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The map label for this gene is xthA2 [H]

Identifier: 78049826

GI number: 78049826

Start: 4903500

End: 4904291

Strand: Reverse

Name: xthA2 [H]

Synonym: XCV4270

Alternate gene names: 78049826

Gene position: 4904291-4903500 (Counterclockwise)

Preceding gene: 78049828

Following gene: 78049825

Centisome position: 94.71

GC content: 65.4

Gene sequence:

>792_bases
ATGGTCCGCGCCATGAAGATTGCCTCCTGGAACGTCAACTCGCTCAATGTGCGCCTGCCGCACCTGCAACAGTGGCTCGC
CGCGTTTGCGCCGGATGTGGTGGGCATCCAGGAAACCAAGCTGGAAGACCACAAGTTTCCCGATGCAGCGCTGGCGGCAC
TGGGCTACCGCAGCGTGTTCTGCGGGCAGAAAACCTATAACGGCGTGGCGATCCTGTCGCGCTCGCCGGCAGTTGACGTG
CAGATGGGCATCCCCGGGCTCGACGATGTGCAGCAGCGCGTCATCGCCGCCACGGTGGATGGCGTGCGCATCATCAATCT
GTATGTGGTCAATGGCCAGGACGTGGGCACCGACAAGTACGCCTACAAGTTGCGCTGGCTCGCCGCAGCGCACGACTGGA
TCGCGCAGGAGCTGCAGCGGTACCCGCAGCTGGTGGTGCTGGGCGACTTCAACATCGCCCCGGACGCGCGCGACGTGCAC
GATGCAACGGTGTGGAACGAGCACCACATCCTCACCTCGACCGACGAGCGCGCCGCGTTGGACAAACTGCTGGCGCTGGG
CTTGCACGACGCATTCCGGTTGCACAACCAGGACGCCGAGCACTTTAGTTGGTGGGATTACCGCCAGGCCGGTTTCCGCC
GCAATCTGGGCTTGCGTATCGACCTGACCCTGGTCTCGGACGCGTTGCGCACGCGCGCGGTGGAATCGGGCATCGACCGC
GAACCGCGGACCTGGGAGCGGCCCAGTGACCATGCGCCGGCGTGGGTGCGGCTGGCGGAGGTCGGCGCATGA

Upstream 100 bases:

>100_bases
CACGGCACGGCAGCGCGTGGCGGAATAGCCCGGTTCCGCCACGCCGGGCTCCACCGTCCTGAGGGCGACGCATGGCGCGG
TACCATTGGTGGTTTGAACC

Downstream 100 bases:

>100_bases
GCGAGCGGCATCTGCGTCGTAGCACGCACAAGGTGGGCGATGTCACGCTGGGGCCGGTGCATCGCCTGAGTTGCCACTGC
GGTGCAGTGCAAATCGATCT

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MVRAMKIASWNVNSLNVRLPHLQQWLAAFAPDVVGIQETKLEDHKFPDAALAALGYRSVFCGQKTYNGVAILSRSPAVDV
QMGIPGLDDVQQRVIAATVDGVRIINLYVVNGQDVGTDKYAYKLRWLAAAHDWIAQELQRYPQLVVLGDFNIAPDARDVH
DATVWNEHHILTSTDERAALDKLLALGLHDAFRLHNQDAEHFSWWDYRQAGFRRNLGLRIDLTLVSDALRTRAVESGIDR
EPRTWERPSDHAPAWVRLAEVGA

Sequences:

>Translated_263_residues
MVRAMKIASWNVNSLNVRLPHLQQWLAAFAPDVVGIQETKLEDHKFPDAALAALGYRSVFCGQKTYNGVAILSRSPAVDV
QMGIPGLDDVQQRVIAATVDGVRIINLYVVNGQDVGTDKYAYKLRWLAAAHDWIAQELQRYPQLVVLGDFNIAPDARDVH
DATVWNEHHILTSTDERAALDKLLALGLHDAFRLHNQDAEHFSWWDYRQAGFRRNLGLRIDLTLVSDALRTRAVESGIDR
EPRTWERPSDHAPAWVRLAEVGA
>Mature_263_residues
MVRAMKIASWNVNSLNVRLPHLQQWLAAFAPDVVGIQETKLEDHKFPDAALAALGYRSVFCGQKTYNGVAILSRSPAVDV
QMGIPGLDDVQQRVIAATVDGVRIINLYVVNGQDVGTDKYAYKLRWLAAAHDWIAQELQRYPQLVVLGDFNIAPDARDVH
DATVWNEHHILTSTDERAALDKLLALGLHDAFRLHNQDAEHFSWWDYRQAGFRRNLGLRIDLTLVSDALRTRAVESGIDR
EPRTWERPSDHAPAWVRLAEVGA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=265, Percent_Identity=31.3207547169811, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI18375503, Length=265, Percent_Identity=31.3207547169811, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI18375501, Length=265, Percent_Identity=31.3207547169811, Blast_Score=98, Evalue=7e-21,
Organism=Escherichia coli, GI1788046, Length=263, Percent_Identity=38.4030418250951, Blast_Score=172, Evalue=2e-44,
Organism=Drosophila melanogaster, GI221330655, Length=255, Percent_Identity=30.1960784313725, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17136678, Length=255, Percent_Identity=30.1960784313725, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29617; Mature: 29617

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRAMKIASWNVNSLNVRLPHLQQWLAAFAPDVVGIQETKLEDHKFPDAALAALGYRSVF
CCEEEEEECCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CGQKTYNGVAILSRSPAVDVQMGIPGLDDVQQRVIAATVDGVRIINLYVVNGQDVGTDKY
CCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHH
AYKLRWLAAAHDWIAQELQRYPQLVVLGDFNIAPDARDVHDATVWNEHHILTSTDERAAL
HEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEECCCEEEECCCHHHHH
DKLLALGLHDAFRLHNQDAEHFSWWDYRQAGFRRNLGLRIDLTLVSDALRTRAVESGIDR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCC
EPRTWERPSDHAPAWVRLAEVGA
CCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure
MVRAMKIASWNVNSLNVRLPHLQQWLAAFAPDVVGIQETKLEDHKFPDAALAALGYRSVF
CCEEEEEECCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CGQKTYNGVAILSRSPAVDVQMGIPGLDDVQQRVIAATVDGVRIINLYVVNGQDVGTDKY
CCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHH
AYKLRWLAAAHDWIAQELQRYPQLVVLGDFNIAPDARDVHDATVWNEHHILTSTDERAAL
HEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEECCCEEEECCCHHHHH
DKLLALGLHDAFRLHNQDAEHFSWWDYRQAGFRRNLGLRIDLTLVSDALRTRAVESGIDR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCCCC
EPRTWERPSDHAPAWVRLAEVGA
CCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]