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The map label for this gene is metB [H]

Identifier: 78048732

GI number: 78048732

Start: 3608088

End: 3609305

Strand: Reverse

Name: metB [H]

Synonym: XCV3176

Alternate gene names: 78048732

Gene position: 3609305-3608088 (Counterclockwise)

Preceding gene: 78048733

Following gene: 78048731

Centisome position: 69.7

GC content: 64.61

Gene sequence:

>1218_bases
ATGAGCTTTCGTGACCCCACCGACGCCCCCTGTACTGCTGCCACCGCCGCCGTGCGTGCCGGTATCGATCGCGACACCGC
CTACGGGGCAGTGACCCCGCCGATCGTGCTGTCGTCGAATTTCTCGTTCGATGGTTTCGGCAACAAGCGCCAGTACGACT
ACACCCGCAGCGGCAATCCGACCCGCGACCTGTTAGGCGAAGCGCTGGCCGAATTGGAAGGCGGCGCGGGCGGCGTGATC
ACCTCCACCGGCATGGGCGCGATCAATCTGGTGCTCAACGCGGTGCTGCAACCCGGCGATACGCTGGTGGTGCCGCACGA
TGCGTACGGCGGCAGCTGGCGCCTGTTCAATGCATTGGCCAAGAAAGGCCACTTCGCGCTGATCACCGCGGATCTGACCG
ATCCGCGTTCGCTGGCCGACGCATTGGCGCAGTCGCCCAAGCTGGTGCTGATCGAAACCCCGTCCAATCCGCTGCTGCGC
ATCACCGATCTGCGCTTTGTCATCGAAGCTGCAAGGAAGGTCGGCGCATTGACGGTGGTGGACAACACCTTCCTGTCTCC
GGCGCTGCAGAAGCCGCTGGATTTCGGTGCCGATCTGGTTCTGCACTCCACCACCAAGTACATCAACGGCCATAGCGATG
TGGTGGGCGGTGCGGTGGTGGCACGCGATGCCCAACTGCATCAGCAACTGGTGTGGTGGGCCAATGCGTTGGGCCTGACC
GGCTCGCCGTTCGATGCCTTCCTTACCTTGCGCGGTCTGCGCACGCTGGATGCCCGGCTGCGCGTGCATCAGGAAAACGC
CGATGCGATCGCTGCATTGCTGGACGGGCATGCGGCGGTGAATCGGGTGTATTTCCCGGGGCTGGCCTCGCATCCGGGCC
ATGCACTGGCCGCGCGTCAGCAGAAGGGCTTCGGGGCGATGATGAGTTTCGAACTGGAAGGCGGCGAAGCGGCCGTGCGT
GCGTTCGTCGATGGCTTGCGTTACTTCACCCTGGCCGAATCGTTGGGCGGAGTGGAAAGCCTGATCGCGCATCCGGCCTC
GATGACGCATGCGGCAATGACCGCCGAAGCGCGTGCGGCAGCCGGTATTTCCGATGGCTTGCTGCGCTTGTCGATCGGGA
TCGAATCGGCCGACGATCTGCTGATCGATCTGCGCGCCGGCCTGGCCCGTGCCGAAGCGACCCTGACCGCCGCCAGCCGC
AAACAGGTCGACGCGTGA

Upstream 100 bases:

>100_bases
GCGTGTTGCGCTCGCCGTATGGGCACGACGCCTTCCTGAAAGAAATCGATCGCATCGACGCAATCCTCACTACCGCCCTT
CGCAGCACCGGAGAAACCGC

Downstream 100 bases:

>100_bases
GCAATGTCCTGCCCGTTTCGCGCCGCGCTCCGGTCGCTGCAGTGACGCCCACACCGCGCCTAGCCTTGCTGGGGACCGGC
ACCGTGGGGCGCGCGTTCGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSFRDPTDAPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVI
TSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLR
ITDLRFVIEAARKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNRVYFPGLASHPGHALAARQQKGFGAMMSFELEGGEAAVR
AFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESADDLLIDLRAGLARAEATLTAASR
KQVDA

Sequences:

>Translated_405_residues
MSFRDPTDAPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVI
TSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLR
ITDLRFVIEAARKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNRVYFPGLASHPGHALAARQQKGFGAMMSFELEGGEAAVR
AFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESADDLLIDLRAGLARAEATLTAASR
KQVDA
>Mature_404_residues
SFRDPTDAPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNPTRDLLGEALAELEGGAGGVIT
STGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALAKKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLRI
TDLRFVIEAARKVGALTVVDNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLTG
SPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNRVYFPGLASHPGHALAARQQKGFGAMMSFELEGGEAAVRA
FVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAAAGISDGLLRLSIGIESADDLLIDLRAGLARAEATLTAASRK
QVDA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=40.8839779005525, Blast_Score=271, Evalue=6e-73,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=38.6740331491713, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=36.8421052631579, Blast_Score=217, Evalue=1e-56,
Organism=Escherichia coli, GI1790375, Length=375, Percent_Identity=59.4666666666667, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=33.587786259542, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17538055, Length=365, Percent_Identity=44.6575342465753, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI17560056, Length=348, Percent_Identity=44.8275862068966, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=35.1351351351351, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=42.8940568475452, Blast_Score=276, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6321254, Length=402, Percent_Identity=31.8407960199005, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=28.110599078341, Blast_Score=139, Evalue=8e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=28.894472361809, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=325, Percent_Identity=29.2307692307692, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6323557, Length=251, Percent_Identity=27.4900398406374, Blast_Score=73, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322970, Length=272, Percent_Identity=26.1029411764706, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922554, Length=397, Percent_Identity=41.5617128463476, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 42570; Mature: 42439

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRDPTDAPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNP
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVITSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
KKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLRITDLRFVIEAARKVGALTVV
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEE
DNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
CCCHHCHHHHCCCCCCCCEEEECCHHHCCCCCCHHCCHHEEHHHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNRVYFPGLASHPGHALAARQ
CCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHCEEECCCCCCCCCCHHHHHH
QKGFGAMMSFELEGGEAAVRAFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AGISDGLLRLSIGIESADDLLIDLRAGLARAEATLTAASRKQVDA
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SFRDPTDAPCTAATAAVRAGIDRDTAYGAVTPPIVLSSNFSFDGFGNKRQYDYTRSGNP
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
TRDLLGEALAELEGGAGGVITSTGMGAINLVLNAVLQPGDTLVVPHDAYGGSWRLFNALA
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
KKGHFALITADLTDPRSLADALAQSPKLVLIETPSNPLLRITDLRFVIEAARKVGALTVV
CCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCEEEEHHHHHHHHHHHHCCEEEEE
DNTFLSPALQKPLDFGADLVLHSTTKYINGHSDVVGGAVVARDAQLHQQLVWWANALGLT
CCCHHCHHHHCCCCCCCCEEEECCHHHCCCCCCHHCCHHEEHHHHHHHHHHHHHHHCCCC
GSPFDAFLTLRGLRTLDARLRVHQENADAIAALLDGHAAVNRVYFPGLASHPGHALAARQ
CCCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCHHHHCEEECCCCCCCCCCHHHHHH
QKGFGAMMSFELEGGEAAVRAFVDGLRYFTLAESLGGVESLIAHPASMTHAAMTAEARAA
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
AGISDGLLRLSIGIESADDLLIDLRAGLARAEATLTAASRKQVDA
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]