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The map label for this gene is rffA [H]

Identifier: 78064811

GI number: 78064811

Start: 185876

End: 187033

Strand: Reverse

Name: rffA [H]

Synonym: Bcep18194_A3334

Alternate gene names: 78064811

Gene position: 187033-185876 (Counterclockwise)

Preceding gene: 78064812

Following gene: 78064810

Centisome position: 5.06

GC content: 68.22

Gene sequence:

>1158_bases
ATGTTCGGCCCACCCAATCTCGACTGCATTCCATTCGGCCGCCCGTTCGTGGTCGGCAAGGAGCTCTACTACATCGCGAA
AGCGGTCGAGCAGGGCGGCCTCGCCGGCGACCAGGCGTTCACGAAGCTCTGCCACCGCTGGCTGGAAGCGCGCATCGGCT
GCCAGCGCGCGCTGCTCACGCACTCGTGCACGGCCGCGCTCGAGATGGCCGCGATCCTCGCCGACGTGCAGCCCGGCGAC
GAAGTGATCATGCCGTCCTACACGTTCGTGTCGACCGCCAACGCGTTCGTGCTGCGCGGCGCGACGCCGGTGTTCGTCGA
CATCCGCCGCGACACGCTGAACCTTGACGAGACGCAGATCGCCGCCGCGATCACCCAGCGCACGCGCGCGATCGTGCCGG
TGCACTACGCGGGCGTCGCATGCGACATGGACGTGATCGGGCAGCTCGCGGCGGACCACGACCTGTGGGTGATCGAGGAC
GCCGCCCAGGCGCTGCAGTCGACGTGGAACGGCAAGCCGCTCGGCAGCCTCGGCCACCTCGCGTGCCTCAGCTTCCACGA
GACCAAGAACGTGATCTCGGGCGAAGGCGGCGCGCTGCTGGTCAACGACCCGCGCCTCGTCGAGCGCGCGGAGATCATCC
GCGAGAAGGGCACCAACCGCAGTCAGTTCTTCCGCGGGCAGGTGGACAAGTACACATGGGTCGACGTCGGCTCGTCGTTC
CTGCCGGGCGAGCTGATCGCGGCATTCCTGTACGCGCAGTTCGAGCACGCGGACGTGATCACCGCGCAGCGGCGCGCGAC
CGTGCAGCGCTACCAGGACGCGCTGCAGCCGCTGCACGCGGACGGGCTGATCGAGCTGCCCGCGATTTCGCTCGCCGAGC
GCGGCAACGGCCACATGTTCTATTTCCTCGCACGCGACCTCGCCGAACGCACCGATCTGCTGCAGCAGATCCGCGCGGTG
GGCGTGAACGCGGTGTTCCATTACGTGCCGCTGCACAGTTCGCCGGCCGGCCAGCGCTACGGGCGCTGCGGGTCCGGCAT
GGCGGTGACGGACCTCGTCGCCGACCGGCTCGTCCGGCTGCCGCTGTACCACGACATGACCGAAGCCGAACAGGATCGCA
TCCTCGAAGTGGTCTTCGATTTCTACCGCACCCGATAA

Upstream 100 bases:

>100_bases
ACGACGGCCCTGCGCGCTCGCGCGCTGCCGTCCGGCAGGACGCCTGAACGGCGCCGCGCCCGCGCCGCCGATCGCCCGTC
ACTTTCCGAGGAGCCTGACC

Downstream 100 bases:

>100_bases
CGATGACGACTCCCAAACCTGGCTTGGCGCACAGCGGTTCGCGCATCTTTACGCGACTCGACCACCCGTACTACATACTC
GCGCCGAATTTCCGGCAAAC

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MFGPPNLDCIPFGRPFVVGKELYYIAKAVEQGGLAGDQAFTKLCHRWLEARIGCQRALLTHSCTAALEMAAILADVQPGD
EVIMPSYTFVSTANAFVLRGATPVFVDIRRDTLNLDETQIAAAITQRTRAIVPVHYAGVACDMDVIGQLAADHDLWVIED
AAQALQSTWNGKPLGSLGHLACLSFHETKNVISGEGGALLVNDPRLVERAEIIREKGTNRSQFFRGQVDKYTWVDVGSSF
LPGELIAAFLYAQFEHADVITAQRRATVQRYQDALQPLHADGLIELPAISLAERGNGHMFYFLARDLAERTDLLQQIRAV
GVNAVFHYVPLHSSPAGQRYGRCGSGMAVTDLVADRLVRLPLYHDMTEAEQDRILEVVFDFYRTR

Sequences:

>Translated_385_residues
MFGPPNLDCIPFGRPFVVGKELYYIAKAVEQGGLAGDQAFTKLCHRWLEARIGCQRALLTHSCTAALEMAAILADVQPGD
EVIMPSYTFVSTANAFVLRGATPVFVDIRRDTLNLDETQIAAAITQRTRAIVPVHYAGVACDMDVIGQLAADHDLWVIED
AAQALQSTWNGKPLGSLGHLACLSFHETKNVISGEGGALLVNDPRLVERAEIIREKGTNRSQFFRGQVDKYTWVDVGSSF
LPGELIAAFLYAQFEHADVITAQRRATVQRYQDALQPLHADGLIELPAISLAERGNGHMFYFLARDLAERTDLLQQIRAV
GVNAVFHYVPLHSSPAGQRYGRCGSGMAVTDLVADRLVRLPLYHDMTEAEQDRILEVVFDFYRTR
>Mature_385_residues
MFGPPNLDCIPFGRPFVVGKELYYIAKAVEQGGLAGDQAFTKLCHRWLEARIGCQRALLTHSCTAALEMAAILADVQPGD
EVIMPSYTFVSTANAFVLRGATPVFVDIRRDTLNLDETQIAAAITQRTRAIVPVHYAGVACDMDVIGQLAADHDLWVIED
AAQALQSTWNGKPLGSLGHLACLSFHETKNVISGEGGALLVNDPRLVERAEIIREKGTNRSQFFRGQVDKYTWVDVGSSF
LPGELIAAFLYAQFEHADVITAQRRATVQRYQDALQPLHADGLIELPAISLAERGNGHMFYFLARDLAERTDLLQQIRAV
GVNAVFHYVPLHSSPAGQRYGRCGSGMAVTDLVADRLVRLPLYHDMTEAEQDRILEVVFDFYRTR

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=374, Percent_Identity=57.2192513368984, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI145693159, Length=389, Percent_Identity=29.8200514138817, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 42558; Mature: 42558

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGPPNLDCIPFGRPFVVGKELYYIAKAVEQGGLAGDQAFTKLCHRWLEARIGCQRALLT
CCCCCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HSCTAALEMAAILADVQPGDEVIMPSYTFVSTANAFVLRGATPVFVDIRRDTLNLDETQI
HHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCCEEEEECCCEEEEEEECCCCCCCHHHH
AAAITQRTRAIVPVHYAGVACDMDVIGQLAADHDLWVIEDAAQALQSTWNGKPLGSLGHL
HHHHHHHHCEEEEEEECCEEECHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCHHHH
ACLSFHETKNVISGEGGALLVNDPRLVERAEIIREKGTNRSQFFRGQVDKYTWVDVGSSF
HHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCC
LPGELIAAFLYAQFEHADVITAQRRATVQRYQDALQPLHADGLIELPAISLAERGNGHMF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHCCCCCEE
YFLARDLAERTDLLQQIRAVGVNAVFHYVPLHSSPAGQRYGRCGSGMAVTDLVADRLVRL
EHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHC
PLYHDMTEAEQDRILEVVFDFYRTR
CHHHCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGPPNLDCIPFGRPFVVGKELYYIAKAVEQGGLAGDQAFTKLCHRWLEARIGCQRALLT
CCCCCCCCEEECCCCEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HSCTAALEMAAILADVQPGDEVIMPSYTFVSTANAFVLRGATPVFVDIRRDTLNLDETQI
HHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCCCEEEEECCCEEEEEEECCCCCCCHHHH
AAAITQRTRAIVPVHYAGVACDMDVIGQLAADHDLWVIEDAAQALQSTWNGKPLGSLGHL
HHHHHHHHCEEEEEEECCEEECHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCHHHH
ACLSFHETKNVISGEGGALLVNDPRLVERAEIIREKGTNRSQFFRGQVDKYTWVDVGSSF
HHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCC
LPGELIAAFLYAQFEHADVITAQRRATVQRYQDALQPLHADGLIELPAISLAERGNGHMF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCHHHHHCCCCCEE
YFLARDLAERTDLLQQIRAVGVNAVFHYVPLHSSPAGQRYGRCGSGMAVTDLVADRLVRL
EHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHHHC
PLYHDMTEAEQDRILEVVFDFYRTR
CHHHCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]