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The map label for this gene is ngoBVM [H]

Identifier: 78047961

GI number: 78047961

Start: 2727933

End: 2729549

Strand: Reverse

Name: ngoBVM [H]

Synonym: XCV2405

Alternate gene names: 78047961

Gene position: 2729549-2727933 (Counterclockwise)

Preceding gene: 78047962

Following gene: 78047960

Centisome position: 52.71

GC content: 68.4

Gene sequence:

>1617_bases
ATGAACCCGCAACCTCGCACCCCACGCGGTGCGCCCCAGGCCGCGCCACTGCTGTACGGCAGCGTGTGCAGCGGCATCGA
GGCCGTGAGCCTCGCCTGGCAGCCCATCGGCCTCGAAGCCGCGTGGTTCGCCGAGATCGAGCCGTTCCCGAGCGCCGTGC
TCGCCCACCGTTACCCCCGCGTGCCCAACCTGGGCGACATGACCGCGATCGCCCGCCAGGTGCGCGCCGGCACCGTGCCG
GCGCCCGACATCCTGGTCGGCGGCACGCCGTGCCAGTCGTTCAGCGTTGCAGGTAGCCGTCGCGGGCTGGATGACCCGCG
CGGAGCCCTTTCCCTTGCCTATGTGGAGCTTGCCAATGCCATCGACCAAATCCGCCACCAAGACCGCCGCCCCGCGGCCA
CACTCGTCTGGGAAAACGTCCCCGGCGTGCTCAATGACCGCTGCAATGCCTTCGGTCAATTCCTCGCTGCACTGGCCGGA
GAAAGCCGTGCGCTCCAGCCGCCAGGGGGTCGGTGGGCGCACGCTGGTTGTGTGTCTGGACCCCGCCGCCGCATCGCCTG
GCGCGTGCTCGACGCTCAATATTTCGGTGTCGCCCAACGCCGCAAGCGCGTGTTTCTTGTGGCAAGTGGTGGAAATGACC
TCGATCCCGCAGAAGTACTTTTTGAGCGCGCAGGCCTGCTCGGGGATTCTTGTGCGGGCCGCGCGCCGTGGCAAGAAGCT
GCCCACGATGCTGGACCGGGTGCTGAAGCAGCAGGCGCATGCGCAGGACTTGCAGGATTCGCAGGACTGAAGCAGCCCTA
CGGCAAGATCACGACGACGTTCGGATTCAGCGGCGGCATTGGCCCTGTCGATGTGGCGGCATGCCTGATGGCCGCTGGCC
CCAAGCACGACATCCGCACCGAGACGTTCATGGTGCAGTCGGTCGCCGGCAGCATCGCCCACACCCTCGACACCGCCAAC
AACGGCAAGGGCAGCAGCGAGGACGGCACCGGCAAGGGCGTTCCGATCATCGCCTTCACCGCCCAGGGCAGCGGCGCGGA
TGCCACGCTCGACCTGACGCCGACGCTGCGCGCTGGCGGCCATCGCAACAGCCATGCGAACGCCGGGGTCGTGCCCGCCA
TCGCGTTCGCGCAGAACAACCGCGGTGAAGTGCGCTTCGAGTCGGGCCATGGCCAGGTCGCTTGCACCGTGCTGTCCAAC
GGCAAGCCGGGCTACGGCGTGCCTATGGTGGCCTGCGTGTCTCTGCGCGGCCGCGAGCAGGGTCTTGCCGCCGAGCTGGG
CGGCACCGTAGCGGGCGCACTGCGCACCAGCGGCGGCGGTGCGGACAAGCCCTATGTCCTGGCTCCCGACTTCGAGGCGC
ATTTCCGCTACGACTGGAACGACCCTGGCCCCGGCGACTGGTCGCACTGGCGTGTGCGGCGGTTGATGCCCGTGGAGTGC
GAGCGGCTGCAAGGTATGCCCGACGACTACACGCTGATCCCGTACCGCGGCAAGCCCGCCGCCGACGCTCCACGCTACAA
GGCGATCGGCAACTCCATGGCTGTCCCGTGCATGGCATGGCTGGGCCAGCGCCTGGTGCAGTACCTGCACAAGACGGGCT
CAACCGCTTCGGATTGA

Upstream 100 bases:

>100_bases
CTCCCGAGCCGAAGGCCGCAAGGCCTTCGGCGCCTTTCTCCTGCCAATCAACGTCCGGCCAGGCCACACGGTCCAGGGCC
ACACGAACAGGAGACCCGCC

Downstream 100 bases:

>100_bases
TCGGCGACGCAGCCTGCGGGCCGCGCCATTCTCCCTCCTGCATTGCCGATGGTTCGGCAGCAGCCCGCAGCCAGGGTGTC
AAGGCTGCCGCGGGTTCCAC

Product: DNA methyltransferase

Products: NA

Alternate protein names: M.NgoBV; Cytosine-specific methyltransferase NgoBV; M.NgoV [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MNPQPRTPRGAPQAAPLLYGSVCSGIEAVSLAWQPIGLEAAWFAEIEPFPSAVLAHRYPRVPNLGDMTAIARQVRAGTVP
APDILVGGTPCQSFSVAGSRRGLDDPRGALSLAYVELANAIDQIRHQDRRPAATLVWENVPGVLNDRCNAFGQFLAALAG
ESRALQPPGGRWAHAGCVSGPRRRIAWRVLDAQYFGVAQRRKRVFLVASGGNDLDPAEVLFERAGLLGDSCAGRAPWQEA
AHDAGPGAEAAGACAGLAGFAGLKQPYGKITTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSIAHTLDTAN
NGKGSSEDGTGKGVPIIAFTAQGSGADATLDLTPTLRAGGHRNSHANAGVVPAIAFAQNNRGEVRFESGHGQVACTVLSN
GKPGYGVPMVACVSLRGREQGLAAELGGTVAGALRTSGGGADKPYVLAPDFEAHFRYDWNDPGPGDWSHWRVRRLMPVEC
ERLQGMPDDYTLIPYRGKPAADAPRYKAIGNSMAVPCMAWLGQRLVQYLHKTGSTASD

Sequences:

>Translated_538_residues
MNPQPRTPRGAPQAAPLLYGSVCSGIEAVSLAWQPIGLEAAWFAEIEPFPSAVLAHRYPRVPNLGDMTAIARQVRAGTVP
APDILVGGTPCQSFSVAGSRRGLDDPRGALSLAYVELANAIDQIRHQDRRPAATLVWENVPGVLNDRCNAFGQFLAALAG
ESRALQPPGGRWAHAGCVSGPRRRIAWRVLDAQYFGVAQRRKRVFLVASGGNDLDPAEVLFERAGLLGDSCAGRAPWQEA
AHDAGPGAEAAGACAGLAGFAGLKQPYGKITTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSIAHTLDTAN
NGKGSSEDGTGKGVPIIAFTAQGSGADATLDLTPTLRAGGHRNSHANAGVVPAIAFAQNNRGEVRFESGHGQVACTVLSN
GKPGYGVPMVACVSLRGREQGLAAELGGTVAGALRTSGGGADKPYVLAPDFEAHFRYDWNDPGPGDWSHWRVRRLMPVEC
ERLQGMPDDYTLIPYRGKPAADAPRYKAIGNSMAVPCMAWLGQRLVQYLHKTGSTASD
>Mature_538_residues
MNPQPRTPRGAPQAAPLLYGSVCSGIEAVSLAWQPIGLEAAWFAEIEPFPSAVLAHRYPRVPNLGDMTAIARQVRAGTVP
APDILVGGTPCQSFSVAGSRRGLDDPRGALSLAYVELANAIDQIRHQDRRPAATLVWENVPGVLNDRCNAFGQFLAALAG
ESRALQPPGGRWAHAGCVSGPRRRIAWRVLDAQYFGVAQRRKRVFLVASGGNDLDPAEVLFERAGLLGDSCAGRAPWQEA
AHDAGPGAEAAGACAGLAGFAGLKQPYGKITTTFGFSGGIGPVDVAACLMAAGPKHDIRTETFMVQSVAGSIAHTLDTAN
NGKGSSEDGTGKGVPIIAFTAQGSGADATLDLTPTLRAGGHRNSHANAGVVPAIAFAQNNRGEVRFESGHGQVACTVLSN
GKPGYGVPMVACVSLRGREQGLAAELGGTVAGALRTSGGGADKPYVLAPDFEAHFRYDWNDPGPGDWSHWRVRRLMPVEC
ERLQGMPDDYTLIPYRGKPAADAPRYKAIGNSMAVPCMAWLGQRLVQYLHKTGSTASD

Specific function: This methylase recognizes the double-stranded sequence GGNNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoBV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 56452; Mature: 56452

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPQPRTPRGAPQAAPLLYGSVCSGIEAVSLAWQPIGLEAAWFAEIEPFPSAVLAHRYPR
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHEEECCCCCCEEEEECCCCCHHHHHHHCCCC
VPNLGDMTAIARQVRAGTVPAPDILVGGTPCQSFSVAGSRRGLDDPRGALSLAYVELANA
CCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IDQIRHQDRRPAATLVWENVPGVLNDRCNAFGQFLAALAGESRALQPPGGRWAHAGCVSG
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCC
PRRRIAWRVLDAQYFGVAQRRKRVFLVASGGNDLDPAEVLFERAGLLGDSCAGRAPWQEA
CHHEEEEEEECHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHH
AHDAGPGAEAAGACAGLAGFAGLKQPYGKITTTFGFSGGIGPVDVAACLMAAGPKHDIRT
HCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCH
ETFMVQSVAGSIAHTLDTANNGKGSSEDGTGKGVPIIAFTAQGSGADATLDLTPTLRAGG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCHHCCC
HRNSHANAGVVPAIAFAQNNRGEVRFESGHGQVACTVLSNGKPGYGVPMVACVSLRGREQ
CCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCC
GLAAELGGTVAGALRTSGGGADKPYVLAPDFEAHFRYDWNDPGPGDWSHWRVRRLMPVEC
CCHHHHCCHHHHHHCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCH
ERLQGMPDDYTLIPYRGKPAADAPRYKAIGNSMAVPCMAWLGQRLVQYLHKTGSTASD
HHHCCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPQPRTPRGAPQAAPLLYGSVCSGIEAVSLAWQPIGLEAAWFAEIEPFPSAVLAHRYPR
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHEEECCCCCCEEEEECCCCCHHHHHHHCCCC
VPNLGDMTAIARQVRAGTVPAPDILVGGTPCQSFSVAGSRRGLDDPRGALSLAYVELANA
CCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IDQIRHQDRRPAATLVWENVPGVLNDRCNAFGQFLAALAGESRALQPPGGRWAHAGCVSG
HHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCC
PRRRIAWRVLDAQYFGVAQRRKRVFLVASGGNDLDPAEVLFERAGLLGDSCAGRAPWQEA
CHHEEEEEEECHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHH
AHDAGPGAEAAGACAGLAGFAGLKQPYGKITTTFGFSGGIGPVDVAACLMAAGPKHDIRT
HCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCH
ETFMVQSVAGSIAHTLDTANNGKGSSEDGTGKGVPIIAFTAQGSGADATLDLTPTLRAGG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCHHCCC
HRNSHANAGVVPAIAFAQNNRGEVRFESGHGQVACTVLSNGKPGYGVPMVACVSLRGREQ
CCCCCCCCCCCEEEEEECCCCCCEEEECCCCEEEEEEEECCCCCCCCHHHHHHHHCCCCC
GLAAELGGTVAGALRTSGGGADKPYVLAPDFEAHFRYDWNDPGPGDWSHWRVRRLMPVEC
CCHHHHCCHHHHHHCCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCH
ERLQGMPDDYTLIPYRGKPAADAPRYKAIGNSMAVPCMAWLGQRLVQYLHKTGSTASD
HHHCCCCCCEEEEEECCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]