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The map label for this gene is trpE [H]

Identifier: 78065097

GI number: 78065097

Start: 494821

End: 496314

Strand: Reverse

Name: trpE [H]

Synonym: Bcep18194_A3621

Alternate gene names: 78065097

Gene position: 496314-494821 (Counterclockwise)

Preceding gene: 78065098

Following gene: 78065096

Centisome position: 13.44

GC content: 65.26

Gene sequence:

>1494_bases
ATGACCGAACTCGAATTTCAATCGCTCGCGAACGAAGGCTACAACCGCATCCCGCTGATCGCGGAAGCCCTCGCCGATCT
CGAAACGCCGCTGTCCCTCTACCTGAAGCTGGCCCAGCCCGAACGCTCGGGCGCCAACTCGTTCCTGCTCGAATCGGTGG
TCGGCGGCGAACGCTTCGGCCGCTATTCGTTCATCGGCCTGCCCGCCCGTACGCTCGTGCGTACCCGCAACGGCGTGTCG
GAAGTCGTGCGCGACGGCCAGGTCGTCGAGACGCATGACGGTGACCCGTTCCAGTTCATCGAATCGTTCCAGGCGCGCTT
CAAGGTGGCGCAGCGCCCGGGCCTGCCGCGCTTCTGCGGCGGTCTCGCCGGCTATTTCGGCTACGACGCGGTGCGTTACA
TCGAGAAGAAGCTCGCGAACACCACGCCGCGCGACGATCTCGGCCTGCCCGATATCCAGTTGCTGCTGACCGAGGAAGTG
GCGGTCATCGACAACCTCGCCGGCAAGCTGTACCTGATCATCTACGCCGACCCGAGCCAGGCCGAAGCCTATACGAAGGC
GAAGCAGCGCCTGCGCGAACTGAAGCAGCGCCTGCGCACGACCGTGCAGCCGCCGGTCACGTCGGCGAGCGTGCGCACCG
AGACGTTCCGCGAGTTCAAGAAGGACGACTATCTGGCCGCCGTGCGCCAGGCGAAAGAATACATCGCGGCCGGCGAGCTG
ATGCAGATCCAGGTCGGCCAGCGCCTGACGAAGCCGTATCGCGACAATCCGCTGTCGCTGTATCGCGCACTGCGTTCGCT
GAACCCGTCGCCGTACATGTACTACTACAACTTCGGCGATTTCCACGTGGTCGGCGCATCGCCGGAAATCCTCGTGCGCC
AGGAAAAGCGTGGCGAAGACCAGATCGTCACGATCCGCCCGCTGGCCGGCACGCGCCCGCGCGGCAACACGCCCGAGCGC
GACGCGGAACTCGCGACCGAGCTGCTCAACGATCCGAAGGAGATCGCCGAGCACGTGATGCTGATCGACCTCGCGCGCAA
CGACGTCGGCCGCATCGCGGAAATCGGCTCGGTGCACGTGACCGACCAGATGGTCATCGAGAAATACTCGCACGTGCAGC
ACATCGTCAGCTCGGTCGAAGGCAAGCTGAAGCCCGGCATGACGAACTACGACGTGCTGCGCGCGACGTTCCCGGCCGGC
ACGCTGTCCGGTGCGCCGAAGGTCCGTGCGATGGAACTGATCGACGAACTCGAGCCGGTCAAGCGCGGGTTGTACGGCGG
CGCCGTCGGCTACCTGTCGTTCTCGGGCGAGATGGATCTCGCGATCGCGATCCGCACGGGCCTGATCCACAACGGCAACC
TGTACGTGCAAGCGGCGGCCGGCGTCGTCGCGGATTCGGTGCCCGAATCCGAATGGCAAGAGACCGAGAACAAGGCGCGC
GCAGTGCTGCGTGCGGCCGAACAGGTCCAGGACGGCCTCGATAGCGACTTCTGA

Upstream 100 bases:

>100_bases
ACAGGCACTTCTCGATCGACCGCCCTTTCGCCGACGGCACCCCGCGCAGCGTCAAGTGATCCCTGGCGCACGCGCCGCTC
GTCCCGAACAGGACCGGAAC

Downstream 100 bases:

>100_bases
CCGGAGACTGACCATGCTGCTCATGATCGACAACTACGATTCGTTTACCTACAACCTGGTCCAGTACTTCGGCGAACTCG
GCGAGGACGTGCGCACCTAC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTELEFQSLANEGYNRIPLIAEALADLETPLSLYLKLAQPERSGANSFLLESVVGGERFGRYSFIGLPARTLVRTRNGVS
EVVRDGQVVETHDGDPFQFIESFQARFKVAQRPGLPRFCGGLAGYFGYDAVRYIEKKLANTTPRDDLGLPDIQLLLTEEV
AVIDNLAGKLYLIIYADPSQAEAYTKAKQRLRELKQRLRTTVQPPVTSASVRTETFREFKKDDYLAAVRQAKEYIAAGEL
MQIQVGQRLTKPYRDNPLSLYRALRSLNPSPYMYYYNFGDFHVVGASPEILVRQEKRGEDQIVTIRPLAGTRPRGNTPER
DAELATELLNDPKEIAEHVMLIDLARNDVGRIAEIGSVHVTDQMVIEKYSHVQHIVSSVEGKLKPGMTNYDVLRATFPAG
TLSGAPKVRAMELIDELEPVKRGLYGGAVGYLSFSGEMDLAIAIRTGLIHNGNLYVQAAAGVVADSVPESEWQETENKAR
AVLRAAEQVQDGLDSDF

Sequences:

>Translated_497_residues
MTELEFQSLANEGYNRIPLIAEALADLETPLSLYLKLAQPERSGANSFLLESVVGGERFGRYSFIGLPARTLVRTRNGVS
EVVRDGQVVETHDGDPFQFIESFQARFKVAQRPGLPRFCGGLAGYFGYDAVRYIEKKLANTTPRDDLGLPDIQLLLTEEV
AVIDNLAGKLYLIIYADPSQAEAYTKAKQRLRELKQRLRTTVQPPVTSASVRTETFREFKKDDYLAAVRQAKEYIAAGEL
MQIQVGQRLTKPYRDNPLSLYRALRSLNPSPYMYYYNFGDFHVVGASPEILVRQEKRGEDQIVTIRPLAGTRPRGNTPER
DAELATELLNDPKEIAEHVMLIDLARNDVGRIAEIGSVHVTDQMVIEKYSHVQHIVSSVEGKLKPGMTNYDVLRATFPAG
TLSGAPKVRAMELIDELEPVKRGLYGGAVGYLSFSGEMDLAIAIRTGLIHNGNLYVQAAAGVVADSVPESEWQETENKAR
AVLRAAEQVQDGLDSDF
>Mature_496_residues
TELEFQSLANEGYNRIPLIAEALADLETPLSLYLKLAQPERSGANSFLLESVVGGERFGRYSFIGLPARTLVRTRNGVSE
VVRDGQVVETHDGDPFQFIESFQARFKVAQRPGLPRFCGGLAGYFGYDAVRYIEKKLANTTPRDDLGLPDIQLLLTEEVA
VIDNLAGKLYLIIYADPSQAEAYTKAKQRLRELKQRLRTTVQPPVTSASVRTETFREFKKDDYLAAVRQAKEYIAAGELM
QIQVGQRLTKPYRDNPLSLYRALRSLNPSPYMYYYNFGDFHVVGASPEILVRQEKRGEDQIVTIRPLAGTRPRGNTPERD
AELATELLNDPKEIAEHVMLIDLARNDVGRIAEIGSVHVTDQMVIEKYSHVQHIVSSVEGKLKPGMTNYDVLRATFPAGT
LSGAPKVRAMELIDELEPVKRGLYGGAVGYLSFSGEMDLAIAIRTGLIHNGNLYVQAAAGVVADSVPESEWQETENKARA
VLRAAEQVQDGLDSDF

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=379, Percent_Identity=41.4248021108179, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788114, Length=445, Percent_Identity=31.4606741573034, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1786809, Length=225, Percent_Identity=30.6666666666667, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082077, Length=275, Percent_Identity=28, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6320935, Length=465, Percent_Identity=40.6451612903226, Blast_Score=316, Evalue=5e-87,
Organism=Saccharomyces cerevisiae, GI6324361, Length=500, Percent_Identity=25.8, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 55270; Mature: 55139

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELEFQSLANEGYNRIPLIAEALADLETPLSLYLKLAQPERSGANSFLLESVVGGERFG
CCCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHEEECCCCCCCCHHHHHHHHHCCHHCC
RYSFIGLPARTLVRTRNGVSEVVRDGQVVETHDGDPFQFIESFQARFKVAQRPGLPRFCG
CEEEECCCHHHHHHHHCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
GLAGYFGYDAVRYIEKKLANTTPRDDLGLPDIQLLLTEEVAVIDNLAGKLYLIIYADPSQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCH
AEAYTKAKQRLRELKQRLRTTVQPPVTSASVRTETFREFKKDDYLAAVRQAKEYIAAGEL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MQIQVGQRLTKPYRDNPLSLYRALRSLNPSPYMYYYNFGDFHVVGASPEILVRQEKRGED
EEEHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEECCCCCCC
QIVTIRPLAGTRPRGNTPERDAELATELLNDPKEIAEHVMLIDLARNDVGRIAEIGSVHV
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEC
TDQMVIEKYSHVQHIVSSVEGKLKPGMTNYDVLRATFPAGTLSGAPKVRAMELIDELEPV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHH
KRGLYGGAVGYLSFSGEMDLAIAIRTGLIHNGNLYVQAAAGVVADSVPESEWQETENKAR
HHHHCCCEEEEEEECCCCEEEEEEEECEEECCCEEEEECCCHHHCCCCCHHHHHHHHHHH
AVLRAAEQVQDGLDSDF
HHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TELEFQSLANEGYNRIPLIAEALADLETPLSLYLKLAQPERSGANSFLLESVVGGERFG
CCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHEEECCCCCCCCHHHHHHHHHCCHHCC
RYSFIGLPARTLVRTRNGVSEVVRDGQVVETHDGDPFQFIESFQARFKVAQRPGLPRFCG
CEEEECCCHHHHHHHHCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
GLAGYFGYDAVRYIEKKLANTTPRDDLGLPDIQLLLTEEVAVIDNLAGKLYLIIYADPSQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCH
AEAYTKAKQRLRELKQRLRTTVQPPVTSASVRTETFREFKKDDYLAAVRQAKEYIAAGEL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MQIQVGQRLTKPYRDNPLSLYRALRSLNPSPYMYYYNFGDFHVVGASPEILVRQEKRGED
EEEHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEECCCCCCC
QIVTIRPLAGTRPRGNTPERDAELATELLNDPKEIAEHVMLIDLARNDVGRIAEIGSVHV
CEEEEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHCCCEEC
TDQMVIEKYSHVQHIVSSVEGKLKPGMTNYDVLRATFPAGTLSGAPKVRAMELIDELEPV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEECCCCCCCCCCCHHHHHHHHHHHHH
KRGLYGGAVGYLSFSGEMDLAIAIRTGLIHNGNLYVQAAAGVVADSVPESEWQETENKAR
HHHHCCCEEEEEEECCCCEEEEEEEECEEECCCEEEEECCCHHHCCCCCHHHHHHHHHHH
AVLRAAEQVQDGLDSDF
HHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]