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The map label for this gene is mpl [H]

Identifier: 78049108

GI number: 78049108

Start: 4068458

End: 4069816

Strand: Reverse

Name: mpl [H]

Synonym: XCV3552

Alternate gene names: 78049108

Gene position: 4069816-4068458 (Counterclockwise)

Preceding gene: 78049109

Following gene: 78049107

Centisome position: 78.59

GC content: 66.81

Gene sequence:

>1359_bases
ATGAGCAAACTCCACATTCTCGGCATCGCCGGAACTTTCATGGGCGGTGTGGCCGCCCTGGCGCGTGAGCTGGGCTGGCA
GGTGGAAGGCAGCGACCAGGCCATTTATCCGCCGATGTCCACACAGCTGGAAACGCTGGGCATTGCGTTGGCGCAGGGCT
ATGCGCCGTCCAATATTTCGCCGGATGCGACCGACGTGGTTGTCGGTAATGCGCTTTCGCGCGGCAATGCGGCGGTGGAG
GCGGTGCTGGATGCCGGCCGCCGGTACACCTCCGGCGCGCAATGGCTGGCCGAGCAGGTATTGCCCGGCCGCGACACGCT
GGCCGTGGCCGGCACCCACGGCAAGACCACCACCACCACCATCCTGAGCTACCTGCTGGAGGCGGCCGGGCGCGCGCCCG
GGTTTCTGATCGGTGGTGTGGCCGAAGATTTCGGCGTGTCGGCGCGACTGGGCCAGGGGCGCGAATTCGTGGTGGAAGCC
GACGAATACGACACCGCGTTTTTCGACAAGCGCAGCAAGTTCGTGCACTACCGGCCGTTGGTGGCGATCCTCAATAACCT
GGAATACGACCACGCCGACATCTTCCCGGACGTGGCGGCCATCCAGCGCCAGTTCCACCACCTGGTGCGCACGGTGCCCG
CGCGCGGTCGCCTGATCGTCAACGGCGAAGACGCGCACCTGGCCGAGGTGCTGGCGATGGGATGCTGGACGCCGGTGGAG
CGGTTCGGGTTCGATGCGTCGCTGGACTGGAGCGCGCGGTTGATCGCGGCCGATGGCAGCGCGTTTACGGTGCTGCACCG
TGGCGTGGAAATTGGCCAGGTGCAGTGGCCCCTGGTTGGGCGGCATAACGTCCTGAACGGGTTGGCGGCGCTGGCAGCAG
TACAGGCGGTAGGCGTGGATCCGGCGACGGTGATGCCGGCATTGGCACAGTTCCAGAGCGTCAAGCGGCGGCTGGAAGTC
ATCGGTAGCGCGCGCGCGATCACCGTGTACGACGACTTTGCGCACCATCCCACCGCCATCGCGACGACGTTGCAGGGTCT
GCGTGCGAAGGTCGGTGCGGCGCGTGTTGTGGTGGCAATGGAGCCGCGCAGCAATTCGATGCGGCTGGGCGCGCATGCCC
AGGCGCTGGCGCCGTCGTTGCACGATGCCGATGCAGTGGTGTTTCTGCATCGGCCGGAACTGGCCTGGGACGCGGCACCG
ATCATTGCGCAGGTGCGCGGCGAGGCGCGCGTGGTGCAGGACGTGGATGCCTTGCTGCGCGCGCTGGGCGAAATTGCCCG
GCCCGGAGACCATGTGGTGTTCATGTCCAACGGTGGCTTCGACGGTGCGCCGCGTCGTTTCCTGGCGCAGTTGTCCTGA

Upstream 100 bases:

>100_bases
ATCGGGAAATTCCCGACAGTACGGTAGGAAATCCCCGACACTTGGGCCTGCGTTGGAATGGAATGTCCGGCAACTGCGGC
GATCGGCGACAATGGGCGGC

Downstream 100 bases:

>100_bases
TGGTGCCGATCCCCGCCACTGCAGACACCAGCGCGTTGCCGCTGTTCCCCTTGCACAGCGTGCTGTTGCCTGGCGCGGCG
ATGGGCTTGCGCGTGTTTGA

Product: UDP-N-acetylmuramate--L-alanine ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISPDATDVVVGNALSRGNAAVE
AVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEA
DEYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
RFGFDASLDWSARLIAADGSAFTVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVDPATVMPALAQFQSVKRRLEV
IGSARAITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAP
IIAQVRGEARVVQDVDALLRALGEIARPGDHVVFMSNGGFDGAPRRFLAQLS

Sequences:

>Translated_452_residues
MSKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISPDATDVVVGNALSRGNAAVE
AVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEA
DEYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
RFGFDASLDWSARLIAADGSAFTVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVDPATVMPALAQFQSVKRRLEV
IGSARAITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAP
IIAQVRGEARVVQDVDALLRALGEIARPGDHVVFMSNGGFDGAPRRFLAQLS
>Mature_451_residues
SKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNISPDATDVVVGNALSRGNAAVEA
VLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTTILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEAD
EYDTAFFDKRSKFVHYRPLVAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVER
FGFDASLDWSARLIAADGSAFTVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVDPATVMPALAQFQSVKRRLEVI
GSARAITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAMEPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPI
IAQVRGEARVVQDVDALLRALGEIARPGDHVVFMSNGGFDGAPRRFLAQLS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=451, Percent_Identity=52.7716186252772, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1786279, Length=355, Percent_Identity=34.6478873239437, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48170; Mature: 48039

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNIS
CCCEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
PDATDVVVGNALSRGNAAVEAVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTT
CCCHHEEECCCHHCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
ILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEADEYDTAFFDKRSKFVHYRPL
HHHHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCEEEHHHH
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHH
RFGFDASLDWSARLIAADGSAFTVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVD
HCCCCCCCCCCEEEEEECCCEEEEECCCCEECCEECCCCCCHHHHHHHHHHHHHHHCCCC
PATVMPALAQFQSVKRRLEVIGSARAITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAM
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
EPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPIIAQVRGEARVVQDVDALLR
CCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
ALGEIARPGDHVVFMSNGGFDGAPRRFLAQLS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHCC
>Mature Secondary Structure 
SKLHILGIAGTFMGGVAALARELGWQVEGSDQAIYPPMSTQLETLGIALAQGYAPSNIS
CCEEEEEEHHHHHHHHHHHHHHHCCEECCCCCEECCCCCHHHHHHHHHHHCCCCCCCCC
PDATDVVVGNALSRGNAAVEAVLDAGRRYTSGAQWLAEQVLPGRDTLAVAGTHGKTTTTT
CCCHHEEECCCHHCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHH
ILSYLLEAAGRAPGFLIGGVAEDFGVSARLGQGREFVVEADEYDTAFFDKRSKFVHYRPL
HHHHHHHHHCCCCCEEEECCHHHCCCCCCCCCCCEEEEECCCCCHHHHHCCCCEEEHHHH
VAILNNLEYDHADIFPDVAAIQRQFHHLVRTVPARGRLIVNGEDAHLAEVLAMGCWTPVE
HHHHHCCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHH
RFGFDASLDWSARLIAADGSAFTVLHRGVEIGQVQWPLVGRHNVLNGLAALAAVQAVGVD
HCCCCCCCCCCEEEEEECCCEEEEECCCCEECCEECCCCCCHHHHHHHHHHHHHHHCCCC
PATVMPALAQFQSVKRRLEVIGSARAITVYDDFAHHPTAIATTLQGLRAKVGAARVVVAM
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEEE
EPRSNSMRLGAHAQALAPSLHDADAVVFLHRPELAWDAAPIIAQVRGEARVVQDVDALLR
CCCCCCEEECCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHHHHHHHH
ALGEIARPGDHVVFMSNGGFDGAPRRFLAQLS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]