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The map label for this gene is argT [H]

Identifier: 78064777

GI number: 78064777

Start: 141704

End: 142492

Strand: Direct

Name: argT [H]

Synonym: Bcep18194_A3300

Alternate gene names: 78064777

Gene position: 141704-142492 (Clockwise)

Preceding gene: 78064776

Following gene: 78064778

Centisome position: 3.84

GC content: 66.67

Gene sequence:

>789_bases
ATGAAGCACACGGCCACCCTGAAACTCGCGTTCGCACTCGCGTGCGCAATCGGCTCGGGCGCGGCGCTCGCCGATGCGCA
GACGCTGCGCTTCGGCCTCGAGGCGCAATACCCGCCGTTCGAATCGAAAGCGCCGAACGGCGACCTGCAGGGCTTCGACA
TCGACGTCGGCAACGCGGTCTGCAAGACGGCGCAGTTGTCGTGCAAATGGGTCGAGACGTCGTTCGACGGCCTGATTCCC
GCGCTGCAGGGCCGCAAGTTCGACGCGATCAATTCGGCGATGAACGCGACCGAGCAGCGGCGCCAGGCGATCGATTTCAC
GACGATCATCTATCGCGTGCCGACCCAGCTGATCGCGCGTACGGGCAGCGGCCTGCTGCCGACGCCGGAATCGCTGAAGG
GCAAGCGTGTCGGCGTGCTGCAGGCGTCGATCCAGGAAACCTTCGCGAAGGCGCACTGGGAGCCGGCCGGCGTCGCGGTC
GTCGCGTACCAGGACCAGAACCAGGCGTACGCGGACCTCGTGTCGGGCCGTCTCGACGCGACGCTCGTGCTCGCACCGTC
CGGCCAGCGCGGCTTCCTGTCGCGCCCGGACGGCAAGGGCTTCTCGTTCGTCGGCCAGCCCGTGCGCGACGACAAGATCC
TCGGCAGCGGCATCGCATTCGGGCTGCGCAAAGGCGACAGCGCGCTGAAGGCGAAACTCGATGCGGCGATCGACAAGCTG
AAGGCGGACGGCACGGTGAAGTCGCTCGGCCTGAAGTATTTCGGCGATATCGACATCTCGGCGAAGTAA

Upstream 100 bases:

>100_bases
CGCGACGCTGGTCGAAGGCGCGCGGCGGCTGCGTTCGCTGTAAAGCCCGTACGTAAACCAGCGACGGGAGCGTCGCATCG
ACCAGACACAGGAGATCACG

Downstream 100 bases:

>100_bases
GCGGAGCCGCACGCGATGCAGAACCGGAACACGGCCGCCGCGGGTGGCGAACAGGGCGACGCATCGCTGTCGGGCGACGC
GATCGATGCCGTGCTCGTCG

Product: ABC amino acid transporter, periplasmic ligand binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKHTATLKLAFALACAIGSGAALADAQTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAVCKTAQLSCKWVETSFDGLIP
ALQGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAV
VAYQDQNQAYADLVSGRLDATLVLAPSGQRGFLSRPDGKGFSFVGQPVRDDKILGSGIAFGLRKGDSALKAKLDAAIDKL
KADGTVKSLGLKYFGDIDISAK

Sequences:

>Translated_262_residues
MKHTATLKLAFALACAIGSGAALADAQTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAVCKTAQLSCKWVETSFDGLIP
ALQGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAV
VAYQDQNQAYADLVSGRLDATLVLAPSGQRGFLSRPDGKGFSFVGQPVRDDKILGSGIAFGLRKGDSALKAKLDAAIDKL
KADGTVKSLGLKYFGDIDISAK
>Mature_262_residues
MKHTATLKLAFALACAIGSGAALADAQTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAVCKTAQLSCKWVETSFDGLIP
ALQGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIARTGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAV
VAYQDQNQAYADLVSGRLDATLVLAPSGQRGFLSRPDGKGFSFVGQPVRDDKILGSGIAFGLRKGDSALKAKLDAAIDKL
KADGTVKSLGLKYFGDIDISAK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=45.8498023715415, Blast_Score=224, Evalue=4e-60,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=45.0643776824034, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=32.6612903225806, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1787085, Length=230, Percent_Identity=33.0434782608696, Blast_Score=134, Evalue=6e-33,
Organism=Escherichia coli, GI1788228, Length=231, Percent_Identity=30.3030303030303, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787031, Length=258, Percent_Identity=29.0697674418605, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27805; Mature: 27805

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTATLKLAFALACAIGSGAALADAQTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAV
CCCCHHHHHHHHHHHHHCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
CKTAQLSCKWVETSFDGLIPALQGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIAR
HHHHHCEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAVVAYQDQNQAYADLVSGRLDA
CCCCCCCCCHHCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCCE
TLVLAPSGQRGFLSRPDGKGFSFVGQPVRDDKILGSGIAFGLRKGDSALKAKLDAAIDKL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHHH
KADGTVKSLGLKYFGDIDISAK
CCCCCHHHCCCEEECCEEECCC
>Mature Secondary Structure
MKHTATLKLAFALACAIGSGAALADAQTLRFGLEAQYPPFESKAPNGDLQGFDIDVGNAV
CCCCHHHHHHHHHHHHHCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCHHH
CKTAQLSCKWVETSFDGLIPALQGRKFDAINSAMNATEQRRQAIDFTTIIYRVPTQLIAR
HHHHHCEEEEEECCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSGLLPTPESLKGKRVGVLQASIQETFAKAHWEPAGVAVVAYQDQNQAYADLVSGRLDA
CCCCCCCCCHHCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHCCCCCE
TLVLAPSGQRGFLSRPDGKGFSFVGQPVRDDKILGSGIAFGLRKGDSALKAKLDAAIDKL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHHH
KADGTVKSLGLKYFGDIDISAK
CCCCCHHHCCCEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]