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The map label for this gene is glnB2 [H]

Identifier: 78049601

GI number: 78049601

Start: 4646759

End: 4647097

Strand: Reverse

Name: glnB2 [H]

Synonym: XCV4045

Alternate gene names: 78049601

Gene position: 4647097-4646759 (Counterclockwise)

Preceding gene: 78049605

Following gene: 78049600

Centisome position: 89.74

GC content: 63.72

Gene sequence:

>339_bases
ATGAAAATGATCATGGCCATCGTCAAACCGTTCAAGCTCGACGATGTGCGCGAAGCGCTCGCCGGCTGCGGGGTGGCGGG
CATCACGGTGACCGAGGTCAAAGGCTTCGGCCGGCAGAAAGGCCATACCGAGCTCTATCGCGGCGCCGAATACGTGGTCG
ATTTCCTGCCCAAGGTGAAGATCGAAGTGGCGGTGACCGACGACCAGGCCGAGCGTGTGGTCGAGGCGATCGTCGCGGCG
GCCGGTACCGGCAAGATCGGCGACGGCAAGGTGTTCGTGTACGACCTGGGCACGGTGGTACGCATCCGTACCGGCGAGCT
GGATAGCGACGCGCTGTAG

Upstream 100 bases:

>100_bases
CATTCGGCTGTCGTCCGCGGTGATGGCGGCGCGCGGTTGGATGAGTGTTGCGCCCGTCCACGGTATGCTGCGCGGCCGTC
ATGCCCTGGGACCTGCACCG

Downstream 100 bases:

>100_bases
GTTCGCAACGCGGCGCCCGCTGCTTGCCGGATGTGCGGTGGGTGGTGAGGGGTTTGCTGCCCTCTGCCGGCGCAACGACG
ATGCCCGGGCCACGTATTGC

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKMIMAIVKPFKLDDVREALAGCGVAGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEVAVTDDQAERVVEAIVAA
AGTGKIGDGKVFVYDLGTVVRIRTGELDSDAL

Sequences:

>Translated_112_residues
MKMIMAIVKPFKLDDVREALAGCGVAGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEVAVTDDQAERVVEAIVAA
AGTGKIGDGKVFVYDLGTVVRIRTGELDSDAL
>Mature_112_residues
MKMIMAIVKPFKLDDVREALAGCGVAGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIEVAVTDDQAERVVEAIVAA
AGTGKIGDGKVFVYDLGTVVRIRTGELDSDAL

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=70.5357142857143, Blast_Score=159, Evalue=4e-41,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=66.9642857142857, Blast_Score=157, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 11999; Mature: 11999

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMIMAIVKPFKLDDVREALAGCGVAGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
IEVAVTDDQAERVVEAIVAAAGTGKIGDGKVFVYDLGTVVRIRTGELDSDAL
EEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECCCCCCCC
>Mature Secondary Structure
MKMIMAIVKPFKLDDVREALAGCGVAGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CCHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
IEVAVTDDQAERVVEAIVAAAGTGKIGDGKVFVYDLGTVVRIRTGELDSDAL
EEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]