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The map label for this gene is gltX [H]

Identifier: 78048563

GI number: 78048563

Start: 3420015

End: 3421418

Strand: Reverse

Name: gltX [H]

Synonym: XCV3007

Alternate gene names: 78048563

Gene position: 3421418-3420015 (Counterclockwise)

Preceding gene: 78048564

Following gene: 78048562

Centisome position: 66.07

GC content: 65.17

Gene sequence:

>1404_bases
ATGACCTGCCGCACCCGTTTCGCCCCCAGCCCCACCGGTTACCTGCACATCGGCGGCGCCCGCACCGCCCTGTATTGCTG
GCTGGAGGCGCGCCACCGCGGCGGGCAGTTCGTGCTGCGCATCGAAGACACCGACCGCGAGCGCAGCACCCAGGCGGCCA
TCGACGCCATCCTGGAAGCGATGGACTGGCTGGGCCTGGGCTACGACGAAGGCCCGATCTACCAGACCCAGCGCATCGCG
CGCTACCAGGAGGTTGCCGAGCAGTTGCTCGCGCAGGGCAAGGCGTATTACGCCTACGAAACCCGCGAAGAGCTCGACGC
CATGCGCGAGGCCGCCATGGCCAAGCAGGAAAAGCCGCGTTTCAACGGCGCTGCCCGCGAGCAGAACCTGCCGTATCGCG
ACGACCCCAACCGGGTCATCCGCTTCAAGAATCCCATCGGCGGCACGGTAGTGTTCGAGGACCTGATCAAGGGGTGCATC
GAGATCGCCAACAGCGAGCTCGACGACATGGTGATCTTCCGCCCGGACGGCTTCCCCACCTACAACTTCGCAGTGGTGGT
GGACGACTGGGACATGGGCATCACCGAGGTGATCCGCGGCGACGACCACATCAACAACACTCCGCGCCAGATCAATATCT
ACGAAGCACTGGGCGCACCGGTGCCCAGGTTCGCGCACATGCCGATGATCCTGGACGAGCAGGGCGCCAAGCTGTCCAAG
CGCACCGGCGCGGCCGACGTCATGCAGTACAAGGACGCCGGCTATCTGCCGCATGCGCTGATAAATTACCTGGCGCGCCT
GGGCTGGTCGCACGGCGACCAAGAGCTGTTCACCCGGCAGGAATTGCTGGACCTGTTCGACGTCAAGGACGTCAATTCCA
AGGCCGCGCGCCTGGACATGGCCAAGCTCGGCTGGGTCAATCAGCACTACCTGAAGACCGACGACCCGGCCAGCATCGCC
CCGCAGTTGGAATACCAGCTTGCCAAGCTCGGTGTGGACATCGCCGCCGGCCCGGCCGCCGCCGATGTGGTGGTGGCCTT
GCGCGAGCGCGTGCAGACCTTGAAGGAAATGGCCGAAAAAGCCGTGGTCTGGTACCAGCCGCTGGAAACCTATGACGAAG
CGGCGGTGATGAAACACCTCAAGCTTGGCGCCGAGGTGCCGCTGGGCAAGGCGCGCGAGCTGCTGGCTGCCGTGGATGCG
TGGAGCGTCGAAAACGTCTCGGCTGCGCTGCACGACGCTGCTGCCGCGCTGGAACTGGGCATGGGCAAGATCGCCCAGCC
GCTGCGCGTGGCCATCACCGGCACCCAGGTCAGCCCGGATATTTCGCAAACGGTGTACCTGGCCGGACGCGAGGGTGCCT
TGAAACGCATCGATGCGGCACTCATCAAGATTGGAGCGGCCTGA

Upstream 100 bases:

>100_bases
GTGGTGTACGGCGACCTGCTGCACAACCGCTGAGCCACGGGCCGGGCAGGGCCACGCTGCGCAAGGTAAAATCGGCCGAC
TCCCGTCCGCCGAGCGCTCC

Downstream 100 bases:

>100_bases
CGCCGGAGCCTGTATGACCAAGCACACCCACGCACCAGATCTCGCCTGCACTGCACCGCATCACCACGTCGACGATGCCA
ACGGTTTCGTGCGCGCGGTG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQAAIDAILEAMDWLGLGYDEGPIYQTQRIA
RYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRFNGAAREQNLPYRDDPNRVIRFKNPIGGTVVFEDLIKGCI
EIANSELDDMVIFRPDGFPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALGAPVPRFAHMPMILDEQGAKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTRQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIA
PQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEKAVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDA
WSVENVSAALHDAAAALELGMGKIAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA

Sequences:

>Translated_467_residues
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQAAIDAILEAMDWLGLGYDEGPIYQTQRIA
RYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRFNGAAREQNLPYRDDPNRVIRFKNPIGGTVVFEDLIKGCI
EIANSELDDMVIFRPDGFPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALGAPVPRFAHMPMILDEQGAKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTRQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIA
PQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEKAVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDA
WSVENVSAALHDAAAALELGMGKIAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA
>Mature_466_residues
TCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQAAIDAILEAMDWLGLGYDEGPIYQTQRIAR
YQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPRFNGAAREQNLPYRDDPNRVIRFKNPIGGTVVFEDLIKGCIE
IANSELDDMVIFRPDGFPTYNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALGAPVPRFAHMPMILDEQGAKLSKR
TGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTRQELLDLFDVKDVNSKAARLDMAKLGWVNQHYLKTDDPASIAP
QLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEKAVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDAW
SVENVSAALHDAAAALELGMGKIAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=326, Percent_Identity=36.5030674846626, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=54.0130151843818, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=38.4297520661157, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71993394, Length=473, Percent_Identity=28.9640591966173, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324540, Length=499, Percent_Identity=30.8617234468938, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=29.3023255813954, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=31.4629258517034, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51815; Mature: 51683

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQAAIDAILEA
CCCCCCCCCCCCCEEEECCCCEEHEEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHH
MDWLGLGYDEGPIYQTQRIARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCC
FNGAAREQNLPYRDDPNRVIRFKNPIGGTVVFEDLIKGCIEIANSELDDMVIFRPDGFPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALGAPVPRFAHMPMILDEQGAKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEHHHHCCCCCHHHCCCEEECCCCCCHHH
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTRQELLDLFDVKDVNSKAARLDM
HCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKLGWVNQHYLKTDDPASIAPQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEK
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDAWSVENVSAALHDAAAALELG
HEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKIAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA
HHHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQAAIDAILEA
CCCCCCCCCCCCEEEECCCCEEHEEEHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHH
MDWLGLGYDEGPIYQTQRIARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCC
FNGAAREQNLPYRDDPNRVIRFKNPIGGTVVFEDLIKGCIEIANSELDDMVIFRPDGFPT
CCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCC
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALGAPVPRFAHMPMILDEQGAKLSK
EEEEEEEECCCCCHHHHHCCCCCCCCCCCEEEEHHHHCCCCCHHHCCCEEECCCCCCHHH
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFTRQELLDLFDVKDVNSKAARLDM
HCCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
AKLGWVNQHYLKTDDPASIAPQLEYQLAKLGVDIAAGPAAADVVVALRERVQTLKEMAEK
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVVWYQPLETYDEAAVMKHLKLGAEVPLGKARELLAAVDAWSVENVSAALHDAAAALELG
HEEEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
MGKIAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA
HHHHCCCEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]