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The map label for this gene is yoaE [H]

Identifier: 78065108

GI number: 78065108

Start: 513006

End: 515081

Strand: Direct

Name: yoaE [H]

Synonym: Bcep18194_A3632

Alternate gene names: 78065108

Gene position: 513006-515081 (Clockwise)

Preceding gene: 78065107

Following gene: 78065109

Centisome position: 13.89

GC content: 63.68

Gene sequence:

>2076_bases
ATGAACGCCGCCATTCAGGTAGCCCGGGCCGTTTGCCCGCATGATTGTCCGGATACGTGCGCAATGCGTGTAACCGTCGA
AAACGGCAAGGCGATCAAGGTCACGGGCGATCCCGACCATCCGCCGACGCAAGGCGTGTTGTGCACGAAAGTCAGTCGAT
ACGCCGATCGCGTTCATCACCCCGACCGCCTCACCGTCCCGCTCAAGCGCATTGGTGCGAAGGGGGAAGGGCGTTTCGTG
CCGATCAGCTGGGACGAGGCATTCGATGAGATCGGCCGTCGTCTCGGTGAAATTGCGGCGCGCGCGCCCGAAGCAATCAT
GCCGTACAGCTATGCGGGGACGATGGGGCTCGTGCAAGGGGAGGGCATCGCCCAGCGGTTCTTCCACAAGCTCGGTGCCT
CGCGGCTCGAGCGCACGATCTGCGCGGCAGCCGGTGCAGCGGGGTTGCGTTACACCTATGGCGGCAGTCTCGGCATGCAC
CTCGAGCATTTCGAGGAAAGCGAGCTGATTCTGATTTGGGGGGCAAACCCGATCGCGTCGAGCCTGCACTTCTGGACGCG
CGCGCAGGAGGCGAAGCGCCGAGGTGCTCGCCTGGTCGCCATCGATCCGTACCGTTCGCTGACGGCGGAGAAGTGCCATC
AGCATATCGCGTTGAAACCCGGCACCGACGGCGCGTTCGCACTCGGCATGATGCACGTGCTGATTACCGAAAACCTGCTC
GATCACGATTACATCGCCAGCCATACGCTCGGTTTCGACGCGCTGAAGGAGCGGGCGCTGTCCTATCCGCCGGAACGCGT
TGCGCAGATCTGCGGGATCGACGCGTCGGAGCTGGTCGACCTTGCGCGTCGCTACGGCGCTACCCGCAAGGCATCGATCC
GCCTCAACTACGGCATGCAGCGCGTCCGCGGCGGTGGCAACGCCGTACGCGCGATCGCCAGTCTGCCGGCGCTGACGGGT
GCCTGGCGGGATCGGGCCGGCGGGCTGTTGCTGTCATCGTCGGAGTCCGCGCCGGTCAACCAGGCTGCGTTGCTACGCCC
GGACTTGATGCCGGGATGGCCGCACAAGCTGCCGCGCATCATCAACATGAACGCGATCGGCGACGCGCTGTTGCACCCGG
GCGATGCAACCTTCGGGCCGAAAGTTGAGGCGGTGATCGTGTACAACTCGAATCCGGTGGCCGTCGCACCGGACTCTTCG
AAGGTCGCGGCAGGGTTCGCGCGTGACGATCTGTTCACGGTCGTTCTCGAGCATTTCAAGACAGATACCGTCGATTTCGC
CGACATTGTGTTGCCGGCAACTACGCAGCTCGAGCATCTGGACATCCACAAATCGTACGGTCACACCTACGTGATGGCGA
ACCTGCCGTCGATTCCACCGGTGGGAGACGCGCGGCCGAATACGGAGATCTTCCGTGGTATCGCGCGCAGTATGGGGCTC
GACGAACCCGCGCTTTATCACAGCGACGAAGAGGTCGCGCGCGCGGCGCTCCGCTGGGACGATCCAGTGCTCGACAGCGA
CTGGGGCACGCTTAAGCGCGATGGCTGGCTGAAGCTCAAGCTGCCGGAGGCGCCGTTCGCGAACGGCGGTTTCCGTACGC
CGTCCGGCAAGTGCGAGTTCTACAGCCCGCGACTCGAGCAGATGGGCGTGGATCCCGTGCCCGACTACCTGCCGCCGTTC
GAATCGGCCGAGGCTGCGCCCGAGCTCGCCGCACGCTATCCGCTCGCGATGATTTCACCGCCGGCGCGTCACTTCCTGAA
CAGTACGTTCGTGAACGTCGACAGCCTGCGTCATACCGAAGGTGAACCGCATCTCGACATCCACCCGTCTGACGCCGATG
AGCGCGGCATCACCGATGGCGACCTCGTACGCATCTTCAACGATCGTGGATCGATGCAGGCGCTCGCGAGAATTACCGAT
CGCGCACGCGCAGGGCTCGTCGTCGGTCTGTCGATCTGGTGGAAGAAGCTGTCACCGGACGGCAGAAACGCGAACGAAGT
GACGAGCCAGGCGCTCACCGATCTTGGCAATTCGGCGACGTTCTACGATTGCCTCGTGGAAGTTGAACGCATTTGA

Upstream 100 bases:

>100_bases
CATCGCTTTCGGCCAAATACCTCAGAATCCGCACGAAATGAACGCTTTATTCGGCGCGTCCACGTAAAATTTGCGAATCC
CCATCTCTGCATTCGAAACG

Downstream 100 bases:

>100_bases
TCGGATATCGCCGTATGATCAGACGAATTTCGACGGAAGTTCGAAGTGGACATCTAACCCTGTTGTCTCAAGGCGAGCGA
GCCGTTACGATGCGTTTTAG

Product: molybdopterin oxidoreductase

Products: Reduced form of N-Oxide compounds [C]

Alternate protein names: NA

Number of amino acids: Translated: 691; Mature: 691

Protein sequence:

>691_residues
MNAAIQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHHPDRLTVPLKRIGAKGEGRFV
PISWDEAFDEIGRRLGEIAARAPEAIMPYSYAGTMGLVQGEGIAQRFFHKLGASRLERTICAAAGAAGLRYTYGGSLGMH
LEHFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIASHTLGFDALKERALSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRIINMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSS
KVAAGFARDDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDIHKSYGHTYVMANLPSIPPVGDARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPVLDSDWGTLKRDGWLKLKLPEAPFANGGFRTPSGKCEFYSPRLEQMGVDPVPDYLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRHTEGEPHLDIHPSDADERGITDGDLVRIFNDRGSMQALARITD
RARAGLVVGLSIWWKKLSPDGRNANEVTSQALTDLGNSATFYDCLVEVERI

Sequences:

>Translated_691_residues
MNAAIQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHHPDRLTVPLKRIGAKGEGRFV
PISWDEAFDEIGRRLGEIAARAPEAIMPYSYAGTMGLVQGEGIAQRFFHKLGASRLERTICAAAGAAGLRYTYGGSLGMH
LEHFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIASHTLGFDALKERALSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRIINMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSS
KVAAGFARDDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDIHKSYGHTYVMANLPSIPPVGDARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPVLDSDWGTLKRDGWLKLKLPEAPFANGGFRTPSGKCEFYSPRLEQMGVDPVPDYLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRHTEGEPHLDIHPSDADERGITDGDLVRIFNDRGSMQALARITD
RARAGLVVGLSIWWKKLSPDGRNANEVTSQALTDLGNSATFYDCLVEVERI
>Mature_691_residues
MNAAIQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHHPDRLTVPLKRIGAKGEGRFV
PISWDEAFDEIGRRLGEIAARAPEAIMPYSYAGTMGLVQGEGIAQRFFHKLGASRLERTICAAAGAAGLRYTYGGSLGMH
LEHFEESELILIWGANPIASSLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
DHDYIASHTLGFDALKERALSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQRVRGGGNAVRAIASLPALTG
AWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRIINMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSS
KVAAGFARDDLFTVVLEHFKTDTVDFADIVLPATTQLEHLDIHKSYGHTYVMANLPSIPPVGDARPNTEIFRGIARSMGL
DEPALYHSDEEVARAALRWDDPVLDSDWGTLKRDGWLKLKLPEAPFANGGFRTPSGKCEFYSPRLEQMGVDPVPDYLPPF
ESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRHTEGEPHLDIHPSDADERGITDGDLVRIFNDRGSMQALARITD
RARAGLVVGLSIWWKKLSPDGRNANEVTSQALTDLGNSATFYDCLVEVERI

Specific function: Terminal Reductase During Anaerobic Growth On Various Sulfoxide And N-Oxide Compounds. Allows E.Coli To Grow Anaerobically On Me(2)So As Respiratory Oxidant. [C]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm Face Of The Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081797, Length=765, Percent_Identity=28.3660130718954, Blast_Score=229, Evalue=5e-61,
Organism=Escherichia coli, GI1787870, Length=784, Percent_Identity=27.5510204081633, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI171474008, Length=761, Percent_Identity=27.2010512483574, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI3868721, Length=729, Percent_Identity=25.9259259259259, Blast_Score=193, Evalue=3e-50,
Organism=Escherichia coli, GI1787231, Length=733, Percent_Identity=25.1023192360164, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87081994, Length=755, Percent_Identity=25.0331125827815, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI145693196, Length=725, Percent_Identity=27.0344827586207, Blast_Score=155, Evalue=1e-38,
Organism=Escherichia coli, GI1788534, Length=360, Percent_Identity=22.2222222222222, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI3868720, Length=263, Percent_Identity=27.7566539923954, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787778, Length=289, Percent_Identity=25.9515570934256, Blast_Score=84, Evalue=4e-17,
Organism=Escherichia coli, GI3868719, Length=368, Percent_Identity=22.8260869565217, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.8.99.- [C]

Molecular weight: Translated: 75459; Mature: 75459

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00932 MOLYBDOPTERIN_PROK_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAIQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHH
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PDRLTVPLKRIGAKGEGRFVPISWDEAFDEIGRRLGEIAARAPEAIMPYSYAGTMGLVQG
CCCEEECHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEEC
EGIAQRFFHKLGASRLERTICAAAGAAGLRYTYGGSLGMHLEHFEESELILIWGANPIAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DHDYIASHTLGFDALKERALSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQ
CCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIASLPALTGAWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCE
INMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSSKVAAGFARDDLFTVVLEHFK
EECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHCCHHHHHHHHHHHHHHC
TDTVDFADIVLPATTQLEHLDIHKSYGHTYVMANLPSIPPVGDARPNTEIFRGIARSMGL
CCCCCHHHEEECCCCCHHHEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYHSDEEVARAALRWDDPVLDSDWGTLKRDGWLKLKLPEAPFANGGFRTPSGKCEF
CCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
YSPRLEQMGVDPVPDYLPPFESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRHTE
CCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEECCHHHHHHHHHHEEHHHHHCCC
GEPHLDIHPSDADERGITDGDLVRIFNDRGSMQALARITDRARAGLVVGLSIWWKKLSPD
CCCCEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCC
GRNANEVTSQALTDLGNSATFYDCLVEVERI
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNAAIQVARAVCPHDCPDTCAMRVTVENGKAIKVTGDPDHPPTQGVLCTKVSRYADRVHH
CCHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCC
PDRLTVPLKRIGAKGEGRFVPISWDEAFDEIGRRLGEIAARAPEAIMPYSYAGTMGLVQG
CCCEEECHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEEC
EGIAQRFFHKLGASRLERTICAAAGAAGLRYTYGGSLGMHLEHFEESELILIWGANPIAS
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHCCCCCCEEEEECCCHHHH
SLHFWTRAQEAKRRGARLVAIDPYRSLTAEKCHQHIALKPGTDGAFALGMMHVLITENLL
HHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHH
DHDYIASHTLGFDALKERALSYPPERVAQICGIDASELVDLARRYGATRKASIRLNYGMQ
CCHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEECCCHH
RVRGGGNAVRAIASLPALTGAWRDRAGGLLLSSSESAPVNQAALLRPDLMPGWPHKLPRI
HHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHCCCCCCCCCCCCCCCE
INMNAIGDALLHPGDATFGPKVEAVIVYNSNPVAVAPDSSKVAAGFARDDLFTVVLEHFK
EECCHHHHHHCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHCCHHHHHHHHHHHHHHC
TDTVDFADIVLPATTQLEHLDIHKSYGHTYVMANLPSIPPVGDARPNTEIFRGIARSMGL
CCCCCHHHEEECCCCCHHHEEEECCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCC
DEPALYHSDEEVARAALRWDDPVLDSDWGTLKRDGWLKLKLPEAPFANGGFRTPSGKCEF
CCCHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCEE
YSPRLEQMGVDPVPDYLPPFESAEAAPELAARYPLAMISPPARHFLNSTFVNVDSLRHTE
CCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEECCHHHHHHHHHHEEHHHHHCCC
GEPHLDIHPSDADERGITDGDLVRIFNDRGSMQALARITDRARAGLVVGLSIWWKKLSPD
CCCCEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEHHHHHHHCCCC
GRNANEVTSQALTDLGNSATFYDCLVEVERI
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: May Bind 4Fe-4S Cluster. [C]

Metal ions: Fe; Mo [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-Oxide; Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

Specific reaction: Reduces Various N-Oxide And Sulfoxide Compounds Including Trimethylamine N-Oxide. [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]