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The map label for this gene is hemB [H]

Identifier: 78049687

GI number: 78049687

Start: 4727541

End: 4728533

Strand: Reverse

Name: hemB [H]

Synonym: XCV4131

Alternate gene names: 78049687

Gene position: 4728533-4727541 (Counterclockwise)

Preceding gene: 78049688

Following gene: 78049685

Centisome position: 91.31

GC content: 64.65

Gene sequence:

>993_bases
ATGGCCCACCCGCACTATCGCCCCCGCCGCATGCGCCACGATGGGTTTTCGCGTCGGTTGATGCGCGAGAACACGCTGAC
CACCGATGACCTGATCTGGCCGGTGTTCGTACACGAACTGTCCGGCCGCGCGCCGATCGCGTCGATGCCCGGCGTGGAAC
GCCTGTCGCTGGACGAGCTGCTGCGCGAGGCGGAAAACGCGCTGGAACTGGGCATCCCGGTGATCGACCTGTTCCCGGTG
ATCGACCCGGCCGGCAAGAGCCTGGACGCGGCCGAAGCCTGGAACCCGGACGGCCTGGCGCAGCGCGCGGTGCGGGCGTT
GAAGTCGCGCTTCCCTGAGCTGGGCGTCATGACCGACGTGGCGCTGGACCCGTACACCACCCACGGCCAGGACGGCATCA
TCGACGACAAGGGTTATGTGCTCAACGAGATCACGGTCGAGGCTTTGGTCAAGCAATCGTTGTCGCATGCGCAGGCGGGC
GTGGACATCATTTCGCCTTCGGACATGATGGATGGCCGCATCGGTGCGATCCGCCGCGCGCTCGATGCCGACGATCATCT
CAACGTGCGGATCATGGCGTATTCGGCCAAGTACGCCTCGGCGTTCTACGGTCCCTTCCGCGATGCGGTAGGCAGCGCCG
GCAATCTGGGCAAGGCCGACAAGACCACCTACCAGATGGACCCGGCCAACGGCGATGAAGCCTTGCGCGAGATCGCACTG
GATTTGGAAGAAGGCGCGGACATGGTGATGGTCAAGCCGGGCATGCCGTATCTGGATGTAGTGCGCCGGGTGAAGGATCA
ATTCCGCGTGCCGACCTTTGCGTATCAGGTCAGCGGCGAGTACGCGATGCTCAAGGCGGCGTCAGCCAACGGCTGGCTGG
ACGAGCGCAAGTGCGTGCTCGAAGCATTGATGGCCTTCAAGCGTGCCGGCGCCGATGGCGTGTTGACCTATTTCGCGCCG
CAAGTGGCGCGCTGGTTGCGCGAAACACGCTGA

Upstream 100 bases:

>100_bases
AAGAACCCGCGTGCCGTTGCAGCGCTGCGACACTGCGTCGCAGCGCGTTCTGCGCATCATGGCGGGCGTAAACTGATGCG
CTGTCTACAGGAGCCTTGTC

Downstream 100 bases:

>100_bases
GCGCGAGCACGGTGCGACGACGCTGCCACTCAGTTGCGCAAGAATGAGGGCCGCTCGCGTCGGCCAAGCAGTCGCCTGCG
CGCAGTAGGATCCGGAGCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAHPHYRPRRMRHDGFSRRLMRENTLTTDDLIWPVFVHELSGRAPIASMPGVERLSLDELLREAENALELGIPVIDLFPV
IDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDVALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAQAG
VDIISPSDMMDGRIGAIRRALDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
DLEEGADMVMVKPGMPYLDVVRRVKDQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVLEALMAFKRAGADGVLTYFAP
QVARWLRETR

Sequences:

>Translated_330_residues
MAHPHYRPRRMRHDGFSRRLMRENTLTTDDLIWPVFVHELSGRAPIASMPGVERLSLDELLREAENALELGIPVIDLFPV
IDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDVALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAQAG
VDIISPSDMMDGRIGAIRRALDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
DLEEGADMVMVKPGMPYLDVVRRVKDQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVLEALMAFKRAGADGVLTYFAP
QVARWLRETR
>Mature_329_residues
AHPHYRPRRMRHDGFSRRLMRENTLTTDDLIWPVFVHELSGRAPIASMPGVERLSLDELLREAENALELGIPVIDLFPVI
DPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDVALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAQAGV
DIISPSDMMDGRIGAIRRALDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIALD
LEEGADMVMVKPGMPYLDVVRRVKDQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVLEALMAFKRAGADGVLTYFAPQ
VARWLRETR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=330, Percent_Identity=41.8181818181818, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=44.9685534591195, Blast_Score=253, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=327, Percent_Identity=36.085626911315, Blast_Score=193, Evalue=3e-50,
Organism=Drosophila melanogaster, GI21358291, Length=318, Percent_Identity=39.622641509434, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36549; Mature: 36418

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPHYRPRRMRHDGFSRRLMRENTLTTDDLIWPVFVHELSGRAPIASMPGVERLSLDEL
CCCCCCCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
LREAENALELGIPVIDLFPVIDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDV
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHC
ALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAQAGVDIISPSDMMDGRIGAIRRA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCHHHHHHHH
LDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
HCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
DLEEGADMVMVKPGMPYLDVVRRVKDQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVL
HHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCHHHHHHHH
EALMAFKRAGADGVLTYFAPQVARWLRETR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AHPHYRPRRMRHDGFSRRLMRENTLTTDDLIWPVFVHELSGRAPIASMPGVERLSLDEL
CCCCCCCHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHH
LREAENALELGIPVIDLFPVIDPAGKSLDAAEAWNPDGLAQRAVRALKSRFPELGVMTDV
HHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHC
ALDPYTTHGQDGIIDDKGYVLNEITVEALVKQSLSHAQAGVDIISPSDMMDGRIGAIRRA
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHCCHHHHHHHH
LDADDHLNVRIMAYSAKYASAFYGPFRDAVGSAGNLGKADKTTYQMDPANGDEALREIAL
HCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHH
DLEEGADMVMVKPGMPYLDVVRRVKDQFRVPTFAYQVSGEYAMLKAASANGWLDERKCVL
HHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCHHHHHHHH
EALMAFKRAGADGVLTYFAPQVARWLRETR
HHHHHHHHCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]