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The map label for this gene is hsdM [H]

Identifier: 78064669

GI number: 78064669

Start: 16591

End: 18408

Strand: Direct

Name: hsdM [H]

Synonym: Bcep18194_A3192

Alternate gene names: 78064669

Gene position: 16591-18408 (Clockwise)

Preceding gene: 78064668

Following gene: 78064670

Centisome position: 0.45

GC content: 51.16

Gene sequence:

>1818_bases
ATGGCAACTCTCGAGCAATTCAGAGATTTTCTGGTCACGGATCTGGGTTTCAAAGCCCATCAGATCAATGACTTTAATGG
TGCTGGCGTTGCCACAACGGTTGATGGACTGGACGAGAAAGCAGTGTTTCTGGTGTCAACCAGCGTCGGCAACTGCTATG
CCGCCTTCAGGCATGAAGGCTCGGCAACGCTGAGCGAGTCGGAGGCCAACCGGTACAAAGCCCTTAGCATTCTTATGCCC
AATGGCGCAGCGGCGTATTGCGTCATCGAGGTTGCTGGCCATTTACGCATCTATCTCGTCGAAAAAGGCAATGAGATTCA
CGACGAGATGAATGACTGGGACAACTTTCGCGATGCCGAAGATATGGTGCGCGAATTCAAGCCAGACGTGGTCAACAGCC
TTCAGCAGTGGGCACGGAAAACACTCCACACGGAAAGCAGCCTTTCTCGATTCGTGCAGCTCATGAAGGGCTGCTGGCAG
GACATCTGGGATATTGAGAACAAGCGCAATGACTGGATCTTTGATGAATTTACACGTTTTCTATTTATTAAACTCAATGA
AGACGCAAAGCCCAGCGGAAGCTTTACCACGGCCAAGTTGACGTCGTATAGCGAACAGAACAAACACATGGGTGACAAGG
CCGCCCAGAACTTCATCAACAACCTTTTTGATGACCTGAAGGCGCACCACCCCGAAGTTTTTACCGACGAGAACGAACGA
GTACTCAGCAAGGCGGCAACCGTTGAACGCGTCATTGCCCGTCTTGAAGGTATTAACCTGAAAGATACCCAAGGCGATGT
CCTTGGCCGTGCTTTCGAAATTATGCTGTCTGATACTTTTAAGGGTAAAGACCTGGGACAGTTCTTTACTCCGCGAGAAA
TCGTCGCCTTCATGCTGGACCTGGCAAGGGAAAATCCCGAAGGTCCGGCTTTGGATATCAGCAAGGGCGAGCGGTTTCTA
GACGGCTGCGCGGGGTCAGGCGGCTTCCTCATCGCCGCTTATGAAGATGTATACAAGCATGCCCTTTCGAGCACGATTAG
AGGTGATGAGCGCGAGAACCTCCTTAGGCGCCTCGGGCAGGAAACCTTCTTTGCCTGTGAGATCGAAGAAAAGGCGGCTC
GCCTTGGAAAACTGAACATGATCGTTCACGCCGTCAACGCGCAGAACGCGCAGTGGCTTCACCAGAACTACCTCTACAAC
GAGGAGCGGGGCGGTTTAAAACCGCTTATTGAATATGAAGTTGATTTCGGAGAAGGCAAAAAGAAGCGCCAGATCGGCAG
CAGTTCAATCGATCTTATCTTGACGAACCCACCGTTCGGCAAGTCAGTCAAGACTGAAAATGTTCTTCTTGACTATCAGT
TTGGTCACGAAGTAAAAACCTTCAAGTCTGCAGGCCGTCCACCTGAGAAACGAGCGAAAAACAGTCAGGATAGCGAAGTC
CTTTTCATTGAACACTATCTTCGTACGTTGAAGCCTGGCGGAAAACTGCTCATCGTTCTTCCCGACGGAGTGTTGTCGAA
TGCAACGGCGAAGCCCGTTCGGGATTATATTCGTGAGCACGCCATCATCAAATCCGTGATTTCACTTCCAAGCGAAACCT
TCGCATCGACTGGGACCTCCATTCCGACCAATGTAGTGTTTCTGCAGAAGAAGCGCCCAGGAGATGTTCAAGGCGATATC
TTCATGGCGCGAGCTGACTACGTCGGCCGCCGGGCAAATGGCGACCCGCTCAAGGAAAACGATCTCCCGTTCATTCTTGA
AAAATACCGCGAATGGCAAACGGGAACGCTTGAACTCCAGAAGGAGGATAGCGAGTGA

Upstream 100 bases:

>100_bases
GAATCCGAACAAAGTACAAGGTAATGACCATGGCGGTCTTACCCCTGAAGAAACAGTCGTTCCTGTGGCTGTTTACATTA
CGCGTGGAGCAAAGTTGTAA

Downstream 100 bases:

>100_bases
GCGAGCTCTACGTAGGAAAACGTCTCGACCCAAATGGGATGCCAGGAGCGCCGTATCTTCACAAACTAAGTGACCTTATC
ACTCACACTTTCATTTGTGG

Product: N-6 DNA methylase

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 605; Mature: 604

Protein sequence:

>605_residues
MATLEQFRDFLVTDLGFKAHQINDFNGAGVATTVDGLDEKAVFLVSTSVGNCYAAFRHEGSATLSESEANRYKALSILMP
NGAAAYCVIEVAGHLRIYLVEKGNEIHDEMNDWDNFRDAEDMVREFKPDVVNSLQQWARKTLHTESSLSRFVQLMKGCWQ
DIWDIENKRNDWIFDEFTRFLFIKLNEDAKPSGSFTTAKLTSYSEQNKHMGDKAAQNFINNLFDDLKAHHPEVFTDENER
VLSKAATVERVIARLEGINLKDTQGDVLGRAFEIMLSDTFKGKDLGQFFTPREIVAFMLDLARENPEGPALDISKGERFL
DGCAGSGGFLIAAYEDVYKHALSSTIRGDERENLLRRLGQETFFACEIEEKAARLGKLNMIVHAVNAQNAQWLHQNYLYN
EERGGLKPLIEYEVDFGEGKKKRQIGSSSIDLILTNPPFGKSVKTENVLLDYQFGHEVKTFKSAGRPPEKRAKNSQDSEV
LFIEHYLRTLKPGGKLLIVLPDGVLSNATAKPVRDYIREHAIIKSVISLPSETFASTGTSIPTNVVFLQKKRPGDVQGDI
FMARADYVGRRANGDPLKENDLPFILEKYREWQTGTLELQKEDSE

Sequences:

>Translated_605_residues
MATLEQFRDFLVTDLGFKAHQINDFNGAGVATTVDGLDEKAVFLVSTSVGNCYAAFRHEGSATLSESEANRYKALSILMP
NGAAAYCVIEVAGHLRIYLVEKGNEIHDEMNDWDNFRDAEDMVREFKPDVVNSLQQWARKTLHTESSLSRFVQLMKGCWQ
DIWDIENKRNDWIFDEFTRFLFIKLNEDAKPSGSFTTAKLTSYSEQNKHMGDKAAQNFINNLFDDLKAHHPEVFTDENER
VLSKAATVERVIARLEGINLKDTQGDVLGRAFEIMLSDTFKGKDLGQFFTPREIVAFMLDLARENPEGPALDISKGERFL
DGCAGSGGFLIAAYEDVYKHALSSTIRGDERENLLRRLGQETFFACEIEEKAARLGKLNMIVHAVNAQNAQWLHQNYLYN
EERGGLKPLIEYEVDFGEGKKKRQIGSSSIDLILTNPPFGKSVKTENVLLDYQFGHEVKTFKSAGRPPEKRAKNSQDSEV
LFIEHYLRTLKPGGKLLIVLPDGVLSNATAKPVRDYIREHAIIKSVISLPSETFASTGTSIPTNVVFLQKKRPGDVQGDI
FMARADYVGRRANGDPLKENDLPFILEKYREWQTGTLELQKEDSE
>Mature_604_residues
ATLEQFRDFLVTDLGFKAHQINDFNGAGVATTVDGLDEKAVFLVSTSVGNCYAAFRHEGSATLSESEANRYKALSILMPN
GAAAYCVIEVAGHLRIYLVEKGNEIHDEMNDWDNFRDAEDMVREFKPDVVNSLQQWARKTLHTESSLSRFVQLMKGCWQD
IWDIENKRNDWIFDEFTRFLFIKLNEDAKPSGSFTTAKLTSYSEQNKHMGDKAAQNFINNLFDDLKAHHPEVFTDENERV
LSKAATVERVIARLEGINLKDTQGDVLGRAFEIMLSDTFKGKDLGQFFTPREIVAFMLDLARENPEGPALDISKGERFLD
GCAGSGGFLIAAYEDVYKHALSSTIRGDERENLLRRLGQETFFACEIEEKAARLGKLNMIVHAVNAQNAQWLHQNYLYNE
ERGGLKPLIEYEVDFGEGKKKRQIGSSSIDLILTNPPFGKSVKTENVLLDYQFGHEVKTFKSAGRPPEKRAKNSQDSEVL
FIEHYLRTLKPGGKLLIVLPDGVLSNATAKPVRDYIREHAIIKSVISLPSETFASTGTSIPTNVVFLQKKRPGDVQGDIF
MARADYVGRRANGDPLKENDLPFILEKYREWQTGTLELQKEDSE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 68264; Mature: 68133

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLEQFRDFLVTDLGFKAHQINDFNGAGVATTVDGLDEKAVFLVSTSVGNCYAAFRHEG
CCCHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHCCC
SATLSESEANRYKALSILMPNGAAAYCVIEVAGHLRIYLVEKGNEIHDEMNDWDNFRDAE
CCCCCHHHCCCEEEEEEEECCCCCEEEEEEEECCEEEEEEECCCHHHHHHHHHCCCCCHH
DMVREFKPDVVNSLQQWARKTLHTESSLSRFVQLMKGCWQDIWDIENKRNDWIFDEFTRF
HHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCEE
LFIKLNEDAKPSGSFTTAKLTSYSEQNKHMGDKAAQNFINNLFDDLKAHHPEVFTDENER
EEEEECCCCCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VLSKAATVERVIARLEGINLKDTQGDVLGRAFEIMLSDTFKGKDLGQFFTPREIVAFMLD
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHH
LARENPEGPALDISKGERFLDGCAGSGGFLIAAYEDVYKHALSSTIRGDERENLLRRLGQ
HHHCCCCCCEEECCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHCC
ETFFACEIEEKAARLGKLNMIVHAVNAQNAQWLHQNYLYNEERGGLKPLIEYEVDFGEGK
CCEEEEEEHHHHHHHCHHEEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
KKRQIGSSSIDLILTNPPFGKSVKTENVLLDYQFGHEVKTFKSAGRPPEKRAKNSQDSEV
HHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCHHHHCCCCCCCCE
LFIEHYLRTLKPGGKLLIVLPDGVLSNATAKPVRDYIREHAIIKSVISLPSETFASTGTS
EEHHHHHHHCCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
IPTNVVFLQKKRPGDVQGDIFMARADYVGRRANGDPLKENDLPFILEKYREWQTGTLELQ
CCCEEEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEE
KEDSE
CCCCC
>Mature Secondary Structure 
ATLEQFRDFLVTDLGFKAHQINDFNGAGVATTVDGLDEKAVFLVSTSVGNCYAAFRHEG
CCHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHCCC
SATLSESEANRYKALSILMPNGAAAYCVIEVAGHLRIYLVEKGNEIHDEMNDWDNFRDAE
CCCCCHHHCCCEEEEEEEECCCCCEEEEEEEECCEEEEEEECCCHHHHHHHHHCCCCCHH
DMVREFKPDVVNSLQQWARKTLHTESSLSRFVQLMKGCWQDIWDIENKRNDWIFDEFTRF
HHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCEE
LFIKLNEDAKPSGSFTTAKLTSYSEQNKHMGDKAAQNFINNLFDDLKAHHPEVFTDENER
EEEEECCCCCCCCCEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
VLSKAATVERVIARLEGINLKDTQGDVLGRAFEIMLSDTFKGKDLGQFFTPREIVAFMLD
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHHH
LARENPEGPALDISKGERFLDGCAGSGGFLIAAYEDVYKHALSSTIRGDERENLLRRLGQ
HHHCCCCCCEEECCCCCHHHHCCCCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHCC
ETFFACEIEEKAARLGKLNMIVHAVNAQNAQWLHQNYLYNEERGGLKPLIEYEVDFGEGK
CCEEEEEEHHHHHHHCHHEEEEEECCCCCHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
KKRQIGSSSIDLILTNPPFGKSVKTENVLLDYQFGHEVKTFKSAGRPPEKRAKNSQDSEV
HHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHCCCCHHHHCCCCCCCCE
LFIEHYLRTLKPGGKLLIVLPDGVLSNATAKPVRDYIREHAIIKSVISLPSETFASTGTS
EEHHHHHHHCCCCCEEEEEECCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
IPTNVVFLQKKRPGDVQGDIFMARADYVGRRANGDPLKENDLPFILEKYREWQTGTLELQ
CCCEEEEEECCCCCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEE
KEDSE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]