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The map label for this gene is oprM [H]

Identifier: 78064709

GI number: 78064709

Start: 63863

End: 65434

Strand: Direct

Name: oprM [H]

Synonym: Bcep18194_A3232

Alternate gene names: 78064709

Gene position: 63863-65434 (Clockwise)

Preceding gene: 78064708

Following gene: 78064718

Centisome position: 1.73

GC content: 72.07

Gene sequence:

>1572_bases
ATGCCGTACGCCTCCCTCCCGCGCGCCCTTCGCCCGCTGAGCGCCGCCGTCGCCGTCGCGACGGCCGTGCTGCTCGCCGG
CTGCGCCGTCGGGCCCGACTATCACCGGCCCGACACGTCGATCCCCGCCGCCTACAAGGAAGCGCCGGCCGGCTGGAAGG
TCGCGCAGCCCGCCGACCGCACCGACCGCGGCGCGTGGTGGACGGTCTACAACGATCCGCAGCTCGATGCGCTGATCGGC
AAGCTCAACGCGTCGAACCAGACCATCGCGCAATCGGCGGCCGCGTACCGGCAGGCGCGCGCACTCGTCACCGAGGCGCG
TGCCGCGTACTTCCCGACCGTCGGGCTGACCGCGTCGGGCTCACGCGCCCGCACGCCGCGCACGTCGGTGTCGTCGGGTT
CGTCGTCGAGCTTCAGCAGCGGTTCGTCCGGCTCGATCAGCAACAGCTATAGCGTCGGCCTCGACGCGAGCTGGGAACCC
GACCTGTGGGGCAAGGTGAGCCGTACCGTCAGCGCGCAGCGCGCCGGCGAAGCCGCCGCCGCGGCCGATCTCGCGAATGC
GCGGCTGTCGCAGCAGGCGCTGCTCGCGCAAACCTACTTCCAGCTGCGCACGTCCGATGCGCTGCAGAAGCTGCTCGACG
ACACCGTGAAGTCGTACGAACAGTCGCTGAAACTCACGCAGAACCAGTACGCGCAGGGCGTCGCGGCTCGCGCGGACGTG
ATCCAGGCGCAGACGCAGCTACAGAGCGCGCAGGCCGCGTCGATCGACAACGGCGTGGCCCGCGCGCAGTACGAGCATGC
GATCGCGACGCTGATCGGCGAGCCGGCGTCGACCTTCTCGCTGTCGCCAAGCCCGCTCACGGCCGAGCCGCCGATCACGC
CGGTCGACGTGCCGTCCGCGATCCTCGAGCGCCGGCCCGACATCGCCGCGGCCGAGCGCCGCGCGGCGGCCGCGAATGAG
CAGATCGGCGTCGCGATCGCCGCGTTCTTCCCGACGCTCACGCTGTCGGCCACCGGCGGCGTCCAGAGCTCGGTGTGGTC
GCAGTTGTTCACGCTGCCGGCGCGCTTCTGGACGGTCGGCCCGCAACTCGCGGCCACGCTGTTCGACGCCGGGCTGCGCG
CCGCGCAGACCGACGCCGCGCGCGCGACCTACGACCAGGACGTCGCCGCGTACCGCCTCGCGGTGCTCACCGCGTTCCAG
GACGTCGAGGACAACCTCGCGTCGCAACGCATCCTTGCGCAGGAAATCGATGTGCAGCGGCAGGCCGTCGACAGCGCCGA
GCATTCGCTCGCGATCGTCACGAACCAGTACAAGGCCGGCACGGTCGCCTACCTGAACGTGCTGACCGCGCAGACCACCG
CGTTCACCGCGCAGCAGAAGCTCGCGACGATTGCCGGGCAGCGGATGACGTCGTCGGTCGGGCTCGTGAAGGCGCTCGGC
GGCGGCTGGAGCGCAGCGGAGATGGCGCGCGAGAACGGCGACATGGCGGCACCGGCACCCGTGCCGGCATCCGGTGCGGT
CGCGCCCGCGGCCACCGCACCGTCGGTCGCCGCGCCGCTCGCGCAGAAGTAG

Upstream 100 bases:

>100_bases
CAGCTTTCCCGCCGTCAGTTACCATTTGTCCGCCAGCGGCACGACGACCGCATCGCGCGACGCCGCTCGCCGGACGTCGC
GCCCAGACAGGAAAACCACC

Downstream 100 bases:

>100_bases
GAAGGAATGCGCGACGCGCGCGTCAGCGCTGCGCGTCGCCGAAGATCAGCGACACCTCGTTGTTGCCGATCGGGTGGCCG
AGCAGGTTCAGCAGCCCTGC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MPYASLPRALRPLSAAVAVATAVLLAGCAVGPDYHRPDTSIPAAYKEAPAGWKVAQPADRTDRGAWWTVYNDPQLDALIG
KLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASGSRARTPRTSVSSGSSSSFSSGSSGSISNSYSVGLDASWEP
DLWGKVSRTVSAQRAGEAAAAADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYEQSLKLTQNQYAQGVAARADV
IQAQTQLQSAQAASIDNGVARAQYEHAIATLIGEPASTFSLSPSPLTAEPPITPVDVPSAILERRPDIAAAERRAAAANE
QIGVAIAAFFPTLTLSATGGVQSSVWSQLFTLPARFWTVGPQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQ
DVEDNLASQRILAQEIDVQRQAVDSAEHSLAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMTSSVGLVKALG
GGWSAAEMARENGDMAAPAPVPASGAVAPAATAPSVAAPLAQK

Sequences:

>Translated_523_residues
MPYASLPRALRPLSAAVAVATAVLLAGCAVGPDYHRPDTSIPAAYKEAPAGWKVAQPADRTDRGAWWTVYNDPQLDALIG
KLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASGSRARTPRTSVSSGSSSSFSSGSSGSISNSYSVGLDASWEP
DLWGKVSRTVSAQRAGEAAAAADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYEQSLKLTQNQYAQGVAARADV
IQAQTQLQSAQAASIDNGVARAQYEHAIATLIGEPASTFSLSPSPLTAEPPITPVDVPSAILERRPDIAAAERRAAAANE
QIGVAIAAFFPTLTLSATGGVQSSVWSQLFTLPARFWTVGPQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQ
DVEDNLASQRILAQEIDVQRQAVDSAEHSLAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMTSSVGLVKALG
GGWSAAEMARENGDMAAPAPVPASGAVAPAATAPSVAAPLAQK
>Mature_522_residues
PYASLPRALRPLSAAVAVATAVLLAGCAVGPDYHRPDTSIPAAYKEAPAGWKVAQPADRTDRGAWWTVYNDPQLDALIGK
LNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASGSRARTPRTSVSSGSSSSFSSGSSGSISNSYSVGLDASWEPD
LWGKVSRTVSAQRAGEAAAAADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYEQSLKLTQNQYAQGVAARADVI
QAQTQLQSAQAASIDNGVARAQYEHAIATLIGEPASTFSLSPSPLTAEPPITPVDVPSAILERRPDIAAAERRAAAANEQ
IGVAIAAFFPTLTLSATGGVQSSVWSQLFTLPARFWTVGPQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQD
VEDNLASQRILAQEIDVQRQAVDSAEHSLAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMTSSVGLVKALGG
GWSAAEMARENGDMAAPAPVPASGAVAPAATAPSVAAPLAQK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=481, Percent_Identity=28.8981288981289, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI2367348, Length=494, Percent_Identity=25.9109311740891, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54460; Mature: 54329

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYASLPRALRPLSAAVAVATAVLLAGCAVGPDYHRPDTSIPAAYKEAPAGWKVAQPADR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
TDRGAWWTVYNDPQLDALIGKLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASG
CCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
SRARTPRTSVSSGSSSSFSSGSSGSISNSYSVGLDASWEPDLWGKVSRTVSAQRAGEAAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
AADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYEQSLKLTQNQYAQGVAARADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAQTQLQSAQAASIDNGVARAQYEHAIATLIGEPASTFSLSPSPLTAEPPITPVDVPSA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILERRPDIAAAERRAAAANEQIGVAIAAFFPTLTLSATGGVQSSVWSQLFTLPARFWTVG
HHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCC
PQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQDVEDNLASQRILAQEIDVQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVDSAEHSLAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMTSSVGLVKALG
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGWSAAEMARENGDMAAPAPVPASGAVAPAATAPSVAAPLAQK
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure 
PYASLPRALRPLSAAVAVATAVLLAGCAVGPDYHRPDTSIPAAYKEAPAGWKVAQPADR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
TDRGAWWTVYNDPQLDALIGKLNASNQTIAQSAAAYRQARALVTEARAAYFPTVGLTASG
CCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCC
SRARTPRTSVSSGSSSSFSSGSSGSISNSYSVGLDASWEPDLWGKVSRTVSAQRAGEAAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHCCHHHH
AADLANARLSQQALLAQTYFQLRTSDALQKLLDDTVKSYEQSLKLTQNQYAQGVAARADV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IQAQTQLQSAQAASIDNGVARAQYEHAIATLIGEPASTFSLSPSPLTAEPPITPVDVPSA
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILERRPDIAAAERRAAAANEQIGVAIAAFFPTLTLSATGGVQSSVWSQLFTLPARFWTVG
HHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHCC
PQLAATLFDAGLRAAQTDAARATYDQDVAAYRLAVLTAFQDVEDNLASQRILAQEIDVQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QAVDSAEHSLAIVTNQYKAGTVAYLNVLTAQTTAFTAQQKLATIAGQRMTSSVGLVKALG
HHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GGWSAAEMARENGDMAAPAPVPASGAVAPAATAPSVAAPLAQK
CCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]