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The map label for this gene is rsmB [H]

Identifier: 78049480

GI number: 78049480

Start: 4504243

End: 4505553

Strand: Reverse

Name: rsmB [H]

Synonym: XCV3924

Alternate gene names: 78049480

Gene position: 4505553-4504243 (Counterclockwise)

Preceding gene: 78049481

Following gene: 78049479

Centisome position: 87.01

GC content: 69.34

Gene sequence:

>1311_bases
ATGTCGGCCGATACCGCAACCGCCGGCGTTGCCTCGCGCCTGGCCGCCGCACGCGTGCTGACGGCGGTGTTCGATCAGGG
CCGGTCGTTGAAGGCCGAACTTGCCGCCACCTTGCCCGGCATCGGCGATCCGCGCGATCGTGCGCTGGTGGAGGCGATCT
GCTTTGCGGTCATTCGCCGGCGTCCGGCCTATGACGTCGCGTTGCGGCAATGGCTGGAACGTGCGTTGCCGCCCCGCGAT
GCCGAACTCAAGGCGCTGCTGATGGTCGGTTTCGCCCAACTCGACGTGCTGCAGCTGCCCGCGCACGCCGCGTTGTCGGC
CACGGTGGATGCGTGCCGCGCATTGGGCCGGCCCCGTCAGGCCGGCATGGTCAACGCGATCTTGCGGCGCGCCCAGCGCG
AAGGATTTCCGGCGGTGGCCGACGATGCCGGTTGGCCGTCGTGGCTGCGCAAGCAGCTGCGCGCGGACTGGGGCGATCGT
GCCGAAGCCATTTTTACCGCCAGTGCGCAGATGGCGCCGATGTGGCTGCGCGTGCAGCGTTCGCGCACCGACCCTGCGAG
GTATGTCGCGCGACTGGCCGAACTGGATATCGTCGCGCACACCGACGCGGTACTGCCGGATGCGGTGCGCCTGCACAACG
CCGTGCCGGTGAGCCAGTTGCCGGGCTTTGCCGAAGGCGATGTTTCGGTGCAGGACGGCTCTGCCCAGCAGGTGGCCGAT
GCGTTGACCCTGGCGCCGTCGGCACGCGTGCTCGATGCCTGCGCGGCGCCGGGCGGCAAGGCGGCGCATCTGCTGGAGCG
CCACCCCGGCTTGCAGCTCACTGCGCTGGATGTGGATGCGCGCCGCCTGGAGCGCGTGCAGCAGACCTTGCAGCGCACAG
TCCCCGATGCGCAGGTTGCATTGCAGGCGGCCGACGCCGCCGATCCGGACGCGTGGTGGGACGGCCGGCCGTTCGATGCG
GTGCTGCTGGACGCGCCATGTTCGGCCACCGGCGTGGTGCGTCGTCAACCGGATGTGCTGCTGCACCGGCGCGCCGACGA
TATCGATGCGCTGTGCGCGCTGCAGGCGCGGCTACTGGATGCGACCTGGCGCACGCTGCGCCCAGGTGGACAATTGCTGT
ACACCACCTGTTCGCTGTTGGCGCGCGAGAACCAGGCGCAGGTCGAGGCATTCCTGCAGCGCACTCCCGATGCACAGGCG
CAGCCTTTGGGTGCGCAGTTCGGGCATGCCGCCGGCGCCGGACGTCAGCGGTTTCCAGGCGAGGCGCACTGCGACGGATT
TTTCTACGCGCTTTTGCTAAAAGCCTCCTGA

Upstream 100 bases:

>100_bases
AGGGTGCGCTGCGGGTGCGCGTGCTCCAGCGCGAGGGCGGCAAGGCGATCACCGCCGCCGATTACCTCAACGCGCGACGC
GACCTGCCGGCGCTGCGCTG

Downstream 100 bases:

>100_bases
TCGCGCGAAGTCCAGCGCTCCACCCTCTGCGTCCGACTGCCGCCCGAGATGTTCAAGAATCGCGCTTCCACCGATGTCTG
GTTGCTGGCCATGACCGCCG

Product: ribosomal RNA small subunit methyltransferase B

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSADTATAGVASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRRRPAYDVALRQWLERALPPRD
AELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQAGMVNAILRRAQREGFPAVADDAGWPSWLRKQLRADWGDR
AEAIFTASAQMAPMWLRVQRSRTDPARYVARLAELDIVAHTDAVLPDAVRLHNAVPVSQLPGFAEGDVSVQDGSAQQVAD
ALTLAPSARVLDACAAPGGKAAHLLERHPGLQLTALDVDARRLERVQQTLQRTVPDAQVALQAADAADPDAWWDGRPFDA
VLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLDATWRTLRPGGQLLYTTCSLLARENQAQVEAFLQRTPDAQA
QPLGAQFGHAAGAGRQRFPGEAHCDGFFYALLLKAS

Sequences:

>Translated_436_residues
MSADTATAGVASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRRRPAYDVALRQWLERALPPRD
AELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQAGMVNAILRRAQREGFPAVADDAGWPSWLRKQLRADWGDR
AEAIFTASAQMAPMWLRVQRSRTDPARYVARLAELDIVAHTDAVLPDAVRLHNAVPVSQLPGFAEGDVSVQDGSAQQVAD
ALTLAPSARVLDACAAPGGKAAHLLERHPGLQLTALDVDARRLERVQQTLQRTVPDAQVALQAADAADPDAWWDGRPFDA
VLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLDATWRTLRPGGQLLYTTCSLLARENQAQVEAFLQRTPDAQA
QPLGAQFGHAAGAGRQRFPGEAHCDGFFYALLLKAS
>Mature_435_residues
SADTATAGVASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRRRPAYDVALRQWLERALPPRDA
ELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQAGMVNAILRRAQREGFPAVADDAGWPSWLRKQLRADWGDRA
EAIFTASAQMAPMWLRVQRSRTDPARYVARLAELDIVAHTDAVLPDAVRLHNAVPVSQLPGFAEGDVSVQDGSAQQVADA
LTLAPSARVLDACAAPGGKAAHLLERHPGLQLTALDVDARRLERVQQTLQRTVPDAQVALQAADAADPDAWWDGRPFDAV
LLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLDATWRTLRPGGQLLYTTCSLLARENQAQVEAFLQRTPDAQAQ
PLGAQFGHAAGAGRQRFPGEAHCDGFFYALLLKAS

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=254, Percent_Identity=31.1023622047244, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI76150623, Length=254, Percent_Identity=31.1023622047244, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI32698918, Length=190, Percent_Identity=31.0526315789474, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI40316918, Length=162, Percent_Identity=31.4814814814815, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI11545785, Length=164, Percent_Identity=34.7560975609756, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI23199998, Length=258, Percent_Identity=30.6201550387597, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8922322, Length=258, Percent_Identity=30.6201550387597, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288816, Length=258, Percent_Identity=30.6201550387597, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI270288818, Length=258, Percent_Identity=30.6201550387597, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI301336155, Length=187, Percent_Identity=28.8770053475936, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI39995082, Length=187, Percent_Identity=28.8770053475936, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367212, Length=428, Percent_Identity=40.4205607476636, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI87081985, Length=237, Percent_Identity=33.7552742616034, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17536757, Length=243, Percent_Identity=30.8641975308642, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25143471, Length=203, Percent_Identity=32.0197044334975, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=239, Percent_Identity=27.1966527196653, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319447, Length=188, Percent_Identity=31.3829787234043, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI22024126, Length=250, Percent_Identity=30, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920494, Length=174, Percent_Identity=31.6091954022989, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21355201, Length=203, Percent_Identity=33.4975369458128, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24668781, Length=249, Percent_Identity=30.1204819277108, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21356513, Length=249, Percent_Identity=30.1204819277108, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46973; Mature: 46842

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADTATAGVASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
RPAYDVALRQWLERALPPRDAELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQ
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
AGMVNAILRRAQREGFPAVADDAGWPSWLRKQLRADWGDRAEAIFTASAQMAPMWLRVQR
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEECCCHHCHHHHHHHH
SRTDPARYVARLAELDIVAHTDAVLPDAVRLHNAVPVSQLPGFAEGDVSVQDGSAQQVAD
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCEECCCCHHHHHH
ALTLAPSARVLDACAAPGGKAAHLLERHPGLQLTALDVDARRLERVQQTLQRTVPDAQVA
HHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHH
LQAADAADPDAWWDGRPFDAVLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLD
HHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
ATWRTLRPGGQLLYTTCSLLARENQAQVEAFLQRTPDAQAQPLGAQFGHAAGAGRQRFPG
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCC
EAHCDGFFYALLLKAS
CCCCCCEEEHHHEECC
>Mature Secondary Structure 
SADTATAGVASRLAAARVLTAVFDQGRSLKAELAATLPGIGDPRDRALVEAICFAVIRR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
RPAYDVALRQWLERALPPRDAELKALLMVGFAQLDVLQLPAHAALSATVDACRALGRPRQ
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
AGMVNAILRRAQREGFPAVADDAGWPSWLRKQLRADWGDRAEAIFTASAQMAPMWLRVQR
CHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEECCCHHCHHHHHHHH
SRTDPARYVARLAELDIVAHTDAVLPDAVRLHNAVPVSQLPGFAEGDVSVQDGSAQQVAD
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHCCCCCCCCCEECCCCHHHHHH
ALTLAPSARVLDACAAPGGKAAHLLERHPGLQLTALDVDARRLERVQQTLQRTVPDAQVA
HHHCCCCHHHHHHHCCCCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHH
LQAADAADPDAWWDGRPFDAVLLDAPCSATGVVRRQPDVLLHRRADDIDALCALQARLLD
HHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHH
ATWRTLRPGGQLLYTTCSLLARENQAQVEAFLQRTPDAQAQPLGAQFGHAAGAGRQRFPG
HHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCC
EAHCDGFFYALLLKAS
CCCCCCEEEHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]