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The map label for this gene is hisF [H]

Identifier: 78065006

GI number: 78065006

Start: 395094

End: 395867

Strand: Direct

Name: hisF [H]

Synonym: Bcep18194_A3529

Alternate gene names: 78065006

Gene position: 395094-395867 (Clockwise)

Preceding gene: 78065005

Following gene: 78065007

Centisome position: 10.7

GC content: 69.12

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGCCTGGACGTGACTGCCGGGCGTGTCGTCAAGGGCGTCAACTTCGTCGAGCT
GCGCGACGCCGGCGACCCCGTCGAGATCGCCCGCCGCTACGACGACCAGGGCGCCGACGAACTGACGTTCCTCGACATCA
CCGCGACGTCCGACCAGCGCGACCTGATTCTGCCGATCATCGAAGCCGTCGCGTCGCAGGTCTTCATTCCGCTGACCGTC
GGCGGCGGCGTGCGCGCCGTCGAGGACGTGCGACGCCTGCTGAACGCGGGCGCGGACAAGGTCAGCATGAATTCGTCGGC
GGTCGCGAACCCGCAGCTCGTGCGCGACGCGGCCGACAAGTACGGCTCGCAATGCATCGTCGTCGCGATCGACGCGAAGC
GCGTGTCGGCCGACGGCGAGACGCCGCGCTGGGAAGTCTTCACGCATGGCGGCCGCAAGAACACGGGCCTCGACGCGATC
GAATGGGCGCGCAAGATGGCCGAGCTCGGCGCGGGCGAGATCCTGCTCACGAGCATGGACCGCGACGGCACCAAGTCGGG
CTTCGACCTCGCGCTCACGCGCGGCGTGTCGGACGCGGTGCCGGTGCCGGTGATCGCATCGGGCGGGGTCGGCTCGCTGC
AGCACCTGGCCGACGGCATCAAGGACGGTCGCGCCGACGCGGTGCTGGCCGCGAGTATCTTCCACTACGGCGAGCACACG
GTCGGCGAGGCAAAGCGCTTCATGGCCGACCAGGGCATCCCGGTGAGGCTGTGA

Upstream 100 bases:

>100_bases
TGTCCCGCTCGCCGGGGCTGCCTGCAGGCGGCCCCGACCGGAAGCCTCGCGCGAGGCTTTCCGTAACCGGCCGTCCCATC
GCGGCATTTGGCGCAACATC

Downstream 100 bases:

>100_bases
TGAATACGGAAACGAATGCATTGCCCGCGTGGCTCGACAAGGTCCGCTGGGACGACAACGGCCTCGTGCCGGTGATCGCG
CAGGAAGCGTCGACGAACGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAI
EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
VGEAKRFMADQGIPVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAI
EWARKMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
VGEAKRFMADQGIPVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAIE
WARKMAELGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHTV
GEAKRFMADQGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=43.2432432432432, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=31.404958677686, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.126582278481, Blast_Score=172, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27261; Mature: 27130

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAIEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
VGEAKRFMADQGIPVRL
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDDQGADELTFLDITATSDQR
CCHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSADGETPRWEVFTHGGRKNTGLDAIEWARKMAELGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQHLADGIKDGRADAVLAASIFHYGEHT
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
VGEAKRFMADQGIPVRL
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA