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The map label for this gene is tuf

Identifier: 78064922

GI number: 78064922

Start: 320417

End: 321607

Strand: Direct

Name: tuf

Synonym: Bcep18194_A3445

Alternate gene names: 78064922

Gene position: 320417-321607 (Clockwise)

Preceding gene: 78064921

Following gene: 78064923

Centisome position: 8.67

GC content: 61.63

Gene sequence:

>1191_bases
ATGGCAAAAGGTAAATTCGAGCGGACCAAGCCGCACGTGAACGTTGGTACGATTGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCAGCGATCACGACGGTTCTGACGAAGAAGTTCGGCGGCGAAGCGAAGGCATACGACCAGATCGACGCGGCAC
CGGAAGAAAAGGCGCGCGGCATCACGATCAACACGGCGCACGTCGAGTACGAAACGGCTAACCGCCACTACGCACACGTC
GACTGCCCGGGCCACGCTGACTATGTGAAGAACATGATCACGGGCGCAGCGCAGATGGACGGCGCGATCCTGGTTTGCTC
GGCAGCTGACGGCCCGATGCCGCAGACGCGTGAGCACATCCTGCTGGCGCGTCAGGTTGGCGTTCCGTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAACTGCTCGAGCTGGTCGAGATGGAAGTTCGCGAACTCCTGTCGAAG
TACGACTTCCCGGGCGACGACACGCCGATCGTGAAGGGTTCGGCCAAGCTGGCGCTGGAAGGCGACACGGGCGAGCTGGG
CGAAGTGGCGATCATGAGCCTGGCCGACGCGCTGGACACGTACATCCCGACGCCGGAGCGTGCAGTTGACGGCGCGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGCCGTGGTACGGTCGTGACGGGTCGCGTTGAGCGCGGCATCGTGAAG
GTCGGCGAAGAAATCGAAATCGTCGGTATCAAGCCGACGGTGAAGACGACCTGCACGGGCGTTGAAATGTTCCGCAAGCT
GCTGGACCAAGGTCAAGCAGGCGACAACGTTGGTATCCTGCTGCGCGGCACGAAGCGTGAAGACGTGGAGCGTGGCCAGG
TTCTGGCGAAGCCGGGTTCGATCACGCCGCACACGCACTTCACGGCTGAAGTGTACGTGCTGAGCAAGGACGAAGGCGGC
CGCCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGCTCGATCGAGCTGCC
GAAGGACAAGGAAATGGTGATGCCGGGCGACAACGTGTCGATCACGGTGAAGCTGATTGCACCGATCGCGATGGAAGAAG
GTCTGCGCTTCGCAATCCGCGAAGGCGGCCGTACGGTCGGCGCCGGCGTCGTCGCCAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
GCACTACGCTGAAGCTCCGAAGAACGTTGCAGAAGCGATCATCAGCGCGAAGACGAAGTAATTCGTTATCACAATCGATT
ATTTTTGAAAGAAGAGAATC

Downstream 100 bases:

>100_bases
TATCGACGATCCGCGGATCCCGACGGGGTCCGCGATTCCGCGGTATGCAGTCCGTACCGTCGAAACCGGGTGTCCAGCTT
GTGCTGGCACCCGGTTTTGT

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIVKGSAKLALEGDTGELGEVAIMSLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIVKGSAKLALEGDTGELGEVAIMSLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIVK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDDTPIVKGSAKLALEGDTGELGEVAIMSLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIVKV
GEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSITPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.1065989847716, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.9303944315545, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.9303944315545, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.5714285714286, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.185520361991, Blast_Score=151, Evalue=8e-37,
Organism=Homo sapiens, GI194018522, Length=432, Percent_Identity=28.0092592592593, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194097354, Length=432, Percent_Identity=28.0092592592593, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI46094014, Length=432, Percent_Identity=27.7777777777778, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI194018520, Length=432, Percent_Identity=28.0092592592593, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI54607086, Length=330, Percent_Identity=28.4848484848485, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=27.9850746268657, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.1111111111111, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=26.3157894736842, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=81.2658227848101, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=81.2658227848101, Blast_Score=636, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=30.4985337243402, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=274, Percent_Identity=30.6569343065693, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789108, Length=335, Percent_Identity=28.0597014925373, Blast_Score=85, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.8535980148883, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.702842377261, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=453, Percent_Identity=30.4635761589404, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=453, Percent_Identity=30.4635761589404, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.5714285714286, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=32.7272727272727, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=26.879271070615, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=26.8348623853211, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=29.5454545454545, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=37.4149659863946, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=295, Percent_Identity=26.7796610169492, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=243, Percent_Identity=25.1028806584362, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=534, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=442, Percent_Identity=30.5429864253394, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=442, Percent_Identity=30.5429864253394, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.7619047619048, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=358, Percent_Identity=26.2569832402235, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=28.3018867924528, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=193, Percent_Identity=29.5336787564767, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=27.7310924369748, Blast_Score=63, Evalue=1e-10,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.5241730279898, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.5241730279898, Blast_Score=487, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.0362694300518, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.2410714285714, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.2410714285714, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.7946428571429, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=28.2110091743119, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI17137380, Length=338, Percent_Identity=31.3609467455621, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=29.9674267100977, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.2527881040892, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.2527881040892, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 42935; Mature: 42804

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTKKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIVKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDTGELGEVAIMSLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECHHHCCHHC
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTKKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIVKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDTGELGEVAIMSLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIVK
CCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECHHHCCHHC
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
ITPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA