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The map label for this gene is yafC [H]

Identifier: 78049440

GI number: 78049440

Start: 4471403

End: 4472329

Strand: Reverse

Name: yafC [H]

Synonym: XCV3884

Alternate gene names: 78049440

Gene position: 4472329-4471403 (Counterclockwise)

Preceding gene: 78049443

Following gene: 78049436

Centisome position: 86.36

GC content: 66.77

Gene sequence:

>927_bases
ATGGAAGACCTCAACGACTTCGCGCTGCTGGTGGCAGTGGTCGACCACGGCAGCTTCTCGGCGGCCGGTCGCGCGTTGGG
GTTTCCCCGCTCCAAGGTCAGTCGGCGGGTGGCAGATCTGGAGCAACGCCTGGGCGTGCGGCTGTTGCAGCGTTCAACCC
GTGCGGTCAGCCTCACCGAGGTCGGTGCCGAGTTCTATACGCACTGCAAATCCATCACCCGCTCGGCGCAATGGGCGCTG
GATGTGGCCGAGCGCGCCACGCAGCATCCCTCCGGCCGGATCCGCGTGAGCTGCCCGACCGGTGTGGCGCATCTGTTTCT
GGCCAAGCTCTTGCCGCGGTTTTTGTTGGCGCATCCGGATGTGCAGGTCGATCTGGAGCTGACCAGTCGACGGGTGGAAC
TGATTGCCGAAGGCATCGACGTGGCGATACGGGTGCGTTCGAGCCTGGACGATTCCGGCTTGATCCTGCGCTCGTTCGGC
GTCAGCCGGCAGGCGTTGGTGGCAAGCCCCGGGTTCGTGCACGCGCATGGGCCGTTCGCCTCTGCCGCGGCACTGCATGA
GGTACTGGGAATGGGCCCGCGTGGCGTCAATGGCGAGCCGCCCCGGTGGCGGCTCACCAGCCCGCAAGGCACCAGCGAAC
TGGTGGACTACCGTTCGCGCCTGTTCACCGAGGATGTCGAGCTGCTGCACCAAGCGGCGCTGGCCGGCATGGGCGTGGCG
CAGTTGCCGCTGGCCCTGTGCAGGGGCAGCATCGCCCGTGGCGAACTGGTGGTGCTATTGGGCGACCACACGTTGCCGGT
GCATCAACTACACGCCGTGTATCCCTCGCGTCGCGGACTGGCGCCAGGCGTGCGCGCCTTGATCGAGTTCCTGGTCGACG
TCCTGCCTGCGGTGCTGGAGAGCGCCGAACACGAGGTATCAGGCTAA

Upstream 100 bases:

>100_bases
CTCTACGAAGCGCCCTCGGGCGCAGGTAGGCCGCTGGCGGATGACAAACTGTCCTGCCTGGCAGGACAATGACGGCCTCC
TTGCGTGGGGTTGGATTCAG

Downstream 100 bases:

>100_bases
GCGCAGCGAACAAAACGACTGCGCAGCGCCAGGCGGGCGCGGCCGCTGCCGGGCATCGGCATATACCACTCGTACACGGC
GGTTCTGAGCGCGCCGTCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEDLNDFALLVAVVDHGSFSAAGRALGFPRSKVSRRVADLEQRLGVRLLQRSTRAVSLTEVGAEFYTHCKSITRSAQWAL
DVAERATQHPSGRIRVSCPTGVAHLFLAKLLPRFLLAHPDVQVDLELTSRRVELIAEGIDVAIRVRSSLDDSGLILRSFG
VSRQALVASPGFVHAHGPFASAAALHEVLGMGPRGVNGEPPRWRLTSPQGTSELVDYRSRLFTEDVELLHQAALAGMGVA
QLPLALCRGSIARGELVVLLGDHTLPVHQLHAVYPSRRGLAPGVRALIEFLVDVLPAVLESAEHEVSG

Sequences:

>Translated_308_residues
MEDLNDFALLVAVVDHGSFSAAGRALGFPRSKVSRRVADLEQRLGVRLLQRSTRAVSLTEVGAEFYTHCKSITRSAQWAL
DVAERATQHPSGRIRVSCPTGVAHLFLAKLLPRFLLAHPDVQVDLELTSRRVELIAEGIDVAIRVRSSLDDSGLILRSFG
VSRQALVASPGFVHAHGPFASAAALHEVLGMGPRGVNGEPPRWRLTSPQGTSELVDYRSRLFTEDVELLHQAALAGMGVA
QLPLALCRGSIARGELVVLLGDHTLPVHQLHAVYPSRRGLAPGVRALIEFLVDVLPAVLESAEHEVSG
>Mature_308_residues
MEDLNDFALLVAVVDHGSFSAAGRALGFPRSKVSRRVADLEQRLGVRLLQRSTRAVSLTEVGAEFYTHCKSITRSAQWAL
DVAERATQHPSGRIRVSCPTGVAHLFLAKLLPRFLLAHPDVQVDLELTSRRVELIAEGIDVAIRVRSSLDDSGLILRSFG
VSRQALVASPGFVHAHGPFASAAALHEVLGMGPRGVNGEPPRWRLTSPQGTSELVDYRSRLFTEDVELLHQAALAGMGVA
QLPLALCRGSIARGELVVLLGDHTLPVHQLHAVYPSRRGLAPGVRALIEFLVDVLPAVLESAEHEVSG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=35.1724137931034, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1789639, Length=303, Percent_Identity=32.6732673267327, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=29.042904290429, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=27.8350515463918, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081978, Length=288, Percent_Identity=29.5138888888889, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=28.8659793814433, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=27.1428571428571, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=25, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=176, Percent_Identity=27.8409090909091, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33257; Mature: 33257

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDLNDFALLVAVVDHGSFSAAGRALGFPRSKVSRRVADLEQRLGVRLLQRSTRAVSLTE
CCCHHHHHHHHHHHHCCCCHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHH
VGAEFYTHCKSITRSAQWALDVAERATQHPSGRIRVSCPTGVAHLFLAKLLPRFLLAHPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCC
VQVDLELTSRRVELIAEGIDVAIRVRSSLDDSGLILRSFGVSRQALVASPGFVHAHGPFA
CEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHCCCCCHHHCCCCCEEECCCCHH
SAAALHEVLGMGPRGVNGEPPRWRLTSPQGTSELVDYRSRLFTEDVELLHQAALAGMGVA
HHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLPLALCRGSIARGELVVLLGDHTLPVHQLHAVYPSRRGLAPGVRALIEFLVDVLPAVLE
HHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
SAEHEVSG
HHHHHCCC
>Mature Secondary Structure
MEDLNDFALLVAVVDHGSFSAAGRALGFPRSKVSRRVADLEQRLGVRLLQRSTRAVSLTE
CCCHHHHHHHHHHHHCCCCHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEHHHH
VGAEFYTHCKSITRSAQWALDVAERATQHPSGRIRVSCPTGVAHLFLAKLLPRFLLAHPD
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCC
VQVDLELTSRRVELIAEGIDVAIRVRSSLDDSGLILRSFGVSRQALVASPGFVHAHGPFA
CEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHCCCCCHHHCCCCCEEECCCCHH
SAAALHEVLGMGPRGVNGEPPRWRLTSPQGTSELVDYRSRLFTEDVELLHQAALAGMGVA
HHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QLPLALCRGSIARGELVVLLGDHTLPVHQLHAVYPSRRGLAPGVRALIEFLVDVLPAVLE
HHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
SAEHEVSG
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]