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The map label for this gene is dusA [H]

Identifier: 78049674

GI number: 78049674

Start: 4713413

End: 4714417

Strand: Reverse

Name: dusA [H]

Synonym: XCV4118

Alternate gene names: 78049674

Gene position: 4714417-4713413 (Counterclockwise)

Preceding gene: 78049675

Following gene: 78049673

Centisome position: 91.04

GC content: 68.36

Gene sequence:

>1005_bases
ATGACCGCTTCCGCCGATCGCTACGCCGCCTCCCTGCGCCTGTCCGTCGCTCCGATGATGGATTGGACCGACCGCCATTG
CCGGGTCTTCCACCGCCTGTTGGCGCCGTCCGCGCGCCTGTACACCGAGATGGTGCACGCCAACGCGGTGATCCACGGCG
ATCGCGCCCGCTTGATCGGCTTCGATGCGGTGGAACATCCGCTGGCGCTGCAGCTGGGCGGCAGCGATCCCGCCTTGCTC
GCGCAGGCGGCGGCGATTGCGCAGGAGTGGGGCTTTGATGAGATCAATCTCAACTGCGGTTGCCCCTCCGACCGTGTGCA
GGCCGGGCGTTTCGGCGCCTGCCTGATGCGCGAGCCGGCGCTGGTGGCCGAGTGCGTCGCGGCCATGTGCGCGGCCACCC
ACGTGCCGATCACCGTCAAGTGCCGCCTGGGCGTGGACGACGATGACGACTACGCGCTGTTTGCCCGCTTTGTCGACCAG
GTGGTCGCGGCCGGCGCGGCCATGGTGGTGGTGCATGCCCGGAACGCCTGGCTCAAGGGCTTGTCGCCGAAGGAAAACCG
CGAAGTGCCGCCGCTGCGGTACGACTGGGCTTACCGGCTCAAGCAGGAGCGGCCCGGTCTGCCGGTCGTGCTCAACGGCG
GCATCGTGGCCGTGGACACGGCGATGGCGCATCTGCAGCACGCCGACGGTGTCATGCTCGGCCGCGCCGCCTACCATGAC
CCGTACGTCCTGCATTGTCTGGATGCAGCCTTGAACCAGCGGCCCGAGCAATCGCGCGAGTCCTTGCTGCGCGCCTATCA
GCCGTACGTGGAATCCCAACTGGCACAGGGTCTGGCCTTGAAACACATGACCCGGCACGTCCTCGGTCTGTTTCATGGTC
AGCCTGGAGGCAGGGCGTTCCGGCAGGTCCTGAGCGAGGGCGCCCATCGCCCGGGCGCCGGTTGGGAACTGGTCGAGCAG
GCCCTGGAGCGCACCGATACGCGGTCCTGGCGCGTGGTTGCCTGA

Upstream 100 bases:

>100_bases
CCAGCCGCCCACTCCGCACCGCCGCAGATTCATCGACGCCCGTGTAAGCTGTTGATCCTGCTTCGCATTCTAATGGCTGC
TCGCTGCGGCCACTTCGTCG

Downstream 100 bases:

>100_bases
CTTTGGCCCACCGCTTGCATTCCTGAACGGGCTGCCTGGATCTCCGAGCGTTTCGGGTGGCTGTTGCAGCAGGCGCGTCC
GCAACACCACGTGCTCGCCG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIGFDAVEHPLALQLGGSDPALL
AQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCAATHVPITVKCRLGVDDDDDYALFARFVDQ
VVAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVDTAMAHLQHADGVMLGRAAYHD
PYVLHCLDAALNQRPEQSRESLLRAYQPYVESQLAQGLALKHMTRHVLGLFHGQPGGRAFRQVLSEGAHRPGAGWELVEQ
ALERTDTRSWRVVA

Sequences:

>Translated_334_residues
MTASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIGFDAVEHPLALQLGGSDPALL
AQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCAATHVPITVKCRLGVDDDDDYALFARFVDQ
VVAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVDTAMAHLQHADGVMLGRAAYHD
PYVLHCLDAALNQRPEQSRESLLRAYQPYVESQLAQGLALKHMTRHVLGLFHGQPGGRAFRQVLSEGAHRPGAGWELVEQ
ALERTDTRSWRVVA
>Mature_333_residues
TASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIGFDAVEHPLALQLGGSDPALLA
QAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPALVAECVAAMCAATHVPITVKCRLGVDDDDDYALFARFVDQV
VAAGAAMVVVHARNAWLKGLSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVDTAMAHLQHADGVMLGRAAYHDP
YVLHCLDAALNQRPEQSRESLLRAYQPYVESQLAQGLALKHMTRHVLGLFHGQPGGRAFRQVLSEGAHRPGAGWELVEQA
LERTDTRSWRVVA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=233, Percent_Identity=28.755364806867, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI40807366, Length=242, Percent_Identity=26.8595041322314, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=52.8481012658228, Blast_Score=340, Evalue=7e-95,
Organism=Escherichia coli, GI1788462, Length=214, Percent_Identity=32.7102803738318, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789660, Length=257, Percent_Identity=28.7937743190661, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI25144369, Length=256, Percent_Identity=25.390625, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=167, Percent_Identity=28.1437125748503, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=284, Percent_Identity=23.943661971831, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=28.5123966942149, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=28.5123966942149, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585320, Length=187, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36674; Mature: 36543

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIG
CCCCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEECCCEEEEE
FDAVEHPLALQLGGSDPALLAQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPA
CHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHCCHH
LVAECVAAMCAATHVPITVKCRLGVDDDDDYALFARFVDQVVAAGAAMVVVHARNAWLKG
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCC
LSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVDTAMAHLQHADGVMLGRAAYHD
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHCCCEEECHHHCCC
PYVLHCLDAALNQRPEQSRESLLRAYQPYVESQLAQGLALKHMTRHVLGLFHGQPGGRAF
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RQVLSEGAHRPGAGWELVEQALERTDTRSWRVVA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure 
TASADRYAASLRLSVAPMMDWTDRHCRVFHRLLAPSARLYTEMVHANAVIHGDRARLIG
CCCCHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCEEEECCCEEEEE
FDAVEHPLALQLGGSDPALLAQAAAIAQEWGFDEINLNCGCPSDRVQAGRFGACLMREPA
CHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCCCCCHHHHCCHH
LVAECVAAMCAATHVPITVKCRLGVDDDDDYALFARFVDQVVAAGAAMVVVHARNAWLKG
HHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEECHHHHCC
LSPKENREVPPLRYDWAYRLKQERPGLPVVLNGGIVAVDTAMAHLQHADGVMLGRAAYHD
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEECCCEEEEHHHHHHHHHCCCEEECHHHCCC
PYVLHCLDAALNQRPEQSRESLLRAYQPYVESQLAQGLALKHMTRHVLGLFHGQPGGRAF
CHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
RQVLSEGAHRPGAGWELVEQALERTDTRSWRVVA
HHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]