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The map label for this gene is proB [H]

Identifier: 78048098

GI number: 78048098

Start: 2870040

End: 2871194

Strand: Reverse

Name: proB [H]

Synonym: XCV2542

Alternate gene names: 78048098

Gene position: 2871194-2870040 (Counterclockwise)

Preceding gene: 78048099

Following gene: 78048097

Centisome position: 55.44

GC content: 68.14

Gene sequence:

>1155_bases
ATGAGCACGCCTGCCAGCACGTTGTCGCCGTTTACCGAGCAGGCGCTGCCGCCGTGGCGCCGTGCGGTGCTCAAAGTCGG
CAGCAGCCTGCTCGCCGCCGATGGCGGTGGCCTGTCCCCGCGCTTCGCGCTGGGTCTGGCGCAGTTCGTTTCCGCCAATC
TGGCAGCCGGGCGCGAGCTGGTGATCGTCTCGTCGGGCGCGGTGGCCGCTGGTCGCGCCATCCTGCCCAAAGCGGCGGAT
GTCGGCGCGCCGATCGCGGCGCGGCAGGCACTGGCCGCCTTGGGCCAAGCGCAGCTGATTGCGTTATGGCAGCGCTTTTT
CGAGCGGCCGGTGGCGCAGGTACTGCTCACGCATGACGACTTGCGCAACCGTCGCCGCTATCTCAACGCGCGTGCCACCT
TGGGCGAGTTGCTGCGGCTGGGTGCGCTGCCGGTGATCAACGAAAACGACACCGTCTCGGTGGATGAGTTGAAGCTGGGC
GATAACGACAACCTGGCCGCGATCGTGGCTGCGCTGGTCGATGCCGACGCGTTGTTCATCGCCACCGACATCGATGGGTT
GTATAGCGCCGACCCACGTAGCAATCCGCTGGCGCGCCCGCTCGACGATGTGCCCGAGCTCACCCCTGAAGTGCTGGCGA
TGGCCGGCGGCAGCGGCAGCAACGTGGGGACCGGCGGCATGCGCACCAAACTGGAAGCCGCAGCAAAGGCCGGTGCGGCC
GGCATCGAAACCTATCTATTCAACGGCCGAAGTGGCGAGGTGGTGCGCGCCCTGGCGCAGGACCGCCTGCGTGGCACGCG
CATCCACGCCGCGCGCGCGCGCATCGCTGCGCGAAAATACTGGCTGCGGCACGCACCGGTGGAAGCGGGTGCGATCCTCA
TCGACGACGGCGCCGCAGCGGCCCTGACCGGCAAGGGCGCCTCGCTGTTGCCAGGCGGCGTGGCCGGCGCGCAGGGCGAT
TTCCGTCGCGGCGACATGGTGGAGATTCGTCTGCGCGACACCGCGGGCGACCAGTGTCTGGCACGCGGCGTCAGCCAGTA
TTCGGCCCTGGATATCCGTCGTATCGCAGGCCGCCACTCGCGCGAGATCGAGAACGTGCTCGGCTACAGTTACGGCGAAA
ACGTGGTGCATCGCGATGATCTGGTGGTGTTGTAG

Upstream 100 bases:

>100_bases
CAAGAGGCGCAGCAGATCGTTGCGCGCGACCCGCTGGTGCTGCGCGATGCTTCTGCACTGACCGTGTATGAGTGGAACAC
GCACTGAGGACGCCATTGCC

Downstream 100 bases:

>100_bases
CAGAGATTCGACATTTGGGATTGGGGATTCGCAAAAGCATGTGTGCTGCGCGTTTCACCTTTTGCCGCTTTGTTACAGTG
AATGAGTCCGTTGTGCTAAT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSTPASTLSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGRELVIVSSGAVAAGRAILPKAAD
VGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLG
DNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
GIETYLFNGRSGEVVRALAQDRLRGTRIHAARARIAARKYWLRHAPVEAGAILIDDGAAAALTGKGASLLPGGVAGAQGD
FRRGDMVEIRLRDTAGDQCLARGVSQYSALDIRRIAGRHSREIENVLGYSYGENVVHRDDLVVL

Sequences:

>Translated_384_residues
MSTPASTLSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGRELVIVSSGAVAAGRAILPKAAD
VGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLG
DNDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
GIETYLFNGRSGEVVRALAQDRLRGTRIHAARARIAARKYWLRHAPVEAGAILIDDGAAAALTGKGASLLPGGVAGAQGD
FRRGDMVEIRLRDTAGDQCLARGVSQYSALDIRRIAGRHSREIENVLGYSYGENVVHRDDLVVL
>Mature_383_residues
STPASTLSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGRELVIVSSGAVAAGRAILPKAADV
GAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDDLRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGD
NDNLAAIVAALVDADALFIATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAAG
IETYLFNGRSGEVVRALAQDRLRGTRIHAARARIAARKYWLRHAPVEAGAILIDDGAAAALTGKGASLLPGGVAGAQGDF
RRGDMVEIRLRDTAGDQCLARGVSQYSALDIRRIAGRHSREIENVLGYSYGENVVHRDDLVVL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=283, Percent_Identity=29.3286219081272, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI62912457, Length=281, Percent_Identity=29.1814946619217, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786437, Length=364, Percent_Identity=45.0549450549451, Blast_Score=268, Evalue=6e-73,
Organism=Caenorhabditis elegans, GI17569967, Length=268, Percent_Identity=32.8358208955224, Blast_Score=114, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17569969, Length=268, Percent_Identity=32.8358208955224, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6320506, Length=413, Percent_Identity=36.319612590799, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321822, Length=412, Percent_Identity=35.4368932038835, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21357643, Length=293, Percent_Identity=33.4470989761092, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 40323; Mature: 40192

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPASTLSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREL
CCCCCHHHCHHHHHCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
VIVSSGAVAAGRAILPKAADVGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDD
EEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFI
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEECCCCCCHHHHHHHHHCCCEEEE
ATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
EECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
GIETYLFNGRSGEVVRALAQDRLRGTRIHAARARIAARKYWLRHAPVEAGAILIDDGAAA
CCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
ALTGKGASLLPGGVAGAQGDFRRGDMVEIRLRDTAGDQCLARGVSQYSALDIRRIAGRHS
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHH
REIENVLGYSYGENVVHRDDLVVL
HHHHHHHCCCCCCCCEECCCEEEC
>Mature Secondary Structure 
STPASTLSPFTEQALPPWRRAVLKVGSSLLAADGGGLSPRFALGLAQFVSANLAAGREL
CCCCHHHCHHHHHCCCHHHHHHHHHCCHHEECCCCCCCHHHHHHHHHHHHHHHCCCCEE
VIVSSGAVAAGRAILPKAADVGAPIAARQALAALGQAQLIALWQRFFERPVAQVLLTHDD
EEEECCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNRRRYLNARATLGELLRLGALPVINENDTVSVDELKLGDNDNLAAIVAALVDADALFI
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEHEEEECCCCCCHHHHHHHHHCCCEEEE
ATDIDGLYSADPRSNPLARPLDDVPELTPEVLAMAGGSGSNVGTGGMRTKLEAAAKAGAA
EECCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
GIETYLFNGRSGEVVRALAQDRLRGTRIHAARARIAARKYWLRHAPVEAGAILIDDGAAA
CCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEE
ALTGKGASLLPGGVAGAQGDFRRGDMVEIRLRDTAGDQCLARGVSQYSALDIRRIAGRHS
EEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHH
REIENVLGYSYGENVVHRDDLVVL
HHHHHHHCCCCCCCCEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]