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The map label for this gene is flgJ [H]

Identifier: 78047581

GI number: 78047581

Start: 2307318

End: 2308496

Strand: Reverse

Name: flgJ [H]

Synonym: XCV2025

Alternate gene names: 78047581

Gene position: 2308496-2307318 (Counterclockwise)

Preceding gene: 78047582

Following gene: 78047580

Centisome position: 44.58

GC content: 66.58

Gene sequence:

>1179_bases
ATGCGTATCGCAGCCTCGCCCATTGATCTCAACCCGAGCACCAAGGCCGATCCGGCAAAGATCGACAAGGTCTCCCGTCA
GCTCGAAGGTCAGTTCGCGCAGATGCTGGTCAAGAGCATGCGCGATGCGAGCTCCGGCGACCCGATGTTTCCGGGCCAAA
ACCAGATGTTCCGCGAAATGTACGACCAGCAGATGGCCAAGGCGTTGACCGACGGCAAGGGGCTGGGACTGTCGGCGATG
ATCTCCAAGCAGCTCAGTGGCGACACGGGCGGCCCGGCCTTGAACACCTCGTTGAACACTGCCGAAGCGGCCAAGGCGTA
CGCCCTGGTTGCCGGCAAGCGCGACGCCTCGTTGCCGCTGCCCGCCCGCGACGGCGCGGCCACCGGCATCACGACCAGCA
GCGTGGCGAAGGCGGCCCTGGGCGCAGGCAACTTGAGCGGCATCGGCATGAGCCAGGTGCTGGATCTGATTGCCGGGCGT
ACCGGCGCTGGCGAAGCCGGCAGCGACGATGCTGCAGCACTGAGCTGGCCGTCGGCCAACGATCGCTGGGCCGATGTGGC
CGCCAGCGATGCAGCCGATGCCAATGCCGCGGTCAATGCGAGCGCCGCCAGCAGCGCCGCTGCCAGCCTGGGCGAACGCA
CCCCGGAAGGCTTTGTCGCCAAGATCTGGACGCATGCGCAAAAGGCTGCGCGCGAACTGGGTGTGGACCCGCGCGCGCTG
GTGGCCCAGGCCGCATTGGAAACCGGTTGGGGCCGTCGCGGCATCGGCAACGGTGGCGACTCCAACAACCTGTTCGGCAT
CAAGGCCACCGGCTGGAGCGGCGACAAGGTCACCACCGGCACCCACGAATACGTCAACGGCGTCAAAACCACCGAAACCG
CCGATTTCCGCGCCTATGGCTCGGCCGAGGAGAGTTTTGCCGACTACGTGCGGCTGCTGAAGAACAACAGCCGTTACCAG
CCCGCGCTGCAGGCCGGCACCGATATCAAGGGTTTTGCACGCGGCTTGCAACAAGCCGGATACGCCACCGATCCGGGCTA
TGCGGCCAAGATCGCGGCGATCGCCAACGGCCCGACCATCGACCGTGCGGTGGCCGCCATCGGCAACGCGGCGGCCGATC
TGTCCAACCGCTATGCCAGTACCGTCGAGCCGGTCGGGCTCGGCACCATCCGTCGCTGA

Upstream 100 bases:

>100_bases
GCAGTCAACGAAGTGGGCGCCGCGCCCGGCGACCTGGTGGCCATCCTGGAAGCGCTCAAGCAAGCCGGTGCGCTGACGGC
GGAGCTAGAGGTGATCTGAC

Downstream 100 bases:

>100_bases
GGTAAACGCTCATGTCCATCATGTCCACCGGGACCAGCGCGCTCATCGCCTTCCAACGGGCGTTGTCGACCGTTAGCCAT
AACGTCGCCAACATCAATAC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREMYDQQMAKALTDGKGLGLSAM
ISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPLPARDGAATGITTSSVAKAALGAGNLSGIGMSQVLDLIAGR
TGAGEAGSDDAAALSWPSANDRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYGSAEESFADYVRLLKNNSRYQ
PALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTIDRAVAAIGNAAADLSNRYASTVEPVGLGTIRR

Sequences:

>Translated_392_residues
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREMYDQQMAKALTDGKGLGLSAM
ISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPLPARDGAATGITTSSVAKAALGAGNLSGIGMSQVLDLIAGR
TGAGEAGSDDAAALSWPSANDRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYGSAEESFADYVRLLKNNSRYQ
PALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTIDRAVAAIGNAAADLSNRYASTVEPVGLGTIRR
>Mature_392_residues
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREMYDQQMAKALTDGKGLGLSAM
ISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPLPARDGAATGITTSSVAKAALGAGNLSGIGMSQVLDLIAGR
TGAGEAGSDDAAALSWPSANDRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYGSAEESFADYVRLLKNNSRYQ
PALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTIDRAVAAIGNAAADLSNRYASTVEPVGLGTIRR

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=139, Percent_Identity=46.0431654676259, Blast_Score=108, Evalue=4e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 40171; Mature: 40171

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YDQQMAKALTDGKGLGLSAMISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCC
PARDGAATGITTSSVAKAALGAGNLSGIGMSQVLDLIAGRTGAGEAGSDDAAALSWPSAN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
DRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYG
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHCC
SAEESFADYVRLLKNNSRYQPALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTI
CHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEEEECCCCCHH
DRAVAAIGNAAADLSNRYASTVEPVGLGTIRR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YDQQMAKALTDGKGLGLSAMISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCC
PARDGAATGITTSSVAKAALGAGNLSGIGMSQVLDLIAGRTGAGEAGSDDAAALSWPSAN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
DRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYG
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHCC
SAEESFADYVRLLKNNSRYQPALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTI
CHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEEEECCCCCHH
DRAVAAIGNAAADLSNRYASTVEPVGLGTIRR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]