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The map label for this gene is thiC [H]

Identifier: 78049131

GI number: 78049131

Start: 4098677

End: 4100554

Strand: Reverse

Name: thiC [H]

Synonym: XCV3575

Alternate gene names: 78049131

Gene position: 4100554-4098677 (Counterclockwise)

Preceding gene: 78049133

Following gene: 78049130

Centisome position: 79.18

GC content: 63.42

Gene sequence:

>1878_bases
ATGAATGCCGCGCCCACCGTTTTGCAACAACAAGCCCAGTCGTTGTCGGAGGCTGTCACCCAGCCGATTCCCGGTTCACG
CAAGATCTTCGTGCAAGGCTCGCGCGCCGATCTGCAGGTGCCGATGCGCGAGATCGCCTTGACGCGCACGCCCACCTTGT
TCGGCGGCGAAGAGAATCCGCCGCTCAGCGTCTACGACACGTCCGGGCCTTACACCGATCCGCGGGTGGCGATCGATCTG
GCAGCCGGTCTGGCACCGCTGCGTGCGCAGTGGATTGCCGAGCGTGGCGACACCGTCGCCCTGGACGGGCTGAGCTCCAG
CTTCGGGCGTGGCCGCGAGCACGATGTGCGTCTGGATGCAGTGCGCTTTCCGGCGCGGCGTCTGCCGCGGGTAGCGCGAC
AGGGCGCCAACGTCACCCAGATGCATTACGCGCGCCGCGGCATCATCACGCCGGAAATGGAGTACGTGGCGATCCGCGAA
AACCAGCGCCTGGAAGCGGTGACCGATGCAAGCTTGCGCAGGCAGCACCCGGGCCAGGCGTTCGGAGCTTCGATCCAGCA
ACGCATTACACCGGAGTTCGTGCGCGAAGAGATCGCACGGGGCCGCGCGATCTTGCCCAACAACATCAATCACCCCGAGA
GCGAGCCGATGATCATCGGCCGCAATTTCCTGACCAAGATCAATGCCAACATCGGCAACAGCGCGGTGTCTTCAGGCATT
GCCGAAGAGGTCGAGAAGCTGGTGTGGTCGATCCGCTGGGGCGGGGACACGGTCATGGATCTGTCCACCGGCAAGCACAT
CCATGAGACGCGCGAATGGATCATCCGCAACTCGCCGGTCCCGATCGGCACGGTGCCGATCTATCAGGCGCTGGAGAAGG
TCGATGGACGCGCCGAGGCGCTGACCTGGGAAATTTTTCGCGACACCTTGATCGAGCAGGCCGAACAGGGCGTGGATTAC
TTCACCATCCACGCCGGCGTGTTGCTGCGTTACGTACCGCTGACCGCACAGCGCGTGACCGGCATCGTCTCGCGTGGCGG
TTCGATCATGGCCAAGTGGTGCCTGGCGCATCACAAGGAGAATTTTCTCTACACGCACTTCGAAGACATCTGCCAGATCA
TGAAGGCCTACGACGTGGCGTTTTCGTTGGGCGATGGCCTACGCCCCGGCTGCATCGCCGACGCCAACGACGCGGCGCAA
TTCGGCGAGCTGGAAACGCTGGGCGAGCTGACCAAGCTCGCCTGGAAACACGATGTGCAGACCATGATTGAAGGCCCCGG
CCATGTGCCGATGCAATTGATCAAGGAAAACATGGACAAGCAGCTGCGCGAATGCGGCGAGGCGCCGTTCTACACCCTGG
GGCCGCTGACTACCGACATCGCGCCGGGCTACGACCACATCACCAGCGCGATCGGTGCGGCGATGATCGGCTGGTTCGGC
ACCGCCATGCTCTGCTACGTCACTCCGAAAGAGCACCTGGGCCTGCCCAACCGGCAGGACGTGCGCGACGGCATCATGGC
CTACAAGATCGCCGCGCATGCCGCCGACCTGGCCAAGGGGCATCCGGGCGCGCAAGTGCGCGACAACGCCTTGAGCAAGG
CGCGTTTCGAATTCCGTTGGGACGACCAGTTCCATCTTGGCCTGGATCCGGAAAAGGCCAAGGAGTTCCATGACCAAACC
CTGCCCAAGGACGCGCACAAGCTGGCGCACTTCTGCTCGATGTGCGGGCCACACTTCTGCTCGATGAAGATTACCCAGGA
CGTGCGTGATTACGCGGCCGAGCACGGCATGAGCGAGGCGCAAGCGCTGTCGACGGGCATGCAGGAAAAGTCCGCGCAAT
TTGTCGCGCAGGGCGCGCAGGTCTATCGCGCCACGTGA

Upstream 100 bases:

>100_bases
AGATGCGATGGTTCGTGCGTGCACGGGCAGTCGGTATCGAGCGCCGCCGCTTCGTCCGCACTGCGACTCCTTCGCAGGGC
CAGCACACAGGACGAATGCC

Downstream 100 bases:

>100_bases
CGCGTTACATGGCAGCGTTTCATAAAACGTGATGTAGCGCCTGCTTAGGAAAGGTTCAGTTTTGTACTGAACAGATTACG
GCGCGGTCCGCAAGTGCCGC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPRVAIDL
AAGLAPLRAQWIAERGDTVALDGLSSSFGRGREHDVRLDAVRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRRQHPGQAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAQRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDQT
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMSEAQALSTGMQEKSAQFVAQGAQVYRAT

Sequences:

>Translated_625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPRVAIDL
AAGLAPLRAQWIAERGDTVALDGLSSSFGRGREHDVRLDAVRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRRQHPGQAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAQRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDQT
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMSEAQALSTGMQEKSAQFVAQGAQVYRAT
>Mature_625_residues
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENPPLSVYDTSGPYTDPRVAIDL
AAGLAPLRAQWIAERGDTVALDGLSSSFGRGREHDVRLDAVRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRE
NQRLEAVTDASLRRQHPGQAFGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEALTWEIFRDTLIEQAEQGVDY
FTIHAGVLLRYVPLTAQRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQ
FGELETLGELTKLAWKHDVQTMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRWDDQFHLGLDPEKAKEFHDQT
LPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMSEAQALSTGMQEKSAQFVAQGAQVYRAT

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=607, Percent_Identity=68.5337726523888, Blast_Score=885, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 69187; Mature: 69187

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENP
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCEECCCCCC
PLSVYDTSGPYTDPRVAIDLAAGLAPLRAQWIAERGDTVALDGLSSSFGRGREHDVRLDA
CEEEEECCCCCCCCCEEHHHHHCHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCEEEHH
VRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRENQRLEAVTDASLRRQHPGQA
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHH
FGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
HHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHH
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEA
HHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
LTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAQRVTGIVSRGGSIMAKWCLAHHKE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQFGELETLGELTKLAWKHDVQ
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
TMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
HHHEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEE
DDQFHLGLDPEKAKEFHDQTLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMSEA
CCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCHHH
QALSTGMQEKSAQFVAQGAQVYRAT
HHHHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MNAAPTVLQQQAQSLSEAVTQPIPGSRKIFVQGSRADLQVPMREIALTRTPTLFGGEENP
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCEECCCCCC
PLSVYDTSGPYTDPRVAIDLAAGLAPLRAQWIAERGDTVALDGLSSSFGRGREHDVRLDA
CEEEEECCCCCCCCCEEHHHHHCHHHHHHHHHHHCCCEEEECCCHHHCCCCCCCCEEEHH
VRFPARRLPRVARQGANVTQMHYARRGIITPEMEYVAIRENQRLEAVTDASLRRQHPGQA
HHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHH
FGASIQQRITPEFVREEIARGRAILPNNINHPESEPMIIGRNFLTKINANIGNSAVSSGI
HHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCCEEECCHHHHHHCCCCCCHHHHHHH
AEEVEKLVWSIRWGGDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVDGRAEA
HHHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCHHHH
LTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAQRVTGIVSRGGSIMAKWCLAHHKE
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHCC
NFLYTHFEDICQIMKAYDVAFSLGDGLRPGCIADANDAAQFGELETLGELTKLAWKHDVQ
CCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHH
TMIEGPGHVPMQLIKENMDKQLRECGEAPFYTLGPLTTDIAPGYDHITSAIGAAMIGWFG
HHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHH
TAMLCYVTPKEHLGLPNRQDVRDGIMAYKIAAHAADLAKGHPGAQVRDNALSKARFEFRW
HHHEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCEEEEEE
DDQFHLGLDPEKAKEFHDQTLPKDAHKLAHFCSMCGPHFCSMKITQDVRDYAAEHGMSEA
CCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHCCCHHH
QALSTGMQEKSAQFVAQGAQVYRAT
HHHHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]