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The map label for this gene is yogA [H]

Identifier: 78047726

GI number: 78047726

Start: 2496105

End: 2497076

Strand: Reverse

Name: yogA [H]

Synonym: XCV2170

Alternate gene names: 78047726

Gene position: 2497076-2496105 (Counterclockwise)

Preceding gene: 78047727

Following gene: 78047719

Centisome position: 48.22

GC content: 62.96

Gene sequence:

>972_bases
ATGTTTGCCGTCTACGCCAAAGAAGCCAACGTCGACGACCCGCTTGCCTCCGTCGTCGTCGGAGAGCGCCCCGAGCCCAT
CGTGCAAGAGGGCTGGGTACGCGTGAAGGTGACCCATGCCAGCCTTAACCGGCACGACATTTTCACCCTGCGCGGCATGA
CCTCCCAGGAGCAGCCGATCCCCTTCCCGATGATCCTGGGCAACGATGCCGCAGGCGTGCTCGAAGACGGCACGCCCGTC
GTGCTCTATCCGGTACTGGGCAGCGACGACTGGAAAGGCGACGAGACCCTCGACCCGCACTGGCACGTTATCAGCGAACT
CGTGCCCGGGACGATGGCCGATTATGTCGCCGTCCCGAAACGCAACGCGGTCCCTATTCCTGACGGTTTGTCACCGCTTC
ACGCCTCGCTGCTCGGCACCGCGTGGCTCACGGCCTATCGCTCGCTCTTTACCAAATCACGGTTGATTCCGGGGCAGACG
CTTCTCGTACAGGGCGCTTCGGGCGGTATGTCGACGGCGCTCATACAGATGGGCCGTGCTGCCGGCTTCGAAGTGTGGGT
CACCACCCGCAATGATGAAGGGGCCGCCATTGCCGAACGCCTTGGCGCCAACCGGGTATTGCGACATGGCGAGGCCTTGC
CACGCCGCGTTGACGCAGTCGTCGACAACATCGGCGCGGCGACATGGGCAGATAGCCTGAAGGCGGTCAAGCGCGGTGGC
GTCCTGGTGATCAACGGCATCACCACCGGGAACATCGCCGAAACCGACGTGCTGCGCATCTTCGTCGAACAGATCGACAT
CCGCGGCACCATCATGGGCACGCTTGAGGAAATGAAGGCCATGATGCAATTCGTCATCCGCAACAACATCGCCCCCGAAG
TCGGAGCGGTGGTGCCGATGACCGAAGCCCGCGACGCGATCCGCAACATGATCGACGGTCGCACCCAGGGCAAGACCGTG
TTCACGAGATAA

Upstream 100 bases:

>100_bases
ATTTCAACTGCGGTACCGCAGGGCAAACCTTGCGCCAGCGCTCACTTCGACTTCACGTGCGCGCCGCACAAAACGTCCAA
CCGACTGAAGGAGAACGACC

Downstream 100 bases:

>100_bases
TTGGTAGAGCAGCGTAAAGCACAGAAGCAAAAGCCCGCCCAACGGAGCTTTCGCTGGCTAAAACACTACCCGAACTTGCC
AGACCCAAAAACCCAAAGCC

Product: putative oxidoreductase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFAVYAKEANVDDPLASVVVGERPEPIVQEGWVRVKVTHASLNRHDIFTLRGMTSQEQPIPFPMILGNDAAGVLEDGTPV
VLYPVLGSDDWKGDETLDPHWHVISELVPGTMADYVAVPKRNAVPIPDGLSPLHASLLGTAWLTAYRSLFTKSRLIPGQT
LLVQGASGGMSTALIQMGRAAGFEVWVTTRNDEGAAIAERLGANRVLRHGEALPRRVDAVVDNIGAATWADSLKAVKRGG
VLVINGITTGNIAETDVLRIFVEQIDIRGTIMGTLEEMKAMMQFVIRNNIAPEVGAVVPMTEARDAIRNMIDGRTQGKTV
FTR

Sequences:

>Translated_323_residues
MFAVYAKEANVDDPLASVVVGERPEPIVQEGWVRVKVTHASLNRHDIFTLRGMTSQEQPIPFPMILGNDAAGVLEDGTPV
VLYPVLGSDDWKGDETLDPHWHVISELVPGTMADYVAVPKRNAVPIPDGLSPLHASLLGTAWLTAYRSLFTKSRLIPGQT
LLVQGASGGMSTALIQMGRAAGFEVWVTTRNDEGAAIAERLGANRVLRHGEALPRRVDAVVDNIGAATWADSLKAVKRGG
VLVINGITTGNIAETDVLRIFVEQIDIRGTIMGTLEEMKAMMQFVIRNNIAPEVGAVVPMTEARDAIRNMIDGRTQGKTV
FTR
>Mature_323_residues
MFAVYAKEANVDDPLASVVVGERPEPIVQEGWVRVKVTHASLNRHDIFTLRGMTSQEQPIPFPMILGNDAAGVLEDGTPV
VLYPVLGSDDWKGDETLDPHWHVISELVPGTMADYVAVPKRNAVPIPDGLSPLHASLLGTAWLTAYRSLFTKSRLIPGQT
LLVQGASGGMSTALIQMGRAAGFEVWVTTRNDEGAAIAERLGANRVLRHGEALPRRVDAVVDNIGAATWADSLKAVKRGG
VLVINGITTGNIAETDVLRIFVEQIDIRGTIMGTLEEMKAMMQFVIRNNIAPEVGAVVPMTEARDAIRNMIDGRTQGKTV
FTR

Specific function: Preferred Specificity Is Towards 1-Propanol. [C]

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=333, Percent_Identity=26.4264264264264, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI22538444, Length=333, Percent_Identity=26.4264264264264, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI194239674, Length=307, Percent_Identity=25.0814332247557, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI13236495, Length=307, Percent_Identity=25.0814332247557, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI194239676, Length=292, Percent_Identity=24.6575342465753, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI28557745, Length=158, Percent_Identity=28.4810126582279, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=190, Percent_Identity=28.9473684210526, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71988145, Length=321, Percent_Identity=24.6105919003115, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.1.1.1

Molecular weight: Translated: 34950; Mature: 34950

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAVYAKEANVDDPLASVVVGERPEPIVQEGWVRVKVTHASLNRHDIFTLRGMTSQEQPI
CEEEEECCCCCCCHHHHHHCCCCCCCHHHCCEEEEEEEECCCCCCEEEEEECCCCCCCCC
PFPMILGNDAAGVLEDGTPVVLYPVLGSDDWKGDETLDPHWHVISELVPGTMADYVAVPK
CCEEEECCCCCCEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCC
RNAVPIPDGLSPLHASLLGTAWLTAYRSLFTKSRLIPGQTLLVQGASGGMSTALIQMGRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCC
AGFEVWVTTRNDEGAAIAERLGANRVLRHGEALPRRVDAVVDNIGAATWADSLKAVKRGG
CCEEEEEEECCCCCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
VLVINGITTGNIAETDVLRIFVEQIDIRGTIMGTLEEMKAMMQFVIRNNIAPEVGAVVPM
EEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TEARDAIRNMIDGRTQGKTVFTR
HHHHHHHHHHHCCCCCCCEECCC
>Mature Secondary Structure
MFAVYAKEANVDDPLASVVVGERPEPIVQEGWVRVKVTHASLNRHDIFTLRGMTSQEQPI
CEEEEECCCCCCCHHHHHHCCCCCCCHHHCCEEEEEEEECCCCCCEEEEEECCCCCCCCC
PFPMILGNDAAGVLEDGTPVVLYPVLGSDDWKGDETLDPHWHVISELVPGTMADYVAVPK
CCEEEECCCCCCEECCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCC
RNAVPIPDGLSPLHASLLGTAWLTAYRSLFTKSRLIPGQTLLVQGASGGMSTALIQMGRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCC
AGFEVWVTTRNDEGAAIAERLGANRVLRHGEALPRRVDAVVDNIGAATWADSLKAVKRGG
CCEEEEEEECCCCCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
VLVINGITTGNIAETDVLRIFVEQIDIRGTIMGTLEEMKAMMQFVIRNNIAPEVGAVVPM
EEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TEARDAIRNMIDGRTQGKTVFTR
HHHHHHHHHHHCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]