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The map label for this gene is rep [H]

Identifier: 78049939

GI number: 78049939

Start: 5045650

End: 5047626

Strand: Reverse

Name: rep [H]

Synonym: XCV4383

Alternate gene names: 78049939

Gene position: 5047626-5045650 (Counterclockwise)

Preceding gene: 78049943

Following gene: 78049938

Centisome position: 97.47

GC content: 63.43

Gene sequence:

>1977_bases
ATGTACGGTCTCAATCCCCCGCAAAGCGCCGCCGTGCTGCACTGCGAAGGTCCCCTGTTGGTGCTGGCCGGCGCCGGCAG
CGGCAAGACGCGCGTGATCGTGGAAAAGATCGCGCATCTGATCGCCATCGGCCGCTATCCGGCCAAGCGGATCGCCGCGA
TCACCTTCACCAACAAATCCGCCAAGGAAATGCGCGAGCGTGTGGCCAAACGCATTCGCGGCGACGGTGCCGACGGCCTG
ACGATCTGCACCTTCCACGCACTGGGCCTGAAGTTTCTGCAGATCGAGCACGCCGCCGCCGGTTTGAAGCGCGGCTTTTC
GATCTTCGATTCGGACGATGCCGCCGCGCAGATCAAGGACCTGATGCATGGTGCCAAGCCCGATGCGATCGAAGATGCCA
AGAACCTGATCTCGCGCGCCAAGAATGCCGGCCTGTCGCCGGAGCAGGCGATGGCGGCCGCACGCAGCACCCGCGAAAAG
GAAGCGGCCGGCTTGTACGAGCGCTACCAGGCGCGGCTGACCACCTTCAACGCAGTGGACTTCGACGACCTGATCCGTCT
GCCGGTGCAGATCCTGGAAGCCAACGAAGACATCGTGATGGGCTGGCGCGAGCGCATCGGCTACCTGCTGGTGGACGAGT
GCCAGGACACCAACGATGCGCAGTACCGGCTGCTGAAGATGCTGGCCGGGCCGCGCGGCAACTTCACCTGCGTGGGCGAC
GACGACCAGAGCATCTATGCCTGGCGCGGCGCCAATCCGGAAAACCTGCAGCAGATGGCGCGCGATTATCCGGCGCTGGA
AATCATCAAGCTGGAACAGAACTACCGCTGCTCCAATCGTGTGCTGCGTGCGGCCAACGCGTTGATCGCGCACAACCCGC
ATGAGCATTTGAAGACCTTGTGGAGCGACCAGGCCGACGGCGAGCGCATCCGCGTGTGGGAATGCCGCGACAGCGAGCAC
GAAGCCGAAAAGGTCGCCGCGGAGATCTCGTTCCTGGGTACCGCCAAGCAGGTGCCGTGGAGCGACTTCTGCATTCTGTT
CCGTGGCAATTTTCAATCGCGGCCGCTGGAAAAAGCGCTGCAGCTATTGCGCGTGCCGTATCACCTCACCGGTGGTACCG
CGTTTCTGGAGCGGCAGGAGGTCAAGGACTTGCTGTCGTGGTTGCGGTTGATCGTCAACCCGGATGACGACGCGGCGTTC
TTGCGTGCCGTGCAGTCGCCCAAGCGCGAAGTCGGTGCGACCTCGCTGGCGCGTCTTGCCGAACTTGCCAGCGCCAAGTC
GGTACCGATGTCGCGCGCAGCCGAGTCGATGGGCGCCTTGCAGCATTTACCGCCGCGCGCAGCCAACGGTTTGAGTGCGT
TTACCGACATCCTGCGCGACATGCGCGAGCACTCGTCTAGCATGCCGGCCGGCGAGCTGGTGCGCCTGCTGGCCGACAAG
TCCGGTTTGCTCAACGACCTGCGCAATCAGTCGAAAGACGAGACCGGCTTCCAGCGGCGCAAGCGCAATCTGGACGAGCT
GGCCGAGTGGTTCGAGGGTGGTCCGCGCGGTGCAAGCGCCAGCGATCTGGCTGCGCAGTTGGCGTTGCTGTCGCGCAACG
ACAAGGACGATGGCGGCAACCAGGTGCGCATGATGACCATGCATGCGTCCAAGGGACTGGAATTCCGCTATGTCTTCATC
GTCGGCTGCGAAGACGGCGTGCTACCGCATGAAGTGAGCCTGGAAGAAGGCAATCTGCAGGAAGAGCGCCGCCTGCTGTA
CGTGGGCATCACCCGCGCCAAGGAGCAGCTGTGGATGAGCCACAGCAAGCTGACGCGCAAGTTCGGTGAGCATGTGCGAC
TGAAGCCGAGCCGGTTTTTCGATGAGATTCCGGCCGAAGAACTGCAGCGCGATGGTGCCGACCCGGTGGCCGACGCCGAG
CGCAAGAAGGAACGCGCCAGTGCGGGATTGGCTGCGATCCAGGCATTGTTCGACTGA

Upstream 100 bases:

>100_bases
CCGGTTGTGGGCAGGGCTAGCCGCACTGGAGACATGAATCCATCGCGTCGGCCAGCCGCGGGCAGCCCCGGTACAATGGC
GCGCTCTGTCGGAAGCCTCT

Downstream 100 bases:

>100_bases
GTTCTCCTGAGCCGCGCGGGCAGCTTTGGGCCACATGAGGTGAGTCTGGCAGGCAAGGCGGCGCTGGGCGCCTGCCGGAG
GCACTGCGGGTGAGTGCGTG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMRERVAKRIRGDGADGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREK
EAAGLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD

Sequences:

>Translated_658_residues
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMRERVAKRIRGDGADGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREK
EAAGLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD
>Mature_658_residues
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKSAKEMRERVAKRIRGDGADGL
TICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKDLMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREK
EAAGLYERYQARLTTFNAVDFDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTLWSDQADGERIRVWECRDSEH
EAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKALQLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAF
LRAVQSPKREVGATSLARLAELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGNQVRMMTMHASKGLEFRYVFI
VGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMSHSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAE
RKKERASAGLAAIQALFD

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=44.6268656716418, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI2367296, Length=642, Percent_Identity=38.4735202492212, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787196, Length=342, Percent_Identity=30.4093567251462, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=732, Percent_Identity=25.8196721311475, Blast_Score=186, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 73199; Mature: 73199

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKS
CCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCH
AKEMRERVAKRIRGDGADGLTICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKD
HHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREKEAAGLYERYQARLTTFNAVD
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WSDQADGERIRVWECRDSEHEAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAFLRAVQSPKREVGATSLARLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
ELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTMHASKGLEFRYVFIVGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
HSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAERKKERASAGLAAIQALFD
HHHHHHHHHHCEECCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYGLNPPQSAAVLHCEGPLLVLAGAGSGKTRVIVEKIAHLIAIGRYPAKRIAAITFTNKS
CCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEECCCH
AKEMRERVAKRIRGDGADGLTICTFHALGLKFLQIEHAAAGLKRGFSIFDSDDAAAQIKD
HHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LMHGAKPDAIEDAKNLISRAKNAGLSPEQAMAAARSTREKEAAGLYERYQARLTTFNAVD
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
FDDLIRLPVQILEANEDIVMGWRERIGYLLVDECQDTNDAQYRLLKMLAGPRGNFTCVGD
HHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEEECC
DDQSIYAWRGANPENLQQMARDYPALEIIKLEQNYRCSNRVLRAANALIAHNPHEHLKTL
CCCCEEEECCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHH
WSDQADGERIRVWECRDSEHEAEKVAAEISFLGTAKQVPWSDFCILFRGNFQSRPLEKAL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHH
QLLRVPYHLTGGTAFLERQEVKDLLSWLRLIVNPDDDAAFLRAVQSPKREVGATSLARLA
HHHHCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHHH
ELASAKSVPMSRAAESMGALQHLPPRAANGLSAFTDILRDMREHSSSMPAGELVRLLADK
HHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
SGLLNDLRNQSKDETGFQRRKRNLDELAEWFEGGPRGASASDLAAQLALLSRNDKDDGGN
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
QVRMMTMHASKGLEFRYVFIVGCEDGVLPHEVSLEEGNLQEERRLLYVGITRAKEQLWMS
EEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHCEEEEEEHHHHHHHHHHH
HSKLTRKFGEHVRLKPSRFFDEIPAEELQRDGADPVADAERKKERASAGLAAIQALFD
HHHHHHHHHHCEECCHHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]