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The map label for this gene is leuC

Identifier: 78049142

GI number: 78049142

Start: 4116471

End: 4117919

Strand: Reverse

Name: leuC

Synonym: XCV3586

Alternate gene names: 78049142

Gene position: 4117919-4116471 (Counterclockwise)

Preceding gene: 78049144

Following gene: 78049141

Centisome position: 79.52

GC content: 66.05

Gene sequence:

>1449_bases
ATGACTGCCAAGACCCTGTACGACAAGCTGTGGGACCTGCATGAGGTCACACGCCGTGACGATGGTTCGTCGCTGATCTA
CATCGACCGCCACATCCTGCACGAGGTGACCTCGCCGCAAGCCTTCGAAGGCCTGCGCCTGGCCGGGCGCAAGCCATGGC
GCATCGACGCCAATATCGCCACGCCCGATCACAACGTGCCGACCACGCGCGCCGAGCGCCAGGGCGGTCTGGAGTCGATT
TCCGACGAGGTCTCGCGCCTGCAGGTGCAGACCCTGGACGAAAACTGCGACGACTTCGGCATCCTCGAGTTCAAGATGAA
CGATACCCGCCAGGGCATCGTGCACGTGGTCGGCCCCGAGCAGGGCGCCACCTTGCCGGGCATGACCGTGGTCTGCGGCG
ATTCGCACACGTCCACGCACGGCGCATTCGGCGCGCTGGCCCACGGCATCGGCACCTCGGAAGTCGAGCACGTGCTGGCC
ACCCAGTGCCTGATCGCCAAGAAGATGAAGAACATGCAGGTGCGCGTGGAAGGCACGCTGCCGTTCGGGGTGACCGCCAA
GGACATCGTGCTGGCGGTGATCGGCAAGATCGGCACCGCCGGCGGCAATGGCCATGCGCTGGAATTTGCCGGCAGTGCCA
TCCGCGCGGCGTCGATGGAAGGCCGCATGACCATCTGCAACATGGCCATCGAAGCCGGTGCCCGCGTGGGCATGGTGGCG
GTGGACGAGAAGACCATTGCCTACGTCAAAGGGCGGCCGTTCGCCCCCAAGGGCGCCCACTGGGATGCGGCGGTGGCGCT
CTGGAGCACGCTGGTCTCCGACCCGGACGCGCACTTCGACACCGTGGTGGAGTTGCGTGCCGAAGACATCAAGCCGCAGG
TCAGCTGGGGCACTTCGCCGGAAATGGTGCTGGCCATCGATCAGCACGTGCCCGATCCGGCCGCCGAGCAGGACCCGACC
AAGCGCGATTCGATCGAGCGCGCGCTCAAATACATGGGATTGACCGCCAACCAGCCGATCACCGCCATCCGCCTGGATCG
CGTATTCATCGGCTCGTGCACCAATTCGCGGATCGAAGACCTGCGTGCCGCCGCCGCAGTGGCCAAGGGCCGCAAGGTCG
CTTCCACCATCAAGCAGGCGTTGGTGGTGCCGGGCTCGGGCCTGGTGAAGGCCCAGGCCGAAGCCGAAGGCCTGGACAAG
GTGTTTCTGGACGCCGGCTTCGAATGGCGCGAGCCAGGCTGCTCGATGTGTCTGGCCATGAACCCGGACAAGCTGGGCAG
CGGCGAGCATTGCGCGTCCACCTCCAACCGCAACTTCGAAGGTCGCCAGGGCGCCGGCGGCCGCACGCATCTGGTCAGCC
CGGCGATGGCGGCGGCTGCGGCGGTGAGCGGGCATTTTGTCGATGTGCGTGAACTTGGAGATTCGGGAGTGGGGATTCGG
GATTCGTAG

Upstream 100 bases:

>100_bases
TCTTGAATAAACAATATGAATTTGCGTAATCCAAGGCAAGCTCCTAGCATCGATCTCAGCCAGGTGGGCGCACCCACCAT
CCGCCACTCGGATTGCCGCG

Downstream 100 bases:

>100_bases
AAGCGCTCCGCCCATCCGCAGCCTTTTCGAATCCCCAATCCCCAATCCCAAATCCCGGCCTCTCTCAATGACCCCTTTCA
CCCAACACACCGGACTGGTG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MTAKTLYDKLWDLHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTRAERQGGLESI
SDEVSRLQVQTLDENCDDFGILEFKMNDTRQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLIAKKMKNMQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRAASMEGRMTICNMAIEAGARVGMVA
VDEKTIAYVKGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSPEMVLAIDQHVPDPAAEQDPT
KRDSIERALKYMGLTANQPITAIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDK
VFLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
DS

Sequences:

>Translated_482_residues
MTAKTLYDKLWDLHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTRAERQGGLESI
SDEVSRLQVQTLDENCDDFGILEFKMNDTRQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLA
TQCLIAKKMKNMQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRAASMEGRMTICNMAIEAGARVGMVA
VDEKTIAYVKGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSPEMVLAIDQHVPDPAAEQDPT
KRDSIERALKYMGLTANQPITAIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDK
VFLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
DS
>Mature_481_residues
TAKTLYDKLWDLHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIATPDHNVPTTRAERQGGLESIS
DEVSRLQVQTLDENCDDFGILEFKMNDTRQGIVHVVGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLAT
QCLIAKKMKNMQVRVEGTLPFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRAASMEGRMTICNMAIEAGARVGMVAV
DEKTIAYVKGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSPEMVLAIDQHVPDPAAEQDPTK
RDSIERALKYMGLTANQPITAIRLDRVFIGSCTNSRIEDLRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKV
FLDAGFEWREPGCSMCLAMNPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIRD
S

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=28.6501377410468, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI41352693, Length=381, Percent_Identity=26.509186351706, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786259, Length=470, Percent_Identity=63.8297872340426, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787531, Length=366, Percent_Identity=26.5027322404372, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=23.5294117647059, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=373, Percent_Identity=28.4182305630027, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=375, Percent_Identity=28.8, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=315, Percent_Identity=27.6190476190476, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568399, Length=457, Percent_Identity=27.1334792122538, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=470, Percent_Identity=63.1914893617021, Blast_Score=604, Evalue=1e-173,
Organism=Saccharomyces cerevisiae, GI6323335, Length=362, Percent_Identity=27.9005524861878, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320440, Length=355, Percent_Identity=27.0422535211268, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=362, Percent_Identity=27.6243093922652, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI281365315, Length=400, Percent_Identity=27, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17864292, Length=400, Percent_Identity=27, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161076999, Length=400, Percent_Identity=27, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28571643, Length=375, Percent_Identity=26.6666666666667, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI17137564, Length=382, Percent_Identity=26.4397905759162, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_XANC5 (Q3BPJ6)

Other databases:

- EMBL:   AM039952
- RefSeq:   YP_365317.1
- ProteinModelPortal:   Q3BPJ6
- SMR:   Q3BPJ6
- STRING:   Q3BPJ6
- GeneID:   3729984
- GenomeReviews:   AM039952_GR
- KEGG:   xcv:XCV3586
- NMPDR:   fig|316273.3.peg.3585
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 51470; Mature: 51339

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTLYDKLWDLHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIA
CCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCEEEECEEC
TPDHNVPTTRAERQGGLESISDEVSRLQVQTLDENCDDFGILEFKMNDTRQGIVHVVGPE
CCCCCCCCCHHHHCCCHHHHHHHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLIAKKMKNMQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRAASMEGRMTICNMAIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCCEEEEHHHHHCCCCEEEEE
VDEKTIAYVKGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSP
ECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EMVLAIDQHVPDPAAEQDPTKRDSIERALKYMGLTANQPITAIRLDRVFIGSCTNSRIED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCHHHHH
LRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKVFLDAGFEWREPGCSMCLAM
HHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECHHHCCCHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHCCCCCCCCC
DS
CC
>Mature Secondary Structure 
TAKTLYDKLWDLHEVTRRDDGSSLIYIDRHILHEVTSPQAFEGLRLAGRKPWRIDANIA
CHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCEEEECEEC
TPDHNVPTTRAERQGGLESISDEVSRLQVQTLDENCDDFGILEFKMNDTRQGIVHVVGPE
CCCCCCCCCHHHHCCCHHHHHHHHHHEEHHHHHCCCCCCEEEEEEECCCCCCEEEEECCC
QGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQCLIAKKMKNMQVRVEGTL
CCCCCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECC
PFGVTAKDIVLAVIGKIGTAGGNGHALEFAGSAIRAASMEGRMTICNMAIEAGARVGMVA
CCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCCEEEEHHHHHCCCCEEEEE
VDEKTIAYVKGRPFAPKGAHWDAAVALWSTLVSDPDAHFDTVVELRAEDIKPQVSWGTSP
ECCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
EMVLAIDQHVPDPAAEQDPTKRDSIERALKYMGLTANQPITAIRLDRVFIGSCTNSRIED
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECEEEEECCCCHHHHH
LRAAAAVAKGRKVASTIKQALVVPGSGLVKAQAEAEGLDKVFLDAGFEWREPGCSMCLAM
HHHHHHHHCCHHHHHHHHHHHCCCCCCCEEECHHHCCCHHHHHHCCCCCCCCCCCEEEEE
NPDKLGSGEHCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVSGHFVDVRELGDSGVGIR
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHCCCEEEHHHCCCCCCCCC
DS
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA