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The map label for this gene is yjbJ [H]

Identifier: 78048191

GI number: 78048191

Start: 2970519

End: 2971502

Strand: Reverse

Name: yjbJ [H]

Synonym: XCV2635

Alternate gene names: 78048191

Gene position: 2971502-2970519 (Counterclockwise)

Preceding gene: 78048192

Following gene: 78048190

Centisome position: 57.38

GC content: 66.57

Gene sequence:

>984_bases
ATGAAGGGGATGTCAAGATTGCTGGGGCTGATGCTCGTCACGTTGTCGGCTGCGCCGGTCAGCGCCGGCACGCTGTACAA
GTGCGTCGGCGCTGACGGTATCACCAGTTACCTGAGCAAGCGTCAGAGCGGCTCCACCTGCAGCGTCATCAGCAGCTATA
CCCCCGATCGTGCGGCCCGCCGCCTGCCGTCATCTCCCTCGGCCGGGCGTCTGGCCGTCAGCCCCGCGCCCCGCATGGCC
AATGTCGACAGCGGCGCGCCGGCCACCATTATCAGCCAGCCAGTCAGCCAGCCGCCGCGGCACGCTGCCGAGGTCGCTTC
GGCGCCGGCTCCGCGTGCCTTGCCGGAGTCGTCCGTCTCGGTCGTCACACCGCCAGCGGCAGCAGCAACCAATCCGCGCC
GGGTCGTCAGCGGGCAGGTGTACTCTTATATGAAGGACGGCGTGCGCCACTACACCAGCGCCCGGCCGCGCCAGGTCGCC
AGCATCGAAGGCCTGCGCACCATCCATTACAGCTTTATCGAAACCTGCTACGCCTGCGGTGCCAAGCCCGGGGTCAATTT
TGGTGCGATCCGGCTCAACACCAACGCCTATCAGCAGGAAATCGCAGCGGCGGCGCGCGAGTTCGGGGTGGAAGAATCGA
TCGTGCGCGCGATCATCCACGCCGAGTCGGCCTACAACCCGCTCGCGCTCAGCCGTGCCGGCGCGCAAGGCCTGATGCAG
TTGATGCCGGGCACCGCGCGCCGCTTCGGCGTCAGCGATGCCTATGATGCAACGCAAAATATTCGTGGCGGCGTGCAGTA
TCTGTCCTGGCTGCTGAAACGCTTCAATGGCGACCTCACCCTGGCCGCTGCCGGCTACAACGCCGGCGAGGGCGCAGTCG
ACCGTTACGGCGGCGTACCGCCGTATAGCGAAACCCAGCGCTACGTGCAACGCGTGGGCGTATTGGCCGGCCGCTATCGT
GGCCAGGCCACAGCAGCCAACTGA

Upstream 100 bases:

>100_bases
ACCGGTCCCGCACGCCTGGCCGCGGCGCGGGCACCTTGCGGGCTGCTCCAGACGCTGGCCGACACTTGGCAAGCCCTGGG
CAAGCTGGGACACTCCGCAC

Downstream 100 bases:

>100_bases
AGCGGCTGGCAAAACGACCGCGCAGCCGCCCGGCGGGCGCGGCCGGTGCTCCTGGCATGTCATAAGCAGCGCGGCGTCTG
CGCGATCCATTCCCGGCCCC

Product: putative soluble lytic murein transglycosylase precursor

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKGMSRLLGLMLVTLSAAPVSAGTLYKCVGADGITSYLSKRQSGSTCSVISSYTPDRAARRLPSSPSAGRLAVSPAPRMA
NVDSGAPATIISQPVSQPPRHAAEVASAPAPRALPESSVSVVTPPAAAATNPRRVVSGQVYSYMKDGVRHYTSARPRQVA
SIEGLRTIHYSFIETCYACGAKPGVNFGAIRLNTNAYQQEIAAAAREFGVEESIVRAIIHAESAYNPLALSRAGAQGLMQ
LMPGTARRFGVSDAYDATQNIRGGVQYLSWLLKRFNGDLTLAAAGYNAGEGAVDRYGGVPPYSETQRYVQRVGVLAGRYR
GQATAAN

Sequences:

>Translated_327_residues
MKGMSRLLGLMLVTLSAAPVSAGTLYKCVGADGITSYLSKRQSGSTCSVISSYTPDRAARRLPSSPSAGRLAVSPAPRMA
NVDSGAPATIISQPVSQPPRHAAEVASAPAPRALPESSVSVVTPPAAAATNPRRVVSGQVYSYMKDGVRHYTSARPRQVA
SIEGLRTIHYSFIETCYACGAKPGVNFGAIRLNTNAYQQEIAAAAREFGVEESIVRAIIHAESAYNPLALSRAGAQGLMQ
LMPGTARRFGVSDAYDATQNIRGGVQYLSWLLKRFNGDLTLAAAGYNAGEGAVDRYGGVPPYSETQRYVQRVGVLAGRYR
GQATAAN
>Mature_327_residues
MKGMSRLLGLMLVTLSAAPVSAGTLYKCVGADGITSYLSKRQSGSTCSVISSYTPDRAARRLPSSPSAGRLAVSPAPRMA
NVDSGAPATIISQPVSQPPRHAAEVASAPAPRALPESSVSVVTPPAAAATNPRRVVSGQVYSYMKDGVRHYTSARPRQVA
SIEGLRTIHYSFIETCYACGAKPGVNFGAIRLNTNAYQQEIAAAAREFGVEESIVRAIIHAESAYNPLALSRAGAQGLMQ
LMPGTARRFGVSDAYDATQNIRGGVQYLSWLLKRFNGDLTLAAAGYNAGEGAVDRYGGVPPYSETQRYVQRVGVLAGRYR
GQATAAN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=134, Percent_Identity=33.5820895522388, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 34457; Mature: 34457

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00922 TRANSGLYCOSYLASE ; PS00141 ASP_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMSRLLGLMLVTLSAAPVSAGTLYKCVGADGITSYLSKRQSGSTCSVISSYTPDRAAR
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCCHHHHH
RLPSSPSAGRLAVSPAPRMANVDSGAPATIISQPVSQPPRHAAEVASAPAPRALPESSVS
HCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCE
VVTPPAAAATNPRRVVSGQVYSYMKDGVRHYTSARPRQVASIEGLRTIHYSFIETCYACG
EECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AKPGVNFGAIRLNTNAYQQEIAAAAREFGVEESIVRAIIHAESAYNPLALSRAGAQGLMQ
CCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LMPGTARRFGVSDAYDATQNIRGGVQYLSWLLKRFNGDLTLAAAGYNAGEGAVDRYGGVP
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCC
PYSETQRYVQRVGVLAGRYRGQATAAN
CHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKGMSRLLGLMLVTLSAAPVSAGTLYKCVGADGITSYLSKRQSGSTCSVISSYTPDRAAR
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHCCCCHHHHH
RLPSSPSAGRLAVSPAPRMANVDSGAPATIISQPVSQPPRHAAEVASAPAPRALPESSVS
HCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCE
VVTPPAAAATNPRRVVSGQVYSYMKDGVRHYTSARPRQVASIEGLRTIHYSFIETCYACG
EECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
AKPGVNFGAIRLNTNAYQQEIAAAAREFGVEESIVRAIIHAESAYNPLALSRAGAQGLMQ
CCCCCCEEEEEECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LMPGTARRFGVSDAYDATQNIRGGVQYLSWLLKRFNGDLTLAAAGYNAGEGAVDRYGGVP
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHCCCCC
PYSETQRYVQRVGVLAGRYRGQATAAN
CHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]