTGCACCGCCTGCCGCATA
CACTCGGACGCACCGGTGCCTGTCCTGCGCGAAACCGGCTGCGACCCGGCCGCCCTCGCAGCCGGATGGCGGCCGTTCGT
CGCGCGAGGCTGTCCGGCCTGCCACGGCATCGGCTATCGCGGCCGTGTCGGCCTGCATCAGACCATGCCGGTATCGCCCG
GGCTGGCAGAACGCATCGTCGCACGGGCGAGTGTCGGCGAACTCGCGCAATGCGTGGCGGCCGAAGGCGTGCGCAGTCTG
CGCGACGCGGCGCTCGCGCGCGTTCACGACGGCACGACCAGCATCGCGGAAGCCCTCGCCGCGACACCTGCCACATGA

Upstream 100 bases:

>100_bases
GCGTCACCTCCCGCTAGCCGAACGGCTGACTGCCAGCGCCGCCCGCGACGCGCGACCATCTGCTGGTCACGGTCAACAGG
CGGCCAGCGTGCCGGCTCAC

Downstream 100 bases:

>100_bases
GCATGCGCACGCCGCCGCGCGAAACCCGCTTCGCGTGGCGCGGCCGCCGTCGCGACGGCACGTCGCGCCGAGGGAACGTC
ATCGCATTCGACGCCGCGAG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MHFPPPGAPLRTQQPPAHSDVSPATASRALDAAQLDDAPAVRLLTDTLHAARVRDASDIHVEPFEAGWRIRLRIDGVLHE
HARPPLHLRDALVTRIKVLARMDIAERRLPQDGRLRIAIDGGTRGDYRVSSLPTLFGEKLVLRRLETMPPDLTLARLGFD
AQQAAAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVEDPAEIQLDGISQVGVAEKAGLTFATALRAL
LRQDPDIIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALHLVTAQRLVRRLCTACRI
HSDAPVPVLRETGCDPAALAAGWRPFVARGCPACHGIGYRGRVGLHQTMPVSPGLAERIVARASVGELAQCVAAEGVRSL
RDAALARVHDGTTSIAEALAATPAT

Sequences:

>Translated_425_residues
MHFPPPGAPLRTQQPPAHSDVSPATASRALDAAQLDDAPAVRLLTDTLHAARVRDASDIHVEPFEAGWRIRLRIDGVLHE
HARPPLHLRDALVTRIKVLARMDIAERRLPQDGRLRIAIDGGTRGDYRVSSLPTLFGEKLVLRRLETMPPDLTLARLGFD
AQQAAAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVEDPAEIQLDGISQVGVAEKAGLTFATALRAL
LRQDPDIIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALHLVTAQRLVRRLCTACRI
HSDAPVPVLRETGCDPAALAAGWRPFVARGCPACHGIGYRGRVGLHQTMPVSPGLAERIVARASVGELAQCVAAEGVRSL
RDAALARVHDGTTSIAEALAATPAT
>Mature_425_residues
MHFPPPGAPLRTQQPPAHSDVSPATASRALDAAQLDDAPAVRLLTDTLHAARVRDASDIHVEPFEAGWRIRLRIDGVLHE
HARPPLHLRDALVTRIKVLARMDIAERRLPQDGRLRIAIDGGTRGDYRVSSLPTLFGEKLVLRRLETMPPDLTLARLGFD
AQQAAAMEAAIRAPHGLVLVTGPTGSGKTLSLYCALQMLDRDAHNVCTVEDPAEIQLDGISQVGVAEKAGLTFATALRAL
LRQDPDIIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAAALHLVTAQRLVRRLCTACRI
HSDAPVPVLRETGCDPAALAAGWRPFVARGCPACHGIGYRGRVGLHQTMPVSPGLAERIVARASVGELAQCVAAEGVRSL
RDAALARVHDGTTSIAEALAATPAT

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=388, Percent_Identity=44.8453608247423, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI1786296, Length=388, Percent_Identity=45.8762886597938, Blast_Score=308, Evalue=4e-85,
Organism=Escherichia coli, GI87082188, Length=268, Percent_Identity=35.0746268656716, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 45211; Mature: 45211

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS51007 CYTC ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFPPPGAPLRTQQPPAHSDVSPATASRALDAAQLDDAPAVRLLTDTLHAARVRDASDIH
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCE
VEPFEAGWRIRLRIDGVLHEHARPPLHLRDALVTRIKVLARMDIAERRLPQDGRLRIAID
EEECCCCCEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GGTRGDYRVSSLPTLFGEKLVLRRLETMPPDLTLARLGFDAQQAAAMEAAIRAPHGLVLV
CCCCCCEEHHHCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEE
TGPTGSGKTLSLYCALQMLDRDAHNVCTVEDPAEIQLDGISQVGVAEKAGLTFATALRAL
ECCCCCCCEEEHHHHHHHHCCCCCCEEECCCCCCEEECCCCCCCCHHHCCCHHHHHHHHH
LRQDPDIIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAA
HHCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHH
ALHLVTAQRLVRRLCTACRIHSDAPVPVLRETGCDPAALAAGWRPFVARGCPACHGIGYR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHCCCCHHHHCCCCHHHCCCCC
GRVGLHQTMPVSPGLAERIVARASVGELAQCVAAEGVRSLRDAALARVHDGTTSIAEALA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ATPAT
CCCCC
>Mature Secondary Structure
MHFPPPGAPLRTQQPPAHSDVSPATASRALDAAQLDDAPAVRLLTDTLHAARVRDASDIH
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCE
VEPFEAGWRIRLRIDGVLHEHARPPLHLRDALVTRIKVLARMDIAERRLPQDGRLRIAID
EEECCCCCEEEEEECCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
GGTRGDYRVSSLPTLFGEKLVLRRLETMPPDLTLARLGFDAQQAAAMEAAIRAPHGLVLV
CCCCCCEEHHHCHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEE
TGPTGSGKTLSLYCALQMLDRDAHNVCTVEDPAEIQLDGISQVGVAEKAGLTFATALRAL
ECCCCCCCEEEHHHHHHHHCCCCCCEEECCCCCCEEECCCCCCCCHHHCCCHHHHHHHHH
LRQDPDIIMVGEIRDAETADVAIKAAQTGHLVLSTLHTNDAPAAVARLLDIGVAPYNLAA
HHCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHH
ALHLVTAQRLVRRLCTACRIHSDAPVPVLRETGCDPAALAAGWRPFVARGCPACHGIGYR
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHHCCCCHHHHCCCCHHHCCCCC
GRVGLHQTMPVSPGLAERIVARASVGELAQCVAAEGVRSLRDAALARVHDGTTSIAEALA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ATPAT
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]