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The map label for this gene is mutT [H]

Identifier: 78065133

GI number: 78065133

Start: 544651

End: 545094

Strand: Direct

Name: mutT [H]

Synonym: Bcep18194_A3657

Alternate gene names: 78065133

Gene position: 544651-545094 (Clockwise)

Preceding gene: 78065132

Following gene: 78065142

Centisome position: 14.74

GC content: 66.89

Gene sequence:

>444_bases
ATGAGCGCGGATCTCGCACAAGGCGCGGTGCGCGCACCCGATGGCCGCAAGGTGACGGAAGTCGCGGTCGGCGTGATGGT
GCAGCCGGACGGTAGCTACCTGCTCGCGCAGCGGCTGCAAGGCAAGCCGTATGAAGGCTACTGGGAATTTCCGGGCGGCA
AGCTGGAGGCCGGCGAAAGCGTCGAGGACGCGCTTGCGCGCGAACTGCACGAGGAGCTCGGTATCGAAGTCACGGCCAGC
CATCGCTGGCACACGCTCGAGCACGATTATCCGCATGCGTACGTGCGGCTGTACTTCTGCAAGGTGACGGGCTGGACGGG
CGAGCCGCACAGCAAGGAAGGGCAGGCGTTCGTGTGGCAACAACTGCCGGTTGCGGTCGCGCCGCTGCTGCCGGCCGCGC
TGCCGGTGCTCGAACTGCTCGAGAAGGAAGCCGCGTCGGACTGA

Upstream 100 bases:

>100_bases
CACGCGGCGACGCGCTTGTATGGGCGCTCGAGCGCGGCTCGCGGTCGGGGCGCGTCGCGTGGCAGTTTGCGCGTGACTGG
TCGGGCCGCAAGGAGCAGGC

Downstream 100 bases:

>100_bases
CGTCAAGATGGCCCACCGGAAAGGCGGGCCATATCACCAGCGGGCGGCGCCGCGCGCCGCTCAGCTGTCTCGACGGCCTT
CCGTGCCGTCTTCTTCCGAC

Product: NUDIX hydrolase

Products: 8-Oxo-Dgmp; Pyrophosphate. [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MSADLAQGAVRAPDGRKVTEVAVGVMVQPDGSYLLAQRLQGKPYEGYWEFPGGKLEAGESVEDALARELHEELGIEVTAS
HRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQQLPVAVAPLLPAALPVLELLEKEAASD

Sequences:

>Translated_147_residues
MSADLAQGAVRAPDGRKVTEVAVGVMVQPDGSYLLAQRLQGKPYEGYWEFPGGKLEAGESVEDALARELHEELGIEVTAS
HRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQQLPVAVAPLLPAALPVLELLEKEAASD
>Mature_146_residues
SADLAQGAVRAPDGRKVTEVAVGVMVQPDGSYLLAQRLQGKPYEGYWEFPGGKLEAGESVEDALARELHEELGIEVTASH
RWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQQLPVAVAPLLPAALPVLELLEKEAASD

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786288, Length=127, Percent_Identity=34.6456692913386, Blast_Score=76, Evalue=7e-16,
Organism=Escherichia coli, GI1788056, Length=123, Percent_Identity=39.8373983739837, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16176; Mature: 16045

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADLAQGAVRAPDGRKVTEVAVGVMVQPDGSYLLAQRLQGKPYEGYWEFPGGKLEAGES
CCCCCCCCCEECCCCCEEEEEEEEEEECCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCC
VEDALARELHEELGIEVTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQ
HHHHHHHHHHHHHCCEEECCCCEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEH
QLPVAVAPLLPAALPVLELLEKEAASD
HCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SADLAQGAVRAPDGRKVTEVAVGVMVQPDGSYLLAQRLQGKPYEGYWEFPGGKLEAGES
CCCCCCCCEECCCCCEEEEEEEEEEECCCCCCHHHHHHCCCCCCCEEECCCCCCCCCCC
VEDALARELHEELGIEVTASHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSKEGQAFVWQ
HHHHHHHHHHHHHCCEEECCCCEEECCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEH
QLPVAVAPLLPAALPVLELLEKEAASD
HCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Oxo-Dgtp; H2O [C]

Specific reaction: 8-Oxo-Dgtp + H2O = 8-Oxo-Dgmp + Pyrophosphate. [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]