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The map label for this gene is btuB [H]

Identifier: 78048879

GI number: 78048879

Start: 3799932

End: 3801809

Strand: Reverse

Name: btuB [H]

Synonym: XCV3323

Alternate gene names: 78048879

Gene position: 3801809-3799932 (Counterclockwise)

Preceding gene: 78048880

Following gene: 78048878

Centisome position: 73.42

GC content: 65.44

Gene sequence:

>1878_bases
ATGTCCCTTTCTTCCGTTTCGCCGCGTCGTGCCGTGCTCGTCGTCGGGCTGTCGCTGTGCGCTGCCACTGCGGCTCAGGC
CGAGGCCGCCATCGATCTGGACCAAGTCGTGGTCACCGCCTCGCGCACCGCACAGACCCAGGACCAGGCCCTGGCACCGG
TCACGGTGATCGACCGCGCACAGATCGAGCGTCGTCAGGTCAATTCGCTGCAGGACCTGTTGCGCGGCGAAGCGGGCGTG
TCGCTGGCCAACAATGGCGGACCCGGCAAGGCGACCTCGCTGTTCCTGCGCGGCACCGAGGCCGACCAGGTGGTGGTGCT
GATCGATGGCGTGCGCATCGGCTCGGCGACCGCCGGCGGTGCGGCGCTGCAGGATGTGCCGATCGAGCAGATCGAACGCA
TCGAAATCGTGCGTGGCCCGTTTTCCAGCCTGTATGGCTCCGAAGCGCTGGGCGGTGTGATCCAGATCTTTACCCGACGC
CCGCAAGGCAGTGTCGTGCCGACCTTGAGCGTGGCGGCCGGTAGCGACAACGCGCGCCGCTACGGCGCGGGCATCGCCGG
GCGCAGCCCGGGCGATCTGAGCGATGCCGGTGGTTGGTATTCGGTGAACGCCGTGCACGACGAAACCGACGGTATCAACG
CGTATCTGGATACCAGCTCCAGCGCCTATGACCCGGACCGCGACGGCTATCGCAACGATTCGTTGAGCGCGCAGGGCGGC
TGGCGTTTCAATCGCCAGTGGGATGCCGATGTGCATGCAGTGCGCGCGCAGAGCCGTAACGAATACGACGGTTCGGCATT
CGGCGGCAATCTGGGCAAGGGCGTGCAGCAGGCCATCGGCGGCCGCGTGCGGTACGCGCCGAACGAGGCGCTCACGCTCA
CTGCCAGCGTGGGCAGCAGCGCCGATCTGAGCGATGCGTATTACCAAGGCGCCTACCTGTCGACCTACGACACCCGCCGC
AAGCAGGGTGCGTTGCAGGCCGATCTCGACACCGGCCCCGGTCTGCTCACGGTCGGCTTCGATTGGCAGCGGGACGCCAT
CGCCAGCAGCGACAATTACAATGCCGACAGCCGCATCGACCGCGCCGCATTTGCGCAGTGGCAGCAGACTTTTGGCAGTC
AATCGCTGCAAGCCAGCCTGCGCCGCAACGACAACAGCCAGTTCGGCGGCAAGACTACCGGCAGCCTGCTGTGGGGTTGG
GATTTTGCCGAGCATCTGCGCCTGACGGCCAGCTACGGCACTGCGTTCAAGGCGCCCACCTTCAACGAGTTGTACTACCC
CGATTACGGCAACCCGCTGCTCGGGCCGGAAACCTCCAAGAGTGCCGAACTCGGTCTGCGCGGTCGCTACGATTGGGGCA
CCTGGACGCTCAGCGCGTTCCAGACCCGTATCGACGATCTCATCGCCTACGACGCGTCGTTGGTGGATGCCACGCACCCG
TTCGGGCAGCCGAACAATATCGACCGGGCGCGCATCCGCGGCGTGGAAGCCGGCTACGACACCGTGCTGGCCGGCTGGAC
CGTGCGCAGCGCAGTGACCTGGCTTGCGCCGCAGGCCGATGGTGAAGTCAATCATGGCAATTGGTTGCCGCGGCGTGCGC
GCCAGAGCGGGCGTATCGATGCCGACCGCAGCATCGGTGCATTCGGCATTGGCGCAAGCCTGTTCGGTTCGGGTGCTCGC
TACGACGATCTGGCCAATACCGATCGGTTGGCCGGCTACGGCTTGCTGGATCTGCGCGTGAGTTATGCAGTGAATGCGGA
CTGGAAGGTGCAATTTGCCGCCAACAATGTGTTCGACCGCCACTACGAAACCGCACGCTGGTATGCCCAGCCACGCCGCA
ATTATCTGCTGACCTTCCGCTACCAACCGGCGCACTGA

Upstream 100 bases:

>100_bases
GCCTTGCTCGTCTGACGCTGCGCGCCGGACGCCTGGCCCGTTGCTGCTTGCCGCCCGATTGCCCACGCGCGGGTTGGCGC
GGTTCCAGGAGCAGCAGTTG

Downstream 100 bases:

>100_bases
GAGCAGCCGACGACACGACCGCGCAGCCACCGCATGGTTGCGGGCGGTGCCCGGATCCGCATGGACCAGGCGGATACTGC
GTTTGCTCCTGCTGTCCGCA

Product: TonB-dependent outer membrane receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSLSSVSPRRAVLVVGLSLCAATAAQAEAAIDLDQVVVTASRTAQTQDQALAPVTVIDRAQIERRQVNSLQDLLRGEAGV
SLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGGAALQDVPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRR
PQGSVVPTLSVAAGSDNARRYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSLSAQGG
WRFNRQWDADVHAVRAQSRNEYDGSAFGGNLGKGVQQAIGGRVRYAPNEALTLTASVGSSADLSDAYYQGAYLSTYDTRR
KQGALQADLDTGPGLLTVGFDWQRDAIASSDNYNADSRIDRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGW
DFAEHLRLTASYGTAFKAPTFNELYYPDYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATHP
FGQPNNIDRARIRGVEAGYDTVLAGWTVRSAVTWLAPQADGEVNHGNWLPRRARQSGRIDADRSIGAFGIGASLFGSGAR
YDDLANTDRLAGYGLLDLRVSYAVNADWKVQFAANNVFDRHYETARWYAQPRRNYLLTFRYQPAH

Sequences:

>Translated_625_residues
MSLSSVSPRRAVLVVGLSLCAATAAQAEAAIDLDQVVVTASRTAQTQDQALAPVTVIDRAQIERRQVNSLQDLLRGEAGV
SLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGGAALQDVPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRR
PQGSVVPTLSVAAGSDNARRYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSLSAQGG
WRFNRQWDADVHAVRAQSRNEYDGSAFGGNLGKGVQQAIGGRVRYAPNEALTLTASVGSSADLSDAYYQGAYLSTYDTRR
KQGALQADLDTGPGLLTVGFDWQRDAIASSDNYNADSRIDRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGW
DFAEHLRLTASYGTAFKAPTFNELYYPDYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATHP
FGQPNNIDRARIRGVEAGYDTVLAGWTVRSAVTWLAPQADGEVNHGNWLPRRARQSGRIDADRSIGAFGIGASLFGSGAR
YDDLANTDRLAGYGLLDLRVSYAVNADWKVQFAANNVFDRHYETARWYAQPRRNYLLTFRYQPAH
>Mature_624_residues
SLSSVSPRRAVLVVGLSLCAATAAQAEAAIDLDQVVVTASRTAQTQDQALAPVTVIDRAQIERRQVNSLQDLLRGEAGVS
LANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGGAALQDVPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRRP
QGSVVPTLSVAAGSDNARRYGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSLSAQGGW
RFNRQWDADVHAVRAQSRNEYDGSAFGGNLGKGVQQAIGGRVRYAPNEALTLTASVGSSADLSDAYYQGAYLSTYDTRRK
QGALQADLDTGPGLLTVGFDWQRDAIASSDNYNADSRIDRAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGWD
FAEHLRLTASYGTAFKAPTFNELYYPDYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATHPF
GQPNNIDRARIRGVEAGYDTVLAGWTVRSAVTWLAPQADGEVNHGNWLPRRARQSGRIDADRSIGAFGIGASLFGSGARY
DDLANTDRLAGYGLLDLRVSYAVNADWKVQFAANNVFDRHYETARWYAQPRRNYLLTFRYQPAH

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=636, Percent_Identity=33.0188679245283, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI1788478, Length=543, Percent_Identity=27.0718232044199, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1786798, Length=168, Percent_Identity=30.3571428571429, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67548; Mature: 67417

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLSSVSPRRAVLVVGLSLCAATAAQAEAAIDLDQVVVTASRTAQTQDQALAPVTVIDRA
CCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHCCCCEEEHHHH
QIERRQVNSLQDLLRGEAGVSLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGG
HHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCCC
AALQDVPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRRPQGSVVPTLSVAAGSDNARR
CHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHH
YGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSLSAQGG
HCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
WRFNRQWDADVHAVRAQSRNEYDGSAFGGNLGKGVQQAIGGRVRYAPNEALTLTASVGSS
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCC
ADLSDAYYQGAYLSTYDTRRKQGALQADLDTGPGLLTVGFDWQRDAIASSDNYNADSRID
CCCCHHHHCCCEEEHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHCCCCCCCCHHHHH
RAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGWDFAEHLRLTASYGTAFKAPT
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHEEEEECCCCEECCCC
FNELYYPDYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATHP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHCCCC
FGQPNNIDRARIRGVEAGYDTVLAGWTVRSAVTWLAPQADGEVNHGNWLPRRARQSGRID
CCCCCCCCHHHHEECCCCCCCEEECCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCC
ADRSIGAFGIGASLFGSGARYDDLANTDRLAGYGLLDLRVSYAVNADWKVQFAANNVFDR
CCCCCCEEECCHHHHCCCCCCCCCCCCCHHCCCEEEEEEEEEEECCCEEEEEECCCHHHH
HYETARWYAQPRRNYLLTFRYQPAH
HHHHHHHHHCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SLSSVSPRRAVLVVGLSLCAATAAQAEAAIDLDQVVVTASRTAQTQDQALAPVTVIDRA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHEEEECCCCCCCHHHCCCCEEEHHHH
QIERRQVNSLQDLLRGEAGVSLANNGGPGKATSLFLRGTEADQVVVLIDGVRIGSATAGG
HHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEEECCCCCEEEEEEECEEECCCCCCC
AALQDVPIEQIERIEIVRGPFSSLYGSEALGGVIQIFTRRPQGSVVPTLSVAAGSDNARR
CHHCCCCHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHH
YGAGIAGRSPGDLSDAGGWYSVNAVHDETDGINAYLDTSSSAYDPDRDGYRNDSLSAQGG
HCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
WRFNRQWDADVHAVRAQSRNEYDGSAFGGNLGKGVQQAIGGRVRYAPNEALTLTASVGSS
EEECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCC
ADLSDAYYQGAYLSTYDTRRKQGALQADLDTGPGLLTVGFDWQRDAIASSDNYNADSRID
CCCCHHHHCCCEEEHHHHHHHCCCEEEECCCCCCEEEECCCCCCHHHCCCCCCCCHHHHH
RAAFAQWQQTFGSQSLQASLRRNDNSQFGGKTTGSLLWGWDFAEHLRLTASYGTAFKAPT
HHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHEEEEECCCCEECCCC
FNELYYPDYGNPLLGPETSKSAELGLRGRYDWGTWTLSAFQTRIDDLIAYDASLVDATHP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCHHHHCCCC
FGQPNNIDRARIRGVEAGYDTVLAGWTVRSAVTWLAPQADGEVNHGNWLPRRARQSGRID
CCCCCCCCHHHHEECCCCCCCEEECCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCC
ADRSIGAFGIGASLFGSGARYDDLANTDRLAGYGLLDLRVSYAVNADWKVQFAANNVFDR
CCCCCCEEECCHHHHCCCCCCCCCCCCCHHCCCEEEEEEEEEEECCCEEEEEECCCHHHH
HYETARWYAQPRRNYLLTFRYQPAH
HHHHHHHHHCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA